Trapping effect of eugenol on hydroxyl radicals induced by L-DOPA in Vitro

Masahiro Ogata, Daisuke Kaneya, Kazuo Shin-Ya, Linxang Li, Yoshihiro Abe, Hironobu Katoh, Sachiko Seki, Yasuhide Seki, Ryoko Gonda, Shiro Urano, Toyoshige Endo

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Many researchers have stated that eugenol might inhibit lipid peroxidation at the stage of initiation, propagation, or both, and many attempts have been made to elucidate the mechanism of its antioxidant activity. Nevertheless, details of its mechanism are still obscure. This study was carried out to investigate the trapping effect of eugenol on hydroxyl radical generated from L-3,4-dihydroxyphenylalanine (DOPA) in MiliQ water and the generation mechanism of the hydroxyl radical by this system which uses no metallic factor. This was studied by adding L-DOPA and 5,5-dimethyl-1-pyrroline N-oxide (DMPO) to phosphate buffered saline (PBS) or MiliQ water, and the generation of hydroxyl radical was detected on an ESR spectrum. By this method, the effect of antioxidants was detected as a modification of ESR spectra. We found that the eugenol trapped hydroxyl radicals directly, because it had no iron chelating action, did not trap L-DOPA semiquinone radical and inhibited hydroxyl radicals with or without iron ion.

Original languageEnglish
Pages (from-to)1167-1170
Number of pages4
JournalChemical and Pharmaceutical Bulletin
Volume53
Issue number9
DOIs
Publication statusPublished - 2005 Sep

Fingerprint

Eugenol
Dihydroxyphenylalanine
Hydroxyl Radical
Paramagnetic resonance
Iron
Antioxidants
Water
Levodopa
Chelation
Lipid Peroxidation
Phosphates
Research Personnel
In Vitro Techniques
Ions
Lipids

Keywords

  • ESR
  • Eugenol
  • Hydroxyl radical
  • L-3,4-dihydroxyphenylalanine (dopa)

ASJC Scopus subject areas

  • Drug Discovery
  • Organic Chemistry
  • Chemistry(all)
  • Pharmacology

Cite this

Ogata, M., Kaneya, D., Shin-Ya, K., Li, L., Abe, Y., Katoh, H., ... Endo, T. (2005). Trapping effect of eugenol on hydroxyl radicals induced by L-DOPA in Vitro. Chemical and Pharmaceutical Bulletin, 53(9), 1167-1170. https://doi.org/10.1248/cpb.53.1167

Trapping effect of eugenol on hydroxyl radicals induced by L-DOPA in Vitro. / Ogata, Masahiro; Kaneya, Daisuke; Shin-Ya, Kazuo; Li, Linxang; Abe, Yoshihiro; Katoh, Hironobu; Seki, Sachiko; Seki, Yasuhide; Gonda, Ryoko; Urano, Shiro; Endo, Toyoshige.

In: Chemical and Pharmaceutical Bulletin, Vol. 53, No. 9, 09.2005, p. 1167-1170.

Research output: Contribution to journalArticle

Ogata, M, Kaneya, D, Shin-Ya, K, Li, L, Abe, Y, Katoh, H, Seki, S, Seki, Y, Gonda, R, Urano, S & Endo, T 2005, 'Trapping effect of eugenol on hydroxyl radicals induced by L-DOPA in Vitro', Chemical and Pharmaceutical Bulletin, vol. 53, no. 9, pp. 1167-1170. https://doi.org/10.1248/cpb.53.1167
Ogata, Masahiro ; Kaneya, Daisuke ; Shin-Ya, Kazuo ; Li, Linxang ; Abe, Yoshihiro ; Katoh, Hironobu ; Seki, Sachiko ; Seki, Yasuhide ; Gonda, Ryoko ; Urano, Shiro ; Endo, Toyoshige. / Trapping effect of eugenol on hydroxyl radicals induced by L-DOPA in Vitro. In: Chemical and Pharmaceutical Bulletin. 2005 ; Vol. 53, No. 9. pp. 1167-1170.
@article{7b7ff79b253d4b23b9285e54332cd54b,
title = "Trapping effect of eugenol on hydroxyl radicals induced by L-DOPA in Vitro",
abstract = "Many researchers have stated that eugenol might inhibit lipid peroxidation at the stage of initiation, propagation, or both, and many attempts have been made to elucidate the mechanism of its antioxidant activity. Nevertheless, details of its mechanism are still obscure. This study was carried out to investigate the trapping effect of eugenol on hydroxyl radical generated from L-3,4-dihydroxyphenylalanine (DOPA) in MiliQ water and the generation mechanism of the hydroxyl radical by this system which uses no metallic factor. This was studied by adding L-DOPA and 5,5-dimethyl-1-pyrroline N-oxide (DMPO) to phosphate buffered saline (PBS) or MiliQ water, and the generation of hydroxyl radical was detected on an ESR spectrum. By this method, the effect of antioxidants was detected as a modification of ESR spectra. We found that the eugenol trapped hydroxyl radicals directly, because it had no iron chelating action, did not trap L-DOPA semiquinone radical and inhibited hydroxyl radicals with or without iron ion.",
keywords = "ESR, Eugenol, Hydroxyl radical, L-3,4-dihydroxyphenylalanine (dopa)",
author = "Masahiro Ogata and Daisuke Kaneya and Kazuo Shin-Ya and Linxang Li and Yoshihiro Abe and Hironobu Katoh and Sachiko Seki and Yasuhide Seki and Ryoko Gonda and Shiro Urano and Toyoshige Endo",
year = "2005",
month = "9",
doi = "10.1248/cpb.53.1167",
language = "English",
volume = "53",
pages = "1167--1170",
journal = "Chemical and Pharmaceutical Bulletin",
issn = "0009-2363",
publisher = "Pharmaceutical Society of Japan",
number = "9",

}

TY - JOUR

T1 - Trapping effect of eugenol on hydroxyl radicals induced by L-DOPA in Vitro

AU - Ogata, Masahiro

AU - Kaneya, Daisuke

AU - Shin-Ya, Kazuo

AU - Li, Linxang

AU - Abe, Yoshihiro

AU - Katoh, Hironobu

AU - Seki, Sachiko

AU - Seki, Yasuhide

AU - Gonda, Ryoko

AU - Urano, Shiro

AU - Endo, Toyoshige

PY - 2005/9

Y1 - 2005/9

N2 - Many researchers have stated that eugenol might inhibit lipid peroxidation at the stage of initiation, propagation, or both, and many attempts have been made to elucidate the mechanism of its antioxidant activity. Nevertheless, details of its mechanism are still obscure. This study was carried out to investigate the trapping effect of eugenol on hydroxyl radical generated from L-3,4-dihydroxyphenylalanine (DOPA) in MiliQ water and the generation mechanism of the hydroxyl radical by this system which uses no metallic factor. This was studied by adding L-DOPA and 5,5-dimethyl-1-pyrroline N-oxide (DMPO) to phosphate buffered saline (PBS) or MiliQ water, and the generation of hydroxyl radical was detected on an ESR spectrum. By this method, the effect of antioxidants was detected as a modification of ESR spectra. We found that the eugenol trapped hydroxyl radicals directly, because it had no iron chelating action, did not trap L-DOPA semiquinone radical and inhibited hydroxyl radicals with or without iron ion.

AB - Many researchers have stated that eugenol might inhibit lipid peroxidation at the stage of initiation, propagation, or both, and many attempts have been made to elucidate the mechanism of its antioxidant activity. Nevertheless, details of its mechanism are still obscure. This study was carried out to investigate the trapping effect of eugenol on hydroxyl radical generated from L-3,4-dihydroxyphenylalanine (DOPA) in MiliQ water and the generation mechanism of the hydroxyl radical by this system which uses no metallic factor. This was studied by adding L-DOPA and 5,5-dimethyl-1-pyrroline N-oxide (DMPO) to phosphate buffered saline (PBS) or MiliQ water, and the generation of hydroxyl radical was detected on an ESR spectrum. By this method, the effect of antioxidants was detected as a modification of ESR spectra. We found that the eugenol trapped hydroxyl radicals directly, because it had no iron chelating action, did not trap L-DOPA semiquinone radical and inhibited hydroxyl radicals with or without iron ion.

KW - ESR

KW - Eugenol

KW - Hydroxyl radical

KW - L-3,4-dihydroxyphenylalanine (dopa)

UR - http://www.scopus.com/inward/record.url?scp=24944562247&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=24944562247&partnerID=8YFLogxK

U2 - 10.1248/cpb.53.1167

DO - 10.1248/cpb.53.1167

M3 - Article

C2 - 16141589

AN - SCOPUS:24944562247

VL - 53

SP - 1167

EP - 1170

JO - Chemical and Pharmaceutical Bulletin

JF - Chemical and Pharmaceutical Bulletin

SN - 0009-2363

IS - 9

ER -