Treatment of dry eye by autologous serum application in Sjogren's syndrome

Kazuo Tsubota, Eiki Goto, Hiromi Fujita, Masafumi Ono, Hiroko Inoue, Ichiro Saito, Shigeto Shimmura

Research output: Contribution to journalArticle

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Abstract

Aim - To evaluate the efficacy of autologous serum application for the treatment of dry eye in Sjogren's syndrome. Methods - The stability of essential components (EGF, vitamin A, and TGF-β) in preserved serum were examined following preservation at 4°C and -20°C. In a primary clinical trial, 12 patients with Sjogren's syndrome were treated with autologous serum (diluted to 20% with sterile saline) for 4 weeks, and vital staining of the ocular surface was compared before and after treatment. The effects of serum on mucin (MUC-1) expression were observed in cultured conjunctival epithelial cells in vitro. Results - EGF, vitamin A, and TGF-β were well preserved for up to 1 month in the refrigerator at 4°C and up to 3 months in the freezer at -20°C. Rose bengal and fluorescein scores improved significantly from the initial scores of 5.3 and 5.6 to 1.7 and 2.5 after 4 weeks, respectively. The additive effect of human serum for cultured conjunctival epithelial cells showed significant MUC-1 upregulation on the cell surface. Conclusion - Autologous serum application is a safe and efficient way to provide essential components to the ocular surface in the treatment of dry eye associated with Sjogren's syndrome.

Original languageEnglish
Pages (from-to)390-395
Number of pages6
JournalBritish Journal of Ophthalmology
Volume83
Issue number4
Publication statusPublished - 1999

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Sjogren's Syndrome
Serum
Vitamin A
Epidermal Growth Factor
Therapeutics
Epithelial Cells
Rose Bengal
Mucin-1
Fluorescein
Up-Regulation
Clinical Trials
Staining and Labeling

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Treatment of dry eye by autologous serum application in Sjogren's syndrome. / Tsubota, Kazuo; Goto, Eiki; Fujita, Hiromi; Ono, Masafumi; Inoue, Hiroko; Saito, Ichiro; Shimmura, Shigeto.

In: British Journal of Ophthalmology, Vol. 83, No. 4, 1999, p. 390-395.

Research output: Contribution to journalArticle

Tsubota, K, Goto, E, Fujita, H, Ono, M, Inoue, H, Saito, I & Shimmura, S 1999, 'Treatment of dry eye by autologous serum application in Sjogren's syndrome', British Journal of Ophthalmology, vol. 83, no. 4, pp. 390-395.
Tsubota, Kazuo ; Goto, Eiki ; Fujita, Hiromi ; Ono, Masafumi ; Inoue, Hiroko ; Saito, Ichiro ; Shimmura, Shigeto. / Treatment of dry eye by autologous serum application in Sjogren's syndrome. In: British Journal of Ophthalmology. 1999 ; Vol. 83, No. 4. pp. 390-395.
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AU - Inoue, Hiroko

AU - Saito, Ichiro

AU - Shimmura, Shigeto

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