Tumor necrosis factor-alpha-mutant mice exhibit high frequency hearing loss

Naoki Oishi, Jun Chen, Hong Wei Zheng, Kayla Hill, Jochen Schacht, Su Hua Sha

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Exogenous tumor necrosis factor-alpha (TNF-α) plays a role in auditory hair cell death by altering the expression of apoptosis-related genes in response to noxious stimuli. Little is known, however, about the function of TNF-α in normal hair cell physiology. We, therefore, investigated the cochlear morphology and auditory function of TNF-α-deficient mice. Auditory evoked brainstem response showed significantly higher thresholds, especially at higher frequencies, in 1-month-old TNF-α-/- mice as compared to TNF-α+/- and wild type (WT); hearing loss did not progress further from 1 to 4 months of age. There was no difference in the gross morphology of the organ of Corti, lateral wall, and spiral ganglion cells in TNF-α-/- mice compared to WT mice at 4 months of age, nor were there differences in the anatomy of the auditory ossicles. Outer hair cells were completely intact in surface preparations of the organ of Corti of TNF-α-/- mice, and synaptic ribbon counts of TNF-α-/- and WT mice at 4 months of age were similar. Reduced amplitudes of distortion product otoacoustic emissions, however, indicated dysfunction of outer hair cells in TNF-α-/- mice. Scanning electron microscopy revealed that stereocilia were sporadically absent in the basal turn and distorted in the middle turn. In summary, our results demonstrate that TNF-α-mutant mice exhibit early hearing loss, especially at higher frequencies, and that loss or malformation of the stereocilia of outer hair cells appears to be a contributing factor.

Original languageEnglish
Pages (from-to)801-811
Number of pages11
JournalJARO - Journal of the Association for Research in Otolaryngology
Volume14
Issue number6
DOIs
Publication statusPublished - 2013 Dec
Externally publishedYes

Fingerprint

High-Frequency Hearing Loss
Tumor Necrosis Factor-alpha
Outer Auditory Hair Cells
Stereocilia
Organ of Corti
Hearing Loss
Auditory Hair Cells
Ear Ossicles
Spiral Ganglion
Cell Physiological Phenomena
Brain Stem Auditory Evoked Potentials
Cochlea
Electron Scanning Microscopy
Anatomy
Cell Death

Keywords

  • higher frequency hearing loss
  • malformation of the stereocilia of outer hair cells
  • TNF-alpha-deficient mice

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Sensory Systems

Cite this

Tumor necrosis factor-alpha-mutant mice exhibit high frequency hearing loss. / Oishi, Naoki; Chen, Jun; Zheng, Hong Wei; Hill, Kayla; Schacht, Jochen; Sha, Su Hua.

In: JARO - Journal of the Association for Research in Otolaryngology, Vol. 14, No. 6, 12.2013, p. 801-811.

Research output: Contribution to journalArticle

Oishi, Naoki ; Chen, Jun ; Zheng, Hong Wei ; Hill, Kayla ; Schacht, Jochen ; Sha, Su Hua. / Tumor necrosis factor-alpha-mutant mice exhibit high frequency hearing loss. In: JARO - Journal of the Association for Research in Otolaryngology. 2013 ; Vol. 14, No. 6. pp. 801-811.
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