Tumor growth and metastasis of human colorectal cancer cell lines in SCID mice resemble clinical metastatic behaviors

Nobutaka Yasui, Michiie Sakamoto, Atsushi Ochiai, Yoshinori Ino, Shingo Akimoto, Atsushi Orikasa, Masaki Kitajima, Setsuo Hirohashi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Ten human colorectal cancer (CRC) cell lines were implanted orthotopically into the ceca and also into the livers, muscles and peritoneal cavities of SCID mice in order to analyze the characteristics regulating metastatic behaviors of CRCs. All the CRC cell lines formed tumors in the muscle and cecum, but they could be classified into two groups: (1) six cell lines with high tumorigenicity in the liver (HTLs) forming differentiated tumors, and (2) four with no tumorigenicity in the liver (NTLs) forming poorly differentiated tumors in SCID mice. After orthotopic implantation, NTLs never metastasized to the liver, whereas HTLs did. Therefore, intrahepatic tumorigenicity and differential status were closely associated with liver metastasis whereas differentiation was not associated with lung metastasis. The 6 HTLs demonstrated an inverse correlation between liver metastases and peritoneal dissemination, and immunohistochemistry indicated expression of sLeX, CA19-9 and carcinoembryonic antigen in tumors which correlated well with the liver metastatic rate. We found a strong correlation between liver metastasis and intrahepatic tumorigenicity and could reproduce the clinical correlations between the pattern of the metastatic spread and the differentiation phenotype of CRC in vivo. We consider further examination using this model will be useful for analyzing the complex mechanisms involved in clinically metastasizing CRCs.

Original languageEnglish
Pages (from-to)259-269
Number of pages11
JournalInvasion and Metastasis
Volume17
Issue number5
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

SCID Mice
Colorectal Neoplasms
Neoplasm Metastasis
Cell Line
Liver
Growth
Neoplasms
Cecum
Muscles
Carcinoembryonic Antigen
Peritoneal Cavity
Tumor Cell Line
Immunohistochemistry
Phenotype

Keywords

  • Colorectal cancer
  • Metastasis
  • SCID mice

ASJC Scopus subject areas

  • Cancer Research

Cite this

Yasui, N., Sakamoto, M., Ochiai, A., Ino, Y., Akimoto, S., Orikasa, A., ... Hirohashi, S. (1997). Tumor growth and metastasis of human colorectal cancer cell lines in SCID mice resemble clinical metastatic behaviors. Invasion and Metastasis, 17(5), 259-269.

Tumor growth and metastasis of human colorectal cancer cell lines in SCID mice resemble clinical metastatic behaviors. / Yasui, Nobutaka; Sakamoto, Michiie; Ochiai, Atsushi; Ino, Yoshinori; Akimoto, Shingo; Orikasa, Atsushi; Kitajima, Masaki; Hirohashi, Setsuo.

In: Invasion and Metastasis, Vol. 17, No. 5, 1997, p. 259-269.

Research output: Contribution to journalArticle

Yasui, N, Sakamoto, M, Ochiai, A, Ino, Y, Akimoto, S, Orikasa, A, Kitajima, M & Hirohashi, S 1997, 'Tumor growth and metastasis of human colorectal cancer cell lines in SCID mice resemble clinical metastatic behaviors', Invasion and Metastasis, vol. 17, no. 5, pp. 259-269.
Yasui, Nobutaka ; Sakamoto, Michiie ; Ochiai, Atsushi ; Ino, Yoshinori ; Akimoto, Shingo ; Orikasa, Atsushi ; Kitajima, Masaki ; Hirohashi, Setsuo. / Tumor growth and metastasis of human colorectal cancer cell lines in SCID mice resemble clinical metastatic behaviors. In: Invasion and Metastasis. 1997 ; Vol. 17, No. 5. pp. 259-269.
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