Turmeric and curcumin modulate the conjugation of 1-naphthol in caco-2 cells

Megumi Naganuma, Ayako Saruwatari, Shigeaki Okamura, Hiroomi Tamura

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Turmeric, the powdered dry rhizome of the Curcuma longa plant, and curcumin, the major anti-oxidant constituent of turmeric, have been shown to possess chemopreventive activity. To elucidate the possible interaction of turmeric and curcumin with conjugation reactions, which in many cases are involved in the activation of procarcinogens, we measured their effects in the conjugation of 1-naphthol in Caco-2 cells, a human colon carcinoma cell line, within a 24 h period. Turmeric exhibits inhibitory activity toward both sulfo- and glucuronosyl conjugations of 1-naphthol at approximately the same levels (IC50=0.24 and 0.29 mg/ml, respectively). Curcumin inhibits sulfo-conjugation at lower concentrations (IC50=9.7 μg/ml), but only showed weak inhibition toward glucuronosyl conjugation of 1-naphthol in Caco-2 cells. In addition, turmeric was found to strongly inhibit in vitro phenol sulfotransferase (SULT) activity and demonstrate moderate inhibitory properties against UDP-glucuronosyl transferase (UGT) activity in Caco-2 cells (IC50=0.17 mg/ml and 0.62 mg/ml, respectively). Curcumin also strongly inhibits in vitro phenol sulfotransferase activity with an IC 50 of 2.4 μg/ml. Moreover, and in contrast to the moderate inhibition of UGT activity by turmeric and curcumin, both induce the expression of the UGT1A1 and UGT1A6 genes, revealed by real-time PCR analysis. These findings are indicative of a possible interaction of both turmeric and curcumin with conjugation reactions in the human intestinal tract and colon. This in turn may affect the bioavailability of therapeutic drugs and toxicity levels of environmental chemicals, particularly procarcinogens.

Original languageEnglish
Pages (from-to)1476-1479
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume29
Issue number7
DOIs
Publication statusPublished - 2006

Fingerprint

Curcuma
Curcumin
Caco-2 Cells
Arylsulfotransferase
Inhibitory Concentration 50
Uridine Diphosphate
Transferases
Colon
Rhizome
1-naphthol
Drug-Related Side Effects and Adverse Reactions
Oxidants
Biological Availability
Real-Time Polymerase Chain Reaction
Carcinoma
Cell Line

Keywords

  • Caco-2
  • Conjugation
  • Curcumin
  • Sulfotransferase (SULT)
  • Turmeric
  • UDP-glucuronosyl transferase (UGT)

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Turmeric and curcumin modulate the conjugation of 1-naphthol in caco-2 cells. / Naganuma, Megumi; Saruwatari, Ayako; Okamura, Shigeaki; Tamura, Hiroomi.

In: Biological and Pharmaceutical Bulletin, Vol. 29, No. 7, 2006, p. 1476-1479.

Research output: Contribution to journalArticle

Naganuma, Megumi ; Saruwatari, Ayako ; Okamura, Shigeaki ; Tamura, Hiroomi. / Turmeric and curcumin modulate the conjugation of 1-naphthol in caco-2 cells. In: Biological and Pharmaceutical Bulletin. 2006 ; Vol. 29, No. 7. pp. 1476-1479.
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