Abstract
Epithelial-mesenchymal transition (EMT) is a series of events during which epithelial cells lose many of their epithelial characteristics and take on properties that are typical of mesenchymal cells that lack cell-cell adhesion properties. EMT may be activated by various types of growth factors or inflammatory cytokines. In many types of epithelial cancers, the EMT-derived tumor cells are susceptible to metastasis. During tumor progression, epithelial cells acquire a gene expression pattern closely resembling that of mesenchymal cells. This study aimed to investigate the expression of the EMT-associated transcription factor Snail and an adhesion molecule E-cadherin in cholangiocarcinoma (CCA) tissues. The effect of TNF-α on EMT activation in CCA cells was also demonstrated. The qRT-PCR analysis revealed that Snail expression significantly increased in CCA (P = 0.01) and was correlated with tumor metastasis (P = 0.02). The expression of Snail was inversely associated with E-cadherin (P = 0.004). The stimulation of TNF-α enhances migration behavior and showed significantly induced expression of Snail in CCA cell lines, whereas expression of E-cadherin and CK-19 (the epithelial marker) was reduced. Immunofluorescence analysis revealed that TNF-α-treated CCA cell lines increased nuclear translocation of Snail, whereas E-cadherin was dramatically decreased. Our findings suggest that the changes in the expression of Snail or E-cadherin might regulate EMT development in CCA resulting in promoting tumor progression. Overexpression of Snail could be used as a prognostic marker for monitoring the treatment efficiency of CCA patients.
| Original language | English |
|---|---|
| Pages (from-to) | 3083-3091 |
| Number of pages | 9 |
| Journal | Medical Oncology |
| Volume | 29 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2012 Dec |
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Keywords
- Cholangiocarcinoma
- E-cadherin
- Epithelial-mesenchymal transition
- Snail
- TNF-α
ASJC Scopus subject areas
- Oncology
- Cancer Research
- Hematology
- Medicine(all)
Cite this
Tumor necrosis factor-α (TNF-α) stimulates the epithelial-mesenchymal transition regulator Snail in cholangiocarcinoma. / Techasen, Anchalee; Namwat, Nisana; Loilome, Watcharin; Bungkanjana, Pornpan; Khuntikeo, Narong; Puapairoj, Anucha; Jearanaikoon, Patcharee; Saya, Hideyuki; Yongvanit, Puangrat.
In: Medical Oncology, Vol. 29, No. 5, 12.2012, p. 3083-3091.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Tumor necrosis factor-α (TNF-α) stimulates the epithelial-mesenchymal transition regulator Snail in cholangiocarcinoma
AU - Techasen, Anchalee
AU - Namwat, Nisana
AU - Loilome, Watcharin
AU - Bungkanjana, Pornpan
AU - Khuntikeo, Narong
AU - Puapairoj, Anucha
AU - Jearanaikoon, Patcharee
AU - Saya, Hideyuki
AU - Yongvanit, Puangrat
PY - 2012/12
Y1 - 2012/12
N2 - Epithelial-mesenchymal transition (EMT) is a series of events during which epithelial cells lose many of their epithelial characteristics and take on properties that are typical of mesenchymal cells that lack cell-cell adhesion properties. EMT may be activated by various types of growth factors or inflammatory cytokines. In many types of epithelial cancers, the EMT-derived tumor cells are susceptible to metastasis. During tumor progression, epithelial cells acquire a gene expression pattern closely resembling that of mesenchymal cells. This study aimed to investigate the expression of the EMT-associated transcription factor Snail and an adhesion molecule E-cadherin in cholangiocarcinoma (CCA) tissues. The effect of TNF-α on EMT activation in CCA cells was also demonstrated. The qRT-PCR analysis revealed that Snail expression significantly increased in CCA (P = 0.01) and was correlated with tumor metastasis (P = 0.02). The expression of Snail was inversely associated with E-cadherin (P = 0.004). The stimulation of TNF-α enhances migration behavior and showed significantly induced expression of Snail in CCA cell lines, whereas expression of E-cadherin and CK-19 (the epithelial marker) was reduced. Immunofluorescence analysis revealed that TNF-α-treated CCA cell lines increased nuclear translocation of Snail, whereas E-cadherin was dramatically decreased. Our findings suggest that the changes in the expression of Snail or E-cadherin might regulate EMT development in CCA resulting in promoting tumor progression. Overexpression of Snail could be used as a prognostic marker for monitoring the treatment efficiency of CCA patients.
AB - Epithelial-mesenchymal transition (EMT) is a series of events during which epithelial cells lose many of their epithelial characteristics and take on properties that are typical of mesenchymal cells that lack cell-cell adhesion properties. EMT may be activated by various types of growth factors or inflammatory cytokines. In many types of epithelial cancers, the EMT-derived tumor cells are susceptible to metastasis. During tumor progression, epithelial cells acquire a gene expression pattern closely resembling that of mesenchymal cells. This study aimed to investigate the expression of the EMT-associated transcription factor Snail and an adhesion molecule E-cadherin in cholangiocarcinoma (CCA) tissues. The effect of TNF-α on EMT activation in CCA cells was also demonstrated. The qRT-PCR analysis revealed that Snail expression significantly increased in CCA (P = 0.01) and was correlated with tumor metastasis (P = 0.02). The expression of Snail was inversely associated with E-cadherin (P = 0.004). The stimulation of TNF-α enhances migration behavior and showed significantly induced expression of Snail in CCA cell lines, whereas expression of E-cadherin and CK-19 (the epithelial marker) was reduced. Immunofluorescence analysis revealed that TNF-α-treated CCA cell lines increased nuclear translocation of Snail, whereas E-cadherin was dramatically decreased. Our findings suggest that the changes in the expression of Snail or E-cadherin might regulate EMT development in CCA resulting in promoting tumor progression. Overexpression of Snail could be used as a prognostic marker for monitoring the treatment efficiency of CCA patients.
KW - Cholangiocarcinoma
KW - E-cadherin
KW - Epithelial-mesenchymal transition
KW - Snail
KW - TNF-α
UR - http://www.scopus.com/inward/record.url?scp=84880260044&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84880260044&partnerID=8YFLogxK
U2 - 10.1007/s12032-012-0305-x
DO - 10.1007/s12032-012-0305-x
M3 - Article
VL - 29
SP - 3083
EP - 3091
JO - Medical Oncology
JF - Medical Oncology
SN - 1357-0560
IS - 5
ER -