Tublogenesis of endothelial cells in core-shell hydrogel microfibers

Hiroaki Onoe, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This paper describes tube formation of endothelial cells in collagen/alginate core-shell hydrogel microfiber. We found that encapsulated MS1 (mouse pancreatic endothelial cell line) and HUVEC (human umbilical vein endothelial cell) spontaneously create tube-like monolayer cell structures along the axial direction of the fiber at meter-length scale. Because the produced endothelial tubes are free-standing and handleable in a culture medium with the support of the alginate shell, our endothelial cell fibers would be a fundamental tissue unit for constructing three-dimensional (3D) macroscopic tissues with vascular network and for analyzing cellular behaviors (ex. vasculogenesis or angiogenesis) under intercellular/chemical/mechanical/fluidic interactions.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1069-1071
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012
Externally publishedYes
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 2012 Oct 282012 Nov 1

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period12/10/2812/11/1

Fingerprint

Hydrogel
Endothelial cells
Hydrogels
Alginate
Tissue
Fibers
Fluidics
Collagen
Culture Media
Monolayers
alginic acid

Keywords

  • Cell encapsulation
  • Endothelial cell
  • Hydrogel
  • Microfiber
  • Vascular

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Onoe, H., & Takeuchi, S. (2012). Tublogenesis of endothelial cells in core-shell hydrogel microfibers. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 1069-1071). Chemical and Biological Microsystems Society.

Tublogenesis of endothelial cells in core-shell hydrogel microfibers. / Onoe, Hiroaki; Takeuchi, Shoji.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 1069-1071.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Onoe, H & Takeuchi, S 2012, Tublogenesis of endothelial cells in core-shell hydrogel microfibers. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, pp. 1069-1071, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, Japan, 12/10/28.
Onoe H, Takeuchi S. Tublogenesis of endothelial cells in core-shell hydrogel microfibers. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 1069-1071
Onoe, Hiroaki ; Takeuchi, Shoji. / Tublogenesis of endothelial cells in core-shell hydrogel microfibers. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 1069-1071
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