Two-dimensional molecular packing of proteins

H. Sasabe, Taiji Furuno, J. Otomo, H. Tomioka, Y. Urabe, T. Nagamune, K. H. Kim, K. Kobayashi, Y. Kobayashi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A technique involving the physical and/or chemical adsorption of molecules in the subphase to the interfacial Langmuir monolayer has been developed to arrange protein molecules such as ferritin, bacteriorhodopsin (BR) (purple membrane) and cytochrome b562 in closely-packed two dimensional arrays. For better packing of molecules, site-directed mutagenesis of cytochrome b562 was introduced to enhance the hydrophobicity or to stabilize the molecules by introducing a cystine linkage. The photoreaction cycle of BR was analysed. From the sequence analysis of newly isolated BR-like pigments, it was suggested that several amino acid residues around the retinal were conserved and formed a "retinal pocket".

Original languageEnglish
Pages (from-to)99-104
Number of pages6
JournalThin Solid Films
Volume216
Issue number1
DOIs
Publication statusPublished - 1992 Aug 28
Externally publishedYes

Fingerprint

Bacteriorhodopsins
proteins
Proteins
Molecules
cytochromes
Cytochromes
molecules
Cystines
mutagenesis
Mutagenesis
Cystine
monomolecular films
Ferritins
Hydrophobicity
hydrophobicity
pigments
Pigments
linkages
amino acids
Amino acids

ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Condensed Matter Physics
  • Surfaces and Interfaces

Cite this

Sasabe, H., Furuno, T., Otomo, J., Tomioka, H., Urabe, Y., Nagamune, T., ... Kobayashi, Y. (1992). Two-dimensional molecular packing of proteins. Thin Solid Films, 216(1), 99-104. https://doi.org/10.1016/0040-6090(92)90877-E

Two-dimensional molecular packing of proteins. / Sasabe, H.; Furuno, Taiji; Otomo, J.; Tomioka, H.; Urabe, Y.; Nagamune, T.; Kim, K. H.; Kobayashi, K.; Kobayashi, Y.

In: Thin Solid Films, Vol. 216, No. 1, 28.08.1992, p. 99-104.

Research output: Contribution to journalArticle

Sasabe, H, Furuno, T, Otomo, J, Tomioka, H, Urabe, Y, Nagamune, T, Kim, KH, Kobayashi, K & Kobayashi, Y 1992, 'Two-dimensional molecular packing of proteins', Thin Solid Films, vol. 216, no. 1, pp. 99-104. https://doi.org/10.1016/0040-6090(92)90877-E
Sasabe H, Furuno T, Otomo J, Tomioka H, Urabe Y, Nagamune T et al. Two-dimensional molecular packing of proteins. Thin Solid Films. 1992 Aug 28;216(1):99-104. https://doi.org/10.1016/0040-6090(92)90877-E
Sasabe, H. ; Furuno, Taiji ; Otomo, J. ; Tomioka, H. ; Urabe, Y. ; Nagamune, T. ; Kim, K. H. ; Kobayashi, K. ; Kobayashi, Y. / Two-dimensional molecular packing of proteins. In: Thin Solid Films. 1992 ; Vol. 216, No. 1. pp. 99-104.
@article{89f01b8521f44636bf84bca67d24c41c,
title = "Two-dimensional molecular packing of proteins",
abstract = "A technique involving the physical and/or chemical adsorption of molecules in the subphase to the interfacial Langmuir monolayer has been developed to arrange protein molecules such as ferritin, bacteriorhodopsin (BR) (purple membrane) and cytochrome b562 in closely-packed two dimensional arrays. For better packing of molecules, site-directed mutagenesis of cytochrome b562 was introduced to enhance the hydrophobicity or to stabilize the molecules by introducing a cystine linkage. The photoreaction cycle of BR was analysed. From the sequence analysis of newly isolated BR-like pigments, it was suggested that several amino acid residues around the retinal were conserved and formed a {"}retinal pocket{"}.",
author = "H. Sasabe and Taiji Furuno and J. Otomo and H. Tomioka and Y. Urabe and T. Nagamune and Kim, {K. H.} and K. Kobayashi and Y. Kobayashi",
year = "1992",
month = "8",
day = "28",
doi = "10.1016/0040-6090(92)90877-E",
language = "English",
volume = "216",
pages = "99--104",
journal = "Thin Solid Films",
issn = "0040-6090",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Two-dimensional molecular packing of proteins

AU - Sasabe, H.

AU - Furuno, Taiji

AU - Otomo, J.

AU - Tomioka, H.

AU - Urabe, Y.

AU - Nagamune, T.

AU - Kim, K. H.

AU - Kobayashi, K.

AU - Kobayashi, Y.

PY - 1992/8/28

Y1 - 1992/8/28

N2 - A technique involving the physical and/or chemical adsorption of molecules in the subphase to the interfacial Langmuir monolayer has been developed to arrange protein molecules such as ferritin, bacteriorhodopsin (BR) (purple membrane) and cytochrome b562 in closely-packed two dimensional arrays. For better packing of molecules, site-directed mutagenesis of cytochrome b562 was introduced to enhance the hydrophobicity or to stabilize the molecules by introducing a cystine linkage. The photoreaction cycle of BR was analysed. From the sequence analysis of newly isolated BR-like pigments, it was suggested that several amino acid residues around the retinal were conserved and formed a "retinal pocket".

AB - A technique involving the physical and/or chemical adsorption of molecules in the subphase to the interfacial Langmuir monolayer has been developed to arrange protein molecules such as ferritin, bacteriorhodopsin (BR) (purple membrane) and cytochrome b562 in closely-packed two dimensional arrays. For better packing of molecules, site-directed mutagenesis of cytochrome b562 was introduced to enhance the hydrophobicity or to stabilize the molecules by introducing a cystine linkage. The photoreaction cycle of BR was analysed. From the sequence analysis of newly isolated BR-like pigments, it was suggested that several amino acid residues around the retinal were conserved and formed a "retinal pocket".

UR - http://www.scopus.com/inward/record.url?scp=0343462026&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0343462026&partnerID=8YFLogxK

U2 - 10.1016/0040-6090(92)90877-E

DO - 10.1016/0040-6090(92)90877-E

M3 - Article

AN - SCOPUS:0343462026

VL - 216

SP - 99

EP - 104

JO - Thin Solid Films

JF - Thin Solid Films

SN - 0040-6090

IS - 1

ER -