@article{447ef3396f184b7693f482e2992adc55,
title = "Tryptophan Metabolism Regulates Proliferative Capacity of Human Pluripotent Stem Cells",
abstract = "Cell Biology; Metabolomics; Stem Cell Research",
keywords = "Cell Biology, Metabolomics, Stem Cell Research",
author = "Shota Someya and Shugo Tohyama and Kotaro Kameda and Sho Tanosaki and Yuika Morita and Kazunori Sasaki and Kang, {Moon Il} and Yoshikazu Kishino and Marina Okada and Hidenori Tani and Yusuke Soma and Kazuaki Nakajima and Tomohiko Umei and Otoya Sekine and Taijun Moriwaki and Hideaki Kanazawa and Eiji Kobayashi and Jun Fujita and Keiichi Fukuda",
note = "Funding Information: The authors thank Drs. Satoru Okamoto and Kotoe Koseki for analysis of amino acids consumption profiles in culture media (Ajinomoto); Tomoko Haruna, Rei Ohno, Sayaka Kanaami, Chihana Fujita, Miho Yamaguchi, Yui Narita, and Naoko Matsumoto for technical assistance with cell preparation and culture (Department of Cardiology, Keio University); Dr. Erina Sugita for technical assistance with Extracellular Flux Analyzer (Department of Endocrinology, Metabolism and Nephrology, Keio University); and Dr. Kenichiro Kinouchi for fruitful discussions (Department of Endocrinology, Metabolism and Nephrology, Keio University). The authors also thank the Center for iPSC Research and Application, and Kyoto University, for the 253G4 and 201B7 cell lines, and WiCell research institute for H9 ES cells. The present work was mainly supported by Projects for Technological Development, Research Center Network for Realization of Regenerative Medicine by Japan, the Japan Agency for Medical Research and Development (AMED) grant 20bm0404023h0003 to S. Tohyama, and partly supported by the Highway Program for Realization of Regenerative Medicine from AMED grant 19bk0104062h0003 to K.F. HMT Research Grant for Young Leaders in Metabolomics to S. Tohyama, and the Japan Society for the Promotion of Science (JSPS) KAKENHI 19K22626 to S. Tohyama. S. Tohyama and J.F. conceptualized the study; S. Tohyama designed the study; S.S. performed and analyzed most experiments; K.K. S.Tanosaki, Y.M. K.S. M.-I.K. Y.K. M.O. H.T. Y.S. K.N. T.U. O.S. T.M. and H.K. contributed to specific experiments; S. Tohyama and S.S. wrote the original draft; S. Tohyama, J.F. and K.F. wrote, reviewed, and edited the manuscript; S. Tohyama and K.F acquired funding; S. Tohyama, J.F. and K.F. supervised the study. S.S. S. Tohyama, J.F. and K.F. have a patent pending related to this work. K.F. is a co-founder and CEO of Heartseed, Inc. S. Tohyama is an advisor of Heartseed, Inc. S. Tohyama, J.F. H.K. and K.F. own equity in Heartseed, Inc. The remaining authors have no conflicts of interest to disclose. Funding Information: The authors thank Drs. Satoru Okamoto and Kotoe Koseki for analysis of amino acids consumption profiles in culture media (Ajinomoto); Tomoko Haruna, Rei Ohno, Sayaka Kanaami, Chihana Fujita, Miho Yamaguchi, Yui Narita, and Naoko Matsumoto for technical assistance with cell preparation and culture (Department of Cardiology, Keio University); Dr. Erina Sugita for technical assistance with Extracellular Flux Analyzer (Department of Endocrinology, Metabolism and Nephrology, Keio University); and Dr. Kenichiro Kinouchi for fruitful discussions (Department of Endocrinology, Metabolism and Nephrology, Keio University). The authors also thank the Center for iPSC Research and Application, and Kyoto University, for the 253G4 and 201B7 cell lines, and WiCell research institute for H9 ES cells. The present work was mainly supported by Projects for Technological Development, Research Center Network for Realization of Regenerative Medicine by Japan, the Japan Agency for Medical Research and Development (AMED) grant 20bm0404023h0003 to S. Tohyama, and partly supported by the Highway Program for Realization of Regenerative Medicine from AMED grant 19bk0104062h0003 to K.F., HMT Research Grant for Young Leaders in Metabolomics to S. Tohyama, and the Japan Society for the Promotion of Science ( JSPS) KAKENHI 19K22626 to S. Tohyama. Publisher Copyright: {\textcopyright} 2021 The Author(s)",
year = "2021",
month = feb,
day = "19",
doi = "10.1016/j.isci.2021.102090",
language = "English",
volume = "24",
journal = "iScience",
issn = "2589-0042",
publisher = "Elsevier Inc.",
number = "2",
}