Two different murine monoclonal antibodies B2 and H2 which are specific for human hepatocytes

Toshio Morizane, Kanji Tsuchimoto, Akiko Iino, Shigeru Matsumura, Hidetsugu Saito, Itsurou Satoh, Naoki Kumagai, Yasutaka Inagaiu, Masaharu Tsuchiya, Thomas Brodin, Hans Olov Sjögren

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Abstract

Two different monoclonal antibodies (McAb) designated H2 and B2 which were specific for human hepatocytes were developed by fusing P3-NS1 with spleen or lymphnode cells from BALB/c mice sensitized by a fractionated soluble phase of a human liver homogenate. It was demonstrated by immunoperoxidase technique that the reactivities of these McAb were restricted to human hepatocytes. Twenty four other organs, rat, rabbit livers were negative in staining. The cytoplasm of hepatocytes was stained by the both McAb although the cell membrane was also stained by H2 McAb. These results suggest that the antigens defined by these are different from LSP which is known to be species-nonspecific and localized exclusively on cell membrane and other liver-specific antigens already known. It is proposed that the antigen defined by H2 McAb is designated human liver-specific antigen 1 (HLSA1) and that the antigen defined by B2 McAb is designated human liver-specific antigen 2 (HLSA2).

Original languageEnglish
Pages (from-to)726-735
Number of pages10
JournalKanzo
Volume27
Issue number6
DOIs
Publication statusPublished - 1986

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Hepatocytes
Monoclonal Antibodies
Antigens
Liver
H-2 Antigens
Negative Staining
Immunoenzyme Techniques
Cytoplasm
Spleen
Cell Membrane
Rabbits

ASJC Scopus subject areas

  • Hepatology

Cite this

Morizane, T., Tsuchimoto, K., Iino, A., Matsumura, S., Saito, H., Satoh, I., ... Sjögren, H. O. (1986). Two different murine monoclonal antibodies B2 and H2 which are specific for human hepatocytes. Kanzo, 27(6), 726-735. https://doi.org/10.2957/kanzo.27.726

Two different murine monoclonal antibodies B2 and H2 which are specific for human hepatocytes. / Morizane, Toshio; Tsuchimoto, Kanji; Iino, Akiko; Matsumura, Shigeru; Saito, Hidetsugu; Satoh, Itsurou; Kumagai, Naoki; Inagaiu, Yasutaka; Tsuchiya, Masaharu; Brodin, Thomas; Sjögren, Hans Olov.

In: Kanzo, Vol. 27, No. 6, 1986, p. 726-735.

Research output: Contribution to journalArticle

Morizane, T, Tsuchimoto, K, Iino, A, Matsumura, S, Saito, H, Satoh, I, Kumagai, N, Inagaiu, Y, Tsuchiya, M, Brodin, T & Sjögren, HO 1986, 'Two different murine monoclonal antibodies B2 and H2 which are specific for human hepatocytes', Kanzo, vol. 27, no. 6, pp. 726-735. https://doi.org/10.2957/kanzo.27.726
Morizane, Toshio ; Tsuchimoto, Kanji ; Iino, Akiko ; Matsumura, Shigeru ; Saito, Hidetsugu ; Satoh, Itsurou ; Kumagai, Naoki ; Inagaiu, Yasutaka ; Tsuchiya, Masaharu ; Brodin, Thomas ; Sjögren, Hans Olov. / Two different murine monoclonal antibodies B2 and H2 which are specific for human hepatocytes. In: Kanzo. 1986 ; Vol. 27, No. 6. pp. 726-735.
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AU - Matsumura, Shigeru

AU - Saito, Hidetsugu

AU - Satoh, Itsurou

AU - Kumagai, Naoki

AU - Inagaiu, Yasutaka

AU - Tsuchiya, Masaharu

AU - Brodin, Thomas

AU - Sjögren, Hans Olov

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