TY - JOUR
T1 - Trim43a, Trim43b, and Trim43c
T2 - Novel mouse genes expressed specifically in mouse preimplantation embryos
AU - Stanghellini, Ilaria
AU - Falco, Geppino
AU - Lee, Sung Lim
AU - Monti, Manuela
AU - Ko, Minoru S.H.
N1 - Funding Information:
We thank Dr. Toshio Hamatani for the initial contribution to the work, Dr. Yuhki Nakatake for the discussion, Ms. Donna Tignor and Dr. Suresh Poosala for help in mouse husbandry, and Mr. Dawood Dudekula for help in DNA sequence analysis. We also thank Dr. Gary Felsenfeld for a generous gift of the insulator DNA, Dr. Roger Tsien for a generous gift of mStrawberry, and Ms. Lois Maltais for help in assigning gene symbols. This work was supported entirely by the Intramural Research Program of the National Institute on Aging, NIH.
PY - 2009/12
Y1 - 2009/12
N2 - We describe the identification and characterization of Trim43a, Trim43b, and Trim43c genes, whose expression are restricted to preimplantation stages and peak at the 8-cell to morula stage. We identified a 5 kb DNA fragment that covers upstream region of Trim43a as a putative promoter, which can drive the expression of mStrawberry fluorescent protein in a manner similar to endogenous Trim43 genes. Trim43 genes will be useful stage-specific markers for the study of preimplantation embryos.
AB - We describe the identification and characterization of Trim43a, Trim43b, and Trim43c genes, whose expression are restricted to preimplantation stages and peak at the 8-cell to morula stage. We identified a 5 kb DNA fragment that covers upstream region of Trim43a as a putative promoter, which can drive the expression of mStrawberry fluorescent protein in a manner similar to endogenous Trim43 genes. Trim43 genes will be useful stage-specific markers for the study of preimplantation embryos.
KW - 8-Cell
KW - Asymmetric PCR
KW - Fluorescent proteins
KW - Gene expression
KW - Morula
KW - Mouse preimplantation
KW - Promoter analysis
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U2 - 10.1016/j.gep.2009.08.002
DO - 10.1016/j.gep.2009.08.002
M3 - Article
C2 - 19703589
AN - SCOPUS:70350743016
SN - 1567-133X
VL - 9
SP - 595
EP - 602
JO - Gene Expression Patterns
JF - Gene Expression Patterns
IS - 8
ER -