Two barium binding sites on a maxi K+ channel from human vas deferens epithelial cells

Y. Sohma, A. Harris, C. J C Wardle, B. E. Argent, M. A. Gray

Research output: Contribution to journalArticle

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Abstract

Using the patch clamp technique, we have investigated the blockade of maxi-K+ channels present on vas deferens epithelial cells by extracellular Ba2+. With symmetrical 140 mM K+ solutions, Ba2+ produced discrete blocking events consisting of both long closings of seconds duration (slow block) and fast closings of milliseconds duration (flickering block). Kinetic analysis showed that flickering block occurred according to an 'open channel blocking' schema and was eliminated by reducing external K+ to 4.5 mM. Slow block showed a complex voltage-dependence. At potentials between -20 mV and 20 mV, blockade was voltage-dependent; at potentials greater than 20 mV, blockade was voltage-independent, but markedly sensitive to the extracellular K+ concentration. These data reveal that the vas deferens maxi-K+ channel has two Ba2+ binding sites accessible from the extracellular side. Site one is located at the cytoplasmic side of the gating region and binding to this site causes flickering block. Site two is located close to the extracellular mouth of the channel and binding to this site causes slow block.

Original languageEnglish
Pages (from-to)1316-1325
Number of pages10
JournalBiophysical Journal
Volume70
Issue number3
Publication statusPublished - 1996 Mar
Externally publishedYes

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Large-Conductance Calcium-Activated Potassium Channels
Vas Deferens
Barium
Epithelial Cells
Binding Sites
Patch-Clamp Techniques
Mouth

ASJC Scopus subject areas

  • Biophysics

Cite this

Sohma, Y., Harris, A., Wardle, C. J. C., Argent, B. E., & Gray, M. A. (1996). Two barium binding sites on a maxi K+ channel from human vas deferens epithelial cells. Biophysical Journal, 70(3), 1316-1325.

Two barium binding sites on a maxi K+ channel from human vas deferens epithelial cells. / Sohma, Y.; Harris, A.; Wardle, C. J C; Argent, B. E.; Gray, M. A.

In: Biophysical Journal, Vol. 70, No. 3, 03.1996, p. 1316-1325.

Research output: Contribution to journalArticle

Sohma, Y, Harris, A, Wardle, CJC, Argent, BE & Gray, MA 1996, 'Two barium binding sites on a maxi K+ channel from human vas deferens epithelial cells', Biophysical Journal, vol. 70, no. 3, pp. 1316-1325.
Sohma Y, Harris A, Wardle CJC, Argent BE, Gray MA. Two barium binding sites on a maxi K+ channel from human vas deferens epithelial cells. Biophysical Journal. 1996 Mar;70(3):1316-1325.
Sohma, Y. ; Harris, A. ; Wardle, C. J C ; Argent, B. E. ; Gray, M. A. / Two barium binding sites on a maxi K+ channel from human vas deferens epithelial cells. In: Biophysical Journal. 1996 ; Vol. 70, No. 3. pp. 1316-1325.
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