Trophoblast cell activation by trophinin ligation is implicated in human embryo implantation

Kazuhiro Sugihara, Daijiro Sugiyama, James Byrne, Don P. Wolf, Kevin P. Lowitz, Yoichi Kobayashi, Maryam Kabir-Salmani, Daita Nadano, Daisuke Aoki, Shiro Nozawa, Jun Nakayama, Tomas Mustelin, Erkki Ruoslahti, Naoto Yamaguchi, Michiko N. Fukuda

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

During human embryo implantation, trophectoderm mediates adhesion of the blastocyst to the uterine epithelium. The rapid growth of the embryo and invasion of the maternal tissue suggest adhesion-induced activation of the embryonal cells. We show here that ligation of trophinin, a homophilic cell adhesion molecule expressed on trophoblastic cells, induces tyrosine phosphorylation in trophinin-expressing trophoblastic HT-H cells. The phosphorylation could be induced in HT-H cells with the binding of trophinin-expressing cells or anti trophinin antibodies. Trophinin-dependent tyrosine phosphorylation was associated with actin reorganization. We also isolated trophinin-binding peptides from phage libraries. These peptides exhibited the consensus sequence GWRQ and seemed to reproduce the effects of trophinin-mediated cell adhesion. Upon binding of a GWRQ peptide, HT-H cells became highly proliferative and motile. HT-H cells expressed ErbB family receptors and bound EGF and heparin-binding EGF-like growth factor (HB-EGF), but ErbB family receptor phosphorylation in these cells required GWRQ. In the absence of GWRQ, trophinin interacted with the cytoplasmic protein bystin, which binds to ErbB4 and blocks its autophosphorylation. In HT-H cells, GWRQ peptide dissociated trophinin from bystin, and ErbB4 was activated. Culturing monkey blastocysts in the presence of the peptide increased total number and motility of the trophectoderm cells. These results suggest that trophinin-mediated cell adhesion functions as a molecular switch for trophectoderm activation in human embryo implantation.

Original languageEnglish
Pages (from-to)3799-3804
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number10
DOIs
Publication statusPublished - 2007 Mar 6

Fingerprint

Trophoblasts
Ligation
Phosphorylation
Peptides
Blastocyst
Cell Adhesion
Tyrosine
Tissue Adhesions
Peptide Library
Consensus Sequence
Cell Adhesion Molecules
Epidermal Growth Factor
Bacteriophages
Cell Movement
Haplorhini
Actins
Anti-Idiotypic Antibodies
Embryonic Structures
Epithelium
Mothers

Keywords

  • BYSL
  • ErbB4
  • Pregnancy
  • Receptor tyrosine kinase
  • Stem cells

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Trophoblast cell activation by trophinin ligation is implicated in human embryo implantation. / Sugihara, Kazuhiro; Sugiyama, Daijiro; Byrne, James; Wolf, Don P.; Lowitz, Kevin P.; Kobayashi, Yoichi; Kabir-Salmani, Maryam; Nadano, Daita; Aoki, Daisuke; Nozawa, Shiro; Nakayama, Jun; Mustelin, Tomas; Ruoslahti, Erkki; Yamaguchi, Naoto; Fukuda, Michiko N.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, No. 10, 06.03.2007, p. 3799-3804.

Research output: Contribution to journalArticle

Sugihara, K, Sugiyama, D, Byrne, J, Wolf, DP, Lowitz, KP, Kobayashi, Y, Kabir-Salmani, M, Nadano, D, Aoki, D, Nozawa, S, Nakayama, J, Mustelin, T, Ruoslahti, E, Yamaguchi, N & Fukuda, MN 2007, 'Trophoblast cell activation by trophinin ligation is implicated in human embryo implantation', Proceedings of the National Academy of Sciences of the United States of America, vol. 104, no. 10, pp. 3799-3804. https://doi.org/10.1073/pnas.0611516104
Sugihara, Kazuhiro ; Sugiyama, Daijiro ; Byrne, James ; Wolf, Don P. ; Lowitz, Kevin P. ; Kobayashi, Yoichi ; Kabir-Salmani, Maryam ; Nadano, Daita ; Aoki, Daisuke ; Nozawa, Shiro ; Nakayama, Jun ; Mustelin, Tomas ; Ruoslahti, Erkki ; Yamaguchi, Naoto ; Fukuda, Michiko N. / Trophoblast cell activation by trophinin ligation is implicated in human embryo implantation. In: Proceedings of the National Academy of Sciences of the United States of America. 2007 ; Vol. 104, No. 10. pp. 3799-3804.
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AU - Kobayashi, Yoichi

AU - Kabir-Salmani, Maryam

AU - Nadano, Daita

AU - Aoki, Daisuke

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AU - Nakayama, Jun

AU - Mustelin, Tomas

AU - Ruoslahti, Erkki

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N2 - During human embryo implantation, trophectoderm mediates adhesion of the blastocyst to the uterine epithelium. The rapid growth of the embryo and invasion of the maternal tissue suggest adhesion-induced activation of the embryonal cells. We show here that ligation of trophinin, a homophilic cell adhesion molecule expressed on trophoblastic cells, induces tyrosine phosphorylation in trophinin-expressing trophoblastic HT-H cells. The phosphorylation could be induced in HT-H cells with the binding of trophinin-expressing cells or anti trophinin antibodies. Trophinin-dependent tyrosine phosphorylation was associated with actin reorganization. We also isolated trophinin-binding peptides from phage libraries. These peptides exhibited the consensus sequence GWRQ and seemed to reproduce the effects of trophinin-mediated cell adhesion. Upon binding of a GWRQ peptide, HT-H cells became highly proliferative and motile. HT-H cells expressed ErbB family receptors and bound EGF and heparin-binding EGF-like growth factor (HB-EGF), but ErbB family receptor phosphorylation in these cells required GWRQ. In the absence of GWRQ, trophinin interacted with the cytoplasmic protein bystin, which binds to ErbB4 and blocks its autophosphorylation. In HT-H cells, GWRQ peptide dissociated trophinin from bystin, and ErbB4 was activated. Culturing monkey blastocysts in the presence of the peptide increased total number and motility of the trophectoderm cells. These results suggest that trophinin-mediated cell adhesion functions as a molecular switch for trophectoderm activation in human embryo implantation.

AB - During human embryo implantation, trophectoderm mediates adhesion of the blastocyst to the uterine epithelium. The rapid growth of the embryo and invasion of the maternal tissue suggest adhesion-induced activation of the embryonal cells. We show here that ligation of trophinin, a homophilic cell adhesion molecule expressed on trophoblastic cells, induces tyrosine phosphorylation in trophinin-expressing trophoblastic HT-H cells. The phosphorylation could be induced in HT-H cells with the binding of trophinin-expressing cells or anti trophinin antibodies. Trophinin-dependent tyrosine phosphorylation was associated with actin reorganization. We also isolated trophinin-binding peptides from phage libraries. These peptides exhibited the consensus sequence GWRQ and seemed to reproduce the effects of trophinin-mediated cell adhesion. Upon binding of a GWRQ peptide, HT-H cells became highly proliferative and motile. HT-H cells expressed ErbB family receptors and bound EGF and heparin-binding EGF-like growth factor (HB-EGF), but ErbB family receptor phosphorylation in these cells required GWRQ. In the absence of GWRQ, trophinin interacted with the cytoplasmic protein bystin, which binds to ErbB4 and blocks its autophosphorylation. In HT-H cells, GWRQ peptide dissociated trophinin from bystin, and ErbB4 was activated. Culturing monkey blastocysts in the presence of the peptide increased total number and motility of the trophectoderm cells. These results suggest that trophinin-mediated cell adhesion functions as a molecular switch for trophectoderm activation in human embryo implantation.

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