Tublogenesis of endothelial cells in core-shell hydrogel microfibers

Hiroaki Onoe, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This paper describes tube formation of endothelial cells in collagen/alginate core-shell hydrogel microfiber. We found that encapsulated MS1 (mouse pancreatic endothelial cell line) and HUVEC (human umbilical vein endothelial cell) spontaneously create tube-like monolayer cell structures along the axial direction of the fiber at meter-length scale. Because the produced endothelial tubes are free-standing and handleable in a culture medium with the support of the alginate shell, our endothelial cell fibers would be a fundamental tissue unit for constructing three-dimensional (3D) macroscopic tissues with vascular network and for analyzing cellular behaviors (ex. vasculogenesis or angiogenesis) under intercellular/chemical/mechanical/fluidic interactions.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1069-1071
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012 Jan 1
Externally publishedYes
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 2012 Oct 282012 Nov 1

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period12/10/2812/11/1

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Keywords

  • Cell encapsulation
  • Endothelial cell
  • Hydrogel
  • Microfiber
  • Vascular

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Onoe, H., & Takeuchi, S. (2012). Tublogenesis of endothelial cells in core-shell hydrogel microfibers. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 1069-1071). (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012). Chemical and Biological Microsystems Society.