Tryptophan Metabolism Regulates Proliferative Capacity of Human Pluripotent Stem Cells

Shota Someya; Shugo Tohyama; Kotaro Kameda; Sho Tanosaki; Yuika Morita; Kazunori Sasaki; Moon Il Kang; Yoshikazu Kishino; Marina Okada; Hidenori Tani; Yusuke Soma; Kazuaki Nakajima; Tomohiko Umei; Otoya Sekine; Taijun Moriwaki; Hideaki Kanazawa; Eiji Kobayashi; Jun Fujita; Keiichi Fukuda

doi:10.1016/j.isci.2021.102090

Tryptophan Metabolism Regulates Proliferative Capacity of Human Pluripotent Stem Cells

Shota Someya, Shugo Tohyama, Kotaro Kameda, Sho Tanosaki, Yuika Morita, Kazunori Sasaki, Moon Il Kang, Yoshikazu Kishino, Marina Okada, Hidenori Tani, Yusuke Soma, Kazuaki Nakajima, Tomohiko Umei, Otoya Sekine, Taijun Moriwaki, Hideaki Kanazawa, Eiji Kobayashi, Jun Fujita, Keiichi Fukuda

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Cell

Original language	English
Article number	102090
Journal	iScience
Volume	24
Issue number	2
DOIs	https://doi.org/10.1016/j.isci.2021.102090
Publication status	Published - 2021 Feb 19

Keywords

Cell Biology
Metabolomics
Stem Cell Research

ASJC Scopus subject areas

General

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10.1016/j.isci.2021.102090

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Someya, S., Tohyama, S., Kameda, K., Tanosaki, S., Morita, Y., Sasaki, K., Kang, M. I., Kishino, Y., Okada, M., Tani, H., Soma, Y., Nakajima, K., Umei, T., Sekine, O., Moriwaki, T., Kanazawa, H., Kobayashi, E., Fujita, J., & Fukuda, K. (2021). Tryptophan Metabolism Regulates Proliferative Capacity of Human Pluripotent Stem Cells. iScience, 24(2), [102090]. https://doi.org/10.1016/j.isci.2021.102090

Someya, S, Tohyama, S, Kameda, K, Tanosaki, S, Morita, Y, Sasaki, K, Kang, MI, Kishino, Y, Okada, M, Tani, H, Soma, Y, Nakajima, K, Umei, T, Sekine, O, Moriwaki, T, Kanazawa, H, Kobayashi, E, Fujita, J & Fukuda, K 2021, 'Tryptophan Metabolism Regulates Proliferative Capacity of Human Pluripotent Stem Cells', iScience, vol. 24, no. 2, 102090. https://doi.org/10.1016/j.isci.2021.102090

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title = "Tryptophan Metabolism Regulates Proliferative Capacity of Human Pluripotent Stem Cells",

abstract = "Cell",

keywords = "Cell Biology, Metabolomics, Stem Cell Research",

author = "Shota Someya and Shugo Tohyama and Kotaro Kameda and Sho Tanosaki and Yuika Morita and Kazunori Sasaki and Kang, {Moon Il} and Yoshikazu Kishino and Marina Okada and Hidenori Tani and Yusuke Soma and Kazuaki Nakajima and Tomohiko Umei and Otoya Sekine and Taijun Moriwaki and Hideaki Kanazawa and Eiji Kobayashi and Jun Fujita and Keiichi Fukuda",

note = "Funding Information: The authors thank Drs. Satoru Okamoto and Kotoe Koseki for analysis of amino acids consumption profiles in culture media (Ajinomoto); Tomoko Haruna, Rei Ohno, Sayaka Kanaami, Chihana Fujita, Miho Yamaguchi, Yui Narita, and Naoko Matsumoto for technical assistance with cell preparation and culture (Department of Cardiology, Keio University); Dr. Erina Sugita for technical assistance with Extracellular Flux Analyzer (Department of Endocrinology, Metabolism and Nephrology, Keio University); and Dr. Kenichiro Kinouchi for fruitful discussions (Department of Endocrinology, Metabolism and Nephrology, Keio University). The authors also thank the Center for iPSC Research and Application, and Kyoto University, for the 253G4 and 201B7 cell lines, and WiCell research institute for H9 ES cells. The present work was mainly supported by Projects for Technological Development, Research Center Network for Realization of Regenerative Medicine by Japan, the Japan Agency for Medical Research and Development (AMED) grant 20bm0404023h0003 to S. Tohyama, and partly supported by the Highway Program for Realization of Regenerative Medicine from AMED grant 19bk0104062h0003 to K.F. HMT Research Grant for Young Leaders in Metabolomics to S. Tohyama, and the Japan Society for the Promotion of Science (JSPS) KAKENHI 19K22626 to S. Tohyama. S. Tohyama and J.F. conceptualized the study; S. Tohyama designed the study; S.S. performed and analyzed most experiments; K.K. S.Tanosaki, Y.M. K.S. M.-I.K. Y.K. M.O. H.T. Y.S. K.N. T.U. O.S. T.M. and H.K. contributed to specific experiments; S. Tohyama and S.S. wrote the original draft; S. Tohyama, J.F. and K.F. wrote, reviewed, and edited the manuscript; S. Tohyama and K.F acquired funding; S. Tohyama, J.F. and K.F. supervised the study. S.S. S. Tohyama, J.F. and K.F. have a patent pending related to this work. K.F. is a co-founder and CEO of Heartseed, Inc. S. Tohyama is an advisor of Heartseed, Inc. S. Tohyama, J.F. H.K. and K.F. own equity in Heartseed, Inc. The remaining authors have no conflicts of interest to disclose. Funding Information: The authors thank Drs. Satoru Okamoto and Kotoe Koseki for analysis of amino acids consumption profiles in culture media (Ajinomoto); Tomoko Haruna, Rei Ohno, Sayaka Kanaami, Chihana Fujita, Miho Yamaguchi, Yui Narita, and Naoko Matsumoto for technical assistance with cell preparation and culture (Department of Cardiology, Keio University); Dr. Erina Sugita for technical assistance with Extracellular Flux Analyzer (Department of Endocrinology, Metabolism and Nephrology, Keio University); and Dr. Kenichiro Kinouchi for fruitful discussions (Department of Endocrinology, Metabolism and Nephrology, Keio University). The authors also thank the Center for iPSC Research and Application, and Kyoto University, for the 253G4 and 201B7 cell lines, and WiCell research institute for H9 ES cells. The present work was mainly supported by Projects for Technological Development, Research Center Network for Realization of Regenerative Medicine by Japan, the Japan Agency for Medical Research and Development (AMED) grant 20bm0404023h0003 to S. Tohyama, and partly supported by the Highway Program for Realization of Regenerative Medicine from AMED grant 19bk0104062h0003 to K.F., HMT Research Grant for Young Leaders in Metabolomics to S. Tohyama, and the Japan Society for the Promotion of Science ( JSPS) KAKENHI 19K22626 to S. Tohyama. Publisher Copyright: {\textcopyright} 2021 The Author(s)",

year = "2021",

month = feb,

day = "19",

doi = "10.1016/j.isci.2021.102090",

language = "English",

volume = "24",

journal = "iScience",

issn = "2589-0042",

publisher = "Elsevier Inc.",

number = "2",

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TY - JOUR

T1 - Tryptophan Metabolism Regulates Proliferative Capacity of Human Pluripotent Stem Cells

AU - Someya, Shota

AU - Tohyama, Shugo

AU - Kameda, Kotaro

AU - Tanosaki, Sho

AU - Morita, Yuika

AU - Sasaki, Kazunori

AU - Kang, Moon Il

AU - Kishino, Yoshikazu

AU - Okada, Marina

AU - Tani, Hidenori

AU - Soma, Yusuke

AU - Nakajima, Kazuaki

AU - Umei, Tomohiko

AU - Sekine, Otoya

AU - Moriwaki, Taijun

AU - Kanazawa, Hideaki

AU - Kobayashi, Eiji

AU - Fujita, Jun

AU - Fukuda, Keiichi

N1 - Funding Information: The authors thank Drs. Satoru Okamoto and Kotoe Koseki for analysis of amino acids consumption profiles in culture media (Ajinomoto); Tomoko Haruna, Rei Ohno, Sayaka Kanaami, Chihana Fujita, Miho Yamaguchi, Yui Narita, and Naoko Matsumoto for technical assistance with cell preparation and culture (Department of Cardiology, Keio University); Dr. Erina Sugita for technical assistance with Extracellular Flux Analyzer (Department of Endocrinology, Metabolism and Nephrology, Keio University); and Dr. Kenichiro Kinouchi for fruitful discussions (Department of Endocrinology, Metabolism and Nephrology, Keio University). The authors also thank the Center for iPSC Research and Application, and Kyoto University, for the 253G4 and 201B7 cell lines, and WiCell research institute for H9 ES cells. The present work was mainly supported by Projects for Technological Development, Research Center Network for Realization of Regenerative Medicine by Japan, the Japan Agency for Medical Research and Development (AMED) grant 20bm0404023h0003 to S. Tohyama, and partly supported by the Highway Program for Realization of Regenerative Medicine from AMED grant 19bk0104062h0003 to K.F. HMT Research Grant for Young Leaders in Metabolomics to S. Tohyama, and the Japan Society for the Promotion of Science (JSPS) KAKENHI 19K22626 to S. Tohyama. S. Tohyama and J.F. conceptualized the study; S. Tohyama designed the study; S.S. performed and analyzed most experiments; K.K. S.Tanosaki, Y.M. K.S. M.-I.K. Y.K. M.O. H.T. Y.S. K.N. T.U. O.S. T.M. and H.K. contributed to specific experiments; S. Tohyama and S.S. wrote the original draft; S. Tohyama, J.F. and K.F. wrote, reviewed, and edited the manuscript; S. Tohyama and K.F acquired funding; S. Tohyama, J.F. and K.F. supervised the study. S.S. S. Tohyama, J.F. and K.F. have a patent pending related to this work. K.F. is a co-founder and CEO of Heartseed, Inc. S. Tohyama is an advisor of Heartseed, Inc. S. Tohyama, J.F. H.K. and K.F. own equity in Heartseed, Inc. The remaining authors have no conflicts of interest to disclose. Funding Information: The authors thank Drs. Satoru Okamoto and Kotoe Koseki for analysis of amino acids consumption profiles in culture media (Ajinomoto); Tomoko Haruna, Rei Ohno, Sayaka Kanaami, Chihana Fujita, Miho Yamaguchi, Yui Narita, and Naoko Matsumoto for technical assistance with cell preparation and culture (Department of Cardiology, Keio University); Dr. Erina Sugita for technical assistance with Extracellular Flux Analyzer (Department of Endocrinology, Metabolism and Nephrology, Keio University); and Dr. Kenichiro Kinouchi for fruitful discussions (Department of Endocrinology, Metabolism and Nephrology, Keio University). The authors also thank the Center for iPSC Research and Application, and Kyoto University, for the 253G4 and 201B7 cell lines, and WiCell research institute for H9 ES cells. The present work was mainly supported by Projects for Technological Development, Research Center Network for Realization of Regenerative Medicine by Japan, the Japan Agency for Medical Research and Development (AMED) grant 20bm0404023h0003 to S. Tohyama, and partly supported by the Highway Program for Realization of Regenerative Medicine from AMED grant 19bk0104062h0003 to K.F., HMT Research Grant for Young Leaders in Metabolomics to S. Tohyama, and the Japan Society for the Promotion of Science ( JSPS) KAKENHI 19K22626 to S. Tohyama. Publisher Copyright: © 2021 The Author(s)

PY - 2021/2/19

Y1 - 2021/2/19

N2 - Cell

AB - Cell

KW - Cell Biology

KW - Metabolomics

KW - Stem Cell Research

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DO - 10.1016/j.isci.2021.102090

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VL - 24

JO - iScience

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Tryptophan Metabolism Regulates Proliferative Capacity of Human Pluripotent Stem Cells

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