Tumor antigens isolated from a patient with vitiligo and T-cell-infiltrated melanoma

Y. Kiniwa, T. Fujita, M. Akada, K. Ito, T. Shofuda, Yuriko Matsuzaki, A. Yamamoto, T. Saida, Yutaka Kawakami

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Serological identification of tumor antigens by cDNA expression cloning is a technique used to isolate cDNAs encoding tumor antigens that are recognized by IgG antibodies in sera from cancer patients. It is also useful for the isolation of tumor antigens recognized by T cells. We applied this method to identify melanoma antigens recognized by the serum from a patient with a good prognosis who had T-cell-infiltrated melanoma and vitiligo. By screening a λ phage cDNA library constructed from a highly pigmented melanoma cell line, SKme123, with the patient's serum, 50 positive cDNA clones consisting of 26 distinct antigens were isolated. Of these, 20 encoded known proteins, and 6 encoded previously uncharacterized ones. The most frequently isolated clone, which we named KU-MEL-1, was unknown previously but was homologous to partial cDNA sequences registered in the expressed sequence tag database. Reverse transcription-PCR and Northern blot analysis demonstrated that KU-MEL-1 was strongly expressed in most melanoma cell lines, melanoma tissue samples, and cultured melanocytes and weakly expressed in cell lines derived from other types of tumors, as well as in some normal tissues, including testis. Western blot analysis with polyclonal murine antibody generated by immunization with the recombinant KU-MEL-1 protein demonstrated that the KU-MEL-1 protein was preferentially expressed in melanoma cells and melanocytes IgG antibodies against KU-MEL-1 were detected in the sera from 9 of 26 melanoma patients and from some patients with other cancers including brain tumor, esophageal cancer, colon cancer, and chronic myelogenous leukemia, but were not detected in sera from 30 healthy individuals. Although the IgG specific for KU-MEL-1 was not detected in sera from 12 vitiligo patients, it was detected in sera from 7 of 11 patients with Vogt-Koyanagi-Harada disease that is thought to be an autoimmune disease against melanocytes. These results suggest that KU-MEL-1 may be a useful target for the development of diagnostic and therapeutic methods for patients with various cancers, particularly with melanoma as well as patients with autoimmune diseases against melanocytes.

Original languageEnglish
Pages (from-to)7900-7907
Number of pages8
JournalCancer Research
Volume61
Issue number21
Publication statusPublished - 2001 Nov 1

Fingerprint

Vitiligo
Neoplasm Antigens
Melanoma
T-Lymphocytes
Melanocytes
Serum
Complementary DNA
Immunoglobulin G
Brain Neoplasms
Cell Line
Colonic Neoplasms
Autoimmune Diseases
Antibodies
Clone Cells
Uveomeningoencephalitic Syndrome
Melanoma-Specific Antigens
Neoplasms
Proteins
Expressed Sequence Tags
Esophageal Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Kiniwa, Y., Fujita, T., Akada, M., Ito, K., Shofuda, T., Matsuzaki, Y., ... Kawakami, Y. (2001). Tumor antigens isolated from a patient with vitiligo and T-cell-infiltrated melanoma. Cancer Research, 61(21), 7900-7907.

Tumor antigens isolated from a patient with vitiligo and T-cell-infiltrated melanoma. / Kiniwa, Y.; Fujita, T.; Akada, M.; Ito, K.; Shofuda, T.; Matsuzaki, Yuriko; Yamamoto, A.; Saida, T.; Kawakami, Yutaka.

In: Cancer Research, Vol. 61, No. 21, 01.11.2001, p. 7900-7907.

Research output: Contribution to journalArticle

Kiniwa, Y, Fujita, T, Akada, M, Ito, K, Shofuda, T, Matsuzaki, Y, Yamamoto, A, Saida, T & Kawakami, Y 2001, 'Tumor antigens isolated from a patient with vitiligo and T-cell-infiltrated melanoma', Cancer Research, vol. 61, no. 21, pp. 7900-7907.
Kiniwa Y, Fujita T, Akada M, Ito K, Shofuda T, Matsuzaki Y et al. Tumor antigens isolated from a patient with vitiligo and T-cell-infiltrated melanoma. Cancer Research. 2001 Nov 1;61(21):7900-7907.
Kiniwa, Y. ; Fujita, T. ; Akada, M. ; Ito, K. ; Shofuda, T. ; Matsuzaki, Yuriko ; Yamamoto, A. ; Saida, T. ; Kawakami, Yutaka. / Tumor antigens isolated from a patient with vitiligo and T-cell-infiltrated melanoma. In: Cancer Research. 2001 ; Vol. 61, No. 21. pp. 7900-7907.
@article{8d853cf75b8c4ccb9211e3909058f943,
title = "Tumor antigens isolated from a patient with vitiligo and T-cell-infiltrated melanoma",
abstract = "Serological identification of tumor antigens by cDNA expression cloning is a technique used to isolate cDNAs encoding tumor antigens that are recognized by IgG antibodies in sera from cancer patients. It is also useful for the isolation of tumor antigens recognized by T cells. We applied this method to identify melanoma antigens recognized by the serum from a patient with a good prognosis who had T-cell-infiltrated melanoma and vitiligo. By screening a λ phage cDNA library constructed from a highly pigmented melanoma cell line, SKme123, with the patient's serum, 50 positive cDNA clones consisting of 26 distinct antigens were isolated. Of these, 20 encoded known proteins, and 6 encoded previously uncharacterized ones. The most frequently isolated clone, which we named KU-MEL-1, was unknown previously but was homologous to partial cDNA sequences registered in the expressed sequence tag database. Reverse transcription-PCR and Northern blot analysis demonstrated that KU-MEL-1 was strongly expressed in most melanoma cell lines, melanoma tissue samples, and cultured melanocytes and weakly expressed in cell lines derived from other types of tumors, as well as in some normal tissues, including testis. Western blot analysis with polyclonal murine antibody generated by immunization with the recombinant KU-MEL-1 protein demonstrated that the KU-MEL-1 protein was preferentially expressed in melanoma cells and melanocytes IgG antibodies against KU-MEL-1 were detected in the sera from 9 of 26 melanoma patients and from some patients with other cancers including brain tumor, esophageal cancer, colon cancer, and chronic myelogenous leukemia, but were not detected in sera from 30 healthy individuals. Although the IgG specific for KU-MEL-1 was not detected in sera from 12 vitiligo patients, it was detected in sera from 7 of 11 patients with Vogt-Koyanagi-Harada disease that is thought to be an autoimmune disease against melanocytes. These results suggest that KU-MEL-1 may be a useful target for the development of diagnostic and therapeutic methods for patients with various cancers, particularly with melanoma as well as patients with autoimmune diseases against melanocytes.",
author = "Y. Kiniwa and T. Fujita and M. Akada and K. Ito and T. Shofuda and Yuriko Matsuzaki and A. Yamamoto and T. Saida and Yutaka Kawakami",
year = "2001",
month = "11",
day = "1",
language = "English",
volume = "61",
pages = "7900--7907",
journal = "Cancer Research",
issn = "0008-5472",
publisher = "American Association for Cancer Research Inc.",
number = "21",

}

TY - JOUR

T1 - Tumor antigens isolated from a patient with vitiligo and T-cell-infiltrated melanoma

AU - Kiniwa, Y.

AU - Fujita, T.

AU - Akada, M.

AU - Ito, K.

AU - Shofuda, T.

AU - Matsuzaki, Yuriko

AU - Yamamoto, A.

AU - Saida, T.

AU - Kawakami, Yutaka

PY - 2001/11/1

Y1 - 2001/11/1

N2 - Serological identification of tumor antigens by cDNA expression cloning is a technique used to isolate cDNAs encoding tumor antigens that are recognized by IgG antibodies in sera from cancer patients. It is also useful for the isolation of tumor antigens recognized by T cells. We applied this method to identify melanoma antigens recognized by the serum from a patient with a good prognosis who had T-cell-infiltrated melanoma and vitiligo. By screening a λ phage cDNA library constructed from a highly pigmented melanoma cell line, SKme123, with the patient's serum, 50 positive cDNA clones consisting of 26 distinct antigens were isolated. Of these, 20 encoded known proteins, and 6 encoded previously uncharacterized ones. The most frequently isolated clone, which we named KU-MEL-1, was unknown previously but was homologous to partial cDNA sequences registered in the expressed sequence tag database. Reverse transcription-PCR and Northern blot analysis demonstrated that KU-MEL-1 was strongly expressed in most melanoma cell lines, melanoma tissue samples, and cultured melanocytes and weakly expressed in cell lines derived from other types of tumors, as well as in some normal tissues, including testis. Western blot analysis with polyclonal murine antibody generated by immunization with the recombinant KU-MEL-1 protein demonstrated that the KU-MEL-1 protein was preferentially expressed in melanoma cells and melanocytes IgG antibodies against KU-MEL-1 were detected in the sera from 9 of 26 melanoma patients and from some patients with other cancers including brain tumor, esophageal cancer, colon cancer, and chronic myelogenous leukemia, but were not detected in sera from 30 healthy individuals. Although the IgG specific for KU-MEL-1 was not detected in sera from 12 vitiligo patients, it was detected in sera from 7 of 11 patients with Vogt-Koyanagi-Harada disease that is thought to be an autoimmune disease against melanocytes. These results suggest that KU-MEL-1 may be a useful target for the development of diagnostic and therapeutic methods for patients with various cancers, particularly with melanoma as well as patients with autoimmune diseases against melanocytes.

AB - Serological identification of tumor antigens by cDNA expression cloning is a technique used to isolate cDNAs encoding tumor antigens that are recognized by IgG antibodies in sera from cancer patients. It is also useful for the isolation of tumor antigens recognized by T cells. We applied this method to identify melanoma antigens recognized by the serum from a patient with a good prognosis who had T-cell-infiltrated melanoma and vitiligo. By screening a λ phage cDNA library constructed from a highly pigmented melanoma cell line, SKme123, with the patient's serum, 50 positive cDNA clones consisting of 26 distinct antigens were isolated. Of these, 20 encoded known proteins, and 6 encoded previously uncharacterized ones. The most frequently isolated clone, which we named KU-MEL-1, was unknown previously but was homologous to partial cDNA sequences registered in the expressed sequence tag database. Reverse transcription-PCR and Northern blot analysis demonstrated that KU-MEL-1 was strongly expressed in most melanoma cell lines, melanoma tissue samples, and cultured melanocytes and weakly expressed in cell lines derived from other types of tumors, as well as in some normal tissues, including testis. Western blot analysis with polyclonal murine antibody generated by immunization with the recombinant KU-MEL-1 protein demonstrated that the KU-MEL-1 protein was preferentially expressed in melanoma cells and melanocytes IgG antibodies against KU-MEL-1 were detected in the sera from 9 of 26 melanoma patients and from some patients with other cancers including brain tumor, esophageal cancer, colon cancer, and chronic myelogenous leukemia, but were not detected in sera from 30 healthy individuals. Although the IgG specific for KU-MEL-1 was not detected in sera from 12 vitiligo patients, it was detected in sera from 7 of 11 patients with Vogt-Koyanagi-Harada disease that is thought to be an autoimmune disease against melanocytes. These results suggest that KU-MEL-1 may be a useful target for the development of diagnostic and therapeutic methods for patients with various cancers, particularly with melanoma as well as patients with autoimmune diseases against melanocytes.

UR - http://www.scopus.com/inward/record.url?scp=0035503009&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035503009&partnerID=8YFLogxK

M3 - Article

VL - 61

SP - 7900

EP - 7907

JO - Cancer Research

JF - Cancer Research

SN - 0008-5472

IS - 21

ER -