Two dimensional sequential cell assembly by sliding a parylene filter

K. Inoue, K. Kamiya, Yuta Abe, T. Osaki, Norihisa Miki, S. Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We demonstrated the two-dimensional assembly of cell clusters in a plane by sliding parylene filter with cell-trapping sites. Gently trapping cells in a designated order while maintaining the contact between them is necessary to investigate cell-cell interactions. Therefore, we developed the mechanical cell trapping system using a parylene filter which was slid to sequentially trap cells in a straight line and control the order. In this study, by using the cell-trapping system, we succeeded in assembling the two-dimensional cell clusters with a cross-shape and an elephant-shape. This system is readily applicable to analysis cell-cell interaction in biological tissue.

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages508-509
Number of pages2
ISBN (Electronic)9780979806483
Publication statusPublished - 2015
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: 2015 Oct 252015 Oct 29

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period15/10/2515/10/29

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Keywords

  • Biological tissue
  • Cell
  • Membrane protein
  • Microfluidic device
  • Photolithography

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Inoue, K., Kamiya, K., Abe, Y., Osaki, T., Miki, N., & Takeuchi, S. (2015). Two dimensional sequential cell assembly by sliding a parylene filter. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 508-509). Chemical and Biological Microsystems Society.