Tube-Shaped In-Vitro Intestinal Gut Model with 3D Isotropic Medium Supply for Bacterial Symbiosis

Mitsuki Kawahara, Shun Itai, Hiroaki Onoe

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper describes a tube-shaped in vitro intestinal gut model achieving both isotropic medium supply and stable co-culture with bacteria. Our tube-shaped device consists of a collagen/Matrigel tube as a scaffold for intestinal cells and glass/silicone tubes at both ends for perfusion. We confirmed intestinal cells (Caco-2) can be maintained in the luminal part with nutrient-free buffer flow mimicking bacterial suspensions and demonstrated the co-culture of Escherichia-coli (E. coli) in the Caco-2 cultured tube-shaped device. We believe this device provides ple but stable co-culture model with intestinal cells and bacteria and contributes to medical field research relating intestinal bacteria.

Original languageEnglish
Title of host publication35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022
PublisherIEEE Computer Society
Pages321-324
Number of pages4
ISBN (Electronic)9781665409117
DOIs
Publication statusPublished - 2022
Event35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022 - Tokyo, Japan
Duration: 2022 Jan 92022 Jan 13

Publication series

NameIEEE Symposium on Mass Storage Systems and Technologies
Volume2022-January
ISSN (Print)2160-1968

Conference

Conference35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022
Country/TerritoryJapan
CityTokyo
Period22/1/922/1/13

Keywords

  • Cell culturing
  • In vitro 3D tissue
  • Intestinal bacteria
  • Micro tube
  • Tissue engineering

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

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