Tumor Suppressor CYLD Regulates JNK-Induced Cell Death in Drosophila

Lei Xue, Tatsushi Igaki, Erina Kuranaga, Hiroshi Kanda, Masayuki Miura, Tian Xu

Research output: Contribution to journalArticlepeer-review

82 Citations (Scopus)

Abstract

CYLD encodes a tumor suppressor that is mutated in familial cylindromatosis. Despite biochemical and cell culture studies, the physiological functions of CYLD in animal development and tumorigenesis remain poorly understood. To address these questions, we generated Drosophila CYLD (dCYLD) mutant and transgenic flies expressing wild-type and mutant dCYLD proteins. Here we show that dCYLD is essential for JNK-dependent oxidative stress resistance and normal lifespan. Furthermore, dCYLD regulates TNF-induced JNK activation and cell death through dTRAF2, which acts downstream of the TNF receptor Wengen and upstream of the JNKK kinase dTAK1. We show that dCYLD encodes a deubiquitinating enzyme that deubiquitinates dTRAF2 and prevents dTRAF2 from ubiquitin-mediated proteolytic degradation. These data provide a molecular mechanism for the tumor suppressor function of this evolutionary conserved molecule by indicating that dCYLD plays a critical role in modulating TNF-JNK-mediated cell death.

Original languageEnglish
Pages (from-to)446-454
Number of pages9
JournalDevelopmental Cell
Volume13
Issue number3
DOIs
Publication statusPublished - 2007 Sep 4

Keywords

  • DEVBIO

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Tumor Suppressor CYLD Regulates JNK-Induced Cell Death in Drosophila'. Together they form a unique fingerprint.

Cite this