Trapping effect of eugenol on hydroxyl radicals induced by L-DOPA in Vitro

Masahiro Ogata, Daisuke Kaneya, Kazuo Shin-Ya, Linxang Li, Yoshihiro Abe, Hironobu Katoh, Sachiko Seki, Yasuhide Seki, Ryoko Gonda, Shiro Urano, Toyoshige Endo

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12 Citations (Scopus)

Abstract

Many researchers have stated that eugenol might inhibit lipid peroxidation at the stage of initiation, propagation, or both, and many attempts have been made to elucidate the mechanism of its antioxidant activity. Nevertheless, details of its mechanism are still obscure. This study was carried out to investigate the trapping effect of eugenol on hydroxyl radical generated from L-3,4-dihydroxyphenylalanine (DOPA) in MiliQ water and the generation mechanism of the hydroxyl radical by this system which uses no metallic factor. This was studied by adding L-DOPA and 5,5-dimethyl-1-pyrroline N-oxide (DMPO) to phosphate buffered saline (PBS) or MiliQ water, and the generation of hydroxyl radical was detected on an ESR spectrum. By this method, the effect of antioxidants was detected as a modification of ESR spectra. We found that the eugenol trapped hydroxyl radicals directly, because it had no iron chelating action, did not trap L-DOPA semiquinone radical and inhibited hydroxyl radicals with or without iron ion.

Original languageEnglish
Pages (from-to)1167-1170
Number of pages4
JournalChemical and Pharmaceutical Bulletin
Volume53
Issue number9
DOIs
Publication statusPublished - 2005 Sep 1

Keywords

  • ESR
  • Eugenol
  • Hydroxyl radical
  • L-3,4-dihydroxyphenylalanine (dopa)

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

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    Ogata, M., Kaneya, D., Shin-Ya, K., Li, L., Abe, Y., Katoh, H., Seki, S., Seki, Y., Gonda, R., Urano, S., & Endo, T. (2005). Trapping effect of eugenol on hydroxyl radicals induced by L-DOPA in Vitro. Chemical and Pharmaceutical Bulletin, 53(9), 1167-1170. https://doi.org/10.1248/cpb.53.1167