Trophinin and tastin, a novel cell adhesion molecule complex with potential involvement in embryo implantation

M. N. Fukuda, T. Sato, J. Nakayama, G. Klier, M. Mikami, Daisuke Aoki, S. Nozawa

Research output: Contribution to journalArticle

158 Citations (Scopus)

Abstract

Two human epithelial cell lines, trophoblastic teratocarcinoma HT-H and endometrial adenocarcinoma SNG-M cells, adhere to each other at their respective apical cell surfaces in a divalent cation-independent manner. Two novel molecules responsible for the adhesion between these two cell types were identified by expression cDNA cloning. One, named trophinin, is an intrinsic membrane protein and mediates homophilic serf-binding. Another, named tastin, is a cytoplasmic protein and is necessary for trophinin to function as a cell adhesion molecule. Trophinin and tastin appear to be associated with the cytoskeleton in HT-H and SNG-M cells. These molecules are normally not expressed in various types of human cells in tissues, with the exception of macrophages. Strong expression of these molecules was detected in the trophectoderm surface of monkey blastocyst. These molecules are also expressed in human endometrial surface epithelium on day 16/17 at the early secretory phase of human endometrium, the time consistent with that expected for the 'implantation window.'

Original languageEnglish
Pages (from-to)1199-1210
Number of pages12
JournalGenes and Development
Volume9
Issue number10
Publication statusPublished - 1995

Fingerprint

Cell Adhesion Molecules
Teratocarcinoma
Divalent Cations
Blastocyst
Endometrium
Cytoskeleton
Haplorhini
Organism Cloning
Membrane Proteins
Adenocarcinoma
Epithelium
Complementary DNA
Epithelial Cells
Macrophages
Cell Line
Proteins

Keywords

  • cytoskeleton
  • embryo
  • molecular cloning
  • polarized epithelium
  • Trophoblast

ASJC Scopus subject areas

  • Developmental Biology
  • Genetics

Cite this

Fukuda, M. N., Sato, T., Nakayama, J., Klier, G., Mikami, M., Aoki, D., & Nozawa, S. (1995). Trophinin and tastin, a novel cell adhesion molecule complex with potential involvement in embryo implantation. Genes and Development, 9(10), 1199-1210.

Trophinin and tastin, a novel cell adhesion molecule complex with potential involvement in embryo implantation. / Fukuda, M. N.; Sato, T.; Nakayama, J.; Klier, G.; Mikami, M.; Aoki, Daisuke; Nozawa, S.

In: Genes and Development, Vol. 9, No. 10, 1995, p. 1199-1210.

Research output: Contribution to journalArticle

Fukuda, MN, Sato, T, Nakayama, J, Klier, G, Mikami, M, Aoki, D & Nozawa, S 1995, 'Trophinin and tastin, a novel cell adhesion molecule complex with potential involvement in embryo implantation', Genes and Development, vol. 9, no. 10, pp. 1199-1210.
Fukuda, M. N. ; Sato, T. ; Nakayama, J. ; Klier, G. ; Mikami, M. ; Aoki, Daisuke ; Nozawa, S. / Trophinin and tastin, a novel cell adhesion molecule complex with potential involvement in embryo implantation. In: Genes and Development. 1995 ; Vol. 9, No. 10. pp. 1199-1210.
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