Two extracellular sialidases from Bifidobacterium bifidum promote the degradation of sialyl-oligosaccharides and support the growth of Bifidobacterium breve

Keita Nishiyama, Aki Nagai, Kazuya Uribayashi, Yuji Yamamoto, Takao Mukai, Nobuhiko Okada

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We investigated the roles of extracellular sialidases (SiaBb1 and SiaBb2) in cross-feeding between sialidase-carrying Bifidobacterium bifidum and sialic acid-utilizing Bifidobacterium breve. Using 6ʹ sialyllactose (6ʹSL) as a carbon source, the number of wild-type B. bifidum cells increased while that of a siabb2-inactivated strain (Δsiabb2) did not. Coculture of these two strains in the presence of 6ʹSL resulted in similar increase in cell numbers. Coculture of wild-type B. bifidum, but not the Δsiabb2 strain, with sialic acid-utilizing Bifidobacterium breve, which cannot release sialic acids from carbohydrates, in the presence of 6ʹSL increased the number of B. breve cells. Moreover, when mucin was used as a carbon source, B. breve growth was increased in cocultures with B. bifidum wild-type and Δsiabb2 strains, suggesting that SiaBb1 may be involved. Additionally, B. breve cell numbers increased during cultivation with recombinant SiaBb1-and SiaBb2-treated mucin as the sole carbon source. These results indicated that B. bifidum SiaBb2 liberated sialic acid from sialyl-human milk oligosaccharides and -mucin glycans, supporting the growth of B. breve through sialic acid cross-feeding. SiaBb1 may assist in the degradation of mucin glycan. Collectively, our results revealed that both the B. bifidum extracellular sialidases promote the utilization of sialylated carbohydrates and supply free sialic acid to other Bifidobacterium strains.

Original languageEnglish
Pages (from-to)22-28
Number of pages7
JournalAnaerobe
Volume52
DOIs
Publication statusPublished - 2018 Aug 1
Externally publishedYes

Fingerprint

Oligosaccharides
N-Acetylneuraminic Acid
Mucins
Growth
Coculture Techniques
Carbon
Polysaccharides
Cell Count
Carbohydrates
Sialic Acids
Bifidobacterium
Neuraminidase
Human Milk
Bifidobacterium breve
Bifidobacterium bifidum
6'-sialyllactose

Keywords

  • Bifidobacterium
  • Colonization
  • Sialic acid
  • Sialidase

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Two extracellular sialidases from Bifidobacterium bifidum promote the degradation of sialyl-oligosaccharides and support the growth of Bifidobacterium breve. / Nishiyama, Keita; Nagai, Aki; Uribayashi, Kazuya; Yamamoto, Yuji; Mukai, Takao; Okada, Nobuhiko.

In: Anaerobe, Vol. 52, 01.08.2018, p. 22-28.

Research output: Contribution to journalArticle

Nishiyama, Keita ; Nagai, Aki ; Uribayashi, Kazuya ; Yamamoto, Yuji ; Mukai, Takao ; Okada, Nobuhiko. / Two extracellular sialidases from Bifidobacterium bifidum promote the degradation of sialyl-oligosaccharides and support the growth of Bifidobacterium breve. In: Anaerobe. 2018 ; Vol. 52. pp. 22-28.
@article{5994b197ff184934bc14c9656ad6c7ca,
title = "Two extracellular sialidases from Bifidobacterium bifidum promote the degradation of sialyl-oligosaccharides and support the growth of Bifidobacterium breve",
abstract = "We investigated the roles of extracellular sialidases (SiaBb1 and SiaBb2) in cross-feeding between sialidase-carrying Bifidobacterium bifidum and sialic acid-utilizing Bifidobacterium breve. Using 6ʹ sialyllactose (6ʹSL) as a carbon source, the number of wild-type B. bifidum cells increased while that of a siabb2-inactivated strain (Δsiabb2) did not. Coculture of these two strains in the presence of 6ʹSL resulted in similar increase in cell numbers. Coculture of wild-type B. bifidum, but not the Δsiabb2 strain, with sialic acid-utilizing Bifidobacterium breve, which cannot release sialic acids from carbohydrates, in the presence of 6ʹSL increased the number of B. breve cells. Moreover, when mucin was used as a carbon source, B. breve growth was increased in cocultures with B. bifidum wild-type and Δsiabb2 strains, suggesting that SiaBb1 may be involved. Additionally, B. breve cell numbers increased during cultivation with recombinant SiaBb1-and SiaBb2-treated mucin as the sole carbon source. These results indicated that B. bifidum SiaBb2 liberated sialic acid from sialyl-human milk oligosaccharides and -mucin glycans, supporting the growth of B. breve through sialic acid cross-feeding. SiaBb1 may assist in the degradation of mucin glycan. Collectively, our results revealed that both the B. bifidum extracellular sialidases promote the utilization of sialylated carbohydrates and supply free sialic acid to other Bifidobacterium strains.",
keywords = "Bifidobacterium, Colonization, Sialic acid, Sialidase",
author = "Keita Nishiyama and Aki Nagai and Kazuya Uribayashi and Yuji Yamamoto and Takao Mukai and Nobuhiko Okada",
year = "2018",
month = "8",
day = "1",
doi = "10.1016/j.anaerobe.2018.05.007",
language = "English",
volume = "52",
pages = "22--28",
journal = "Anaerobe",
issn = "1075-9964",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Two extracellular sialidases from Bifidobacterium bifidum promote the degradation of sialyl-oligosaccharides and support the growth of Bifidobacterium breve

AU - Nishiyama, Keita

AU - Nagai, Aki

AU - Uribayashi, Kazuya

AU - Yamamoto, Yuji

AU - Mukai, Takao

AU - Okada, Nobuhiko

PY - 2018/8/1

Y1 - 2018/8/1

N2 - We investigated the roles of extracellular sialidases (SiaBb1 and SiaBb2) in cross-feeding between sialidase-carrying Bifidobacterium bifidum and sialic acid-utilizing Bifidobacterium breve. Using 6ʹ sialyllactose (6ʹSL) as a carbon source, the number of wild-type B. bifidum cells increased while that of a siabb2-inactivated strain (Δsiabb2) did not. Coculture of these two strains in the presence of 6ʹSL resulted in similar increase in cell numbers. Coculture of wild-type B. bifidum, but not the Δsiabb2 strain, with sialic acid-utilizing Bifidobacterium breve, which cannot release sialic acids from carbohydrates, in the presence of 6ʹSL increased the number of B. breve cells. Moreover, when mucin was used as a carbon source, B. breve growth was increased in cocultures with B. bifidum wild-type and Δsiabb2 strains, suggesting that SiaBb1 may be involved. Additionally, B. breve cell numbers increased during cultivation with recombinant SiaBb1-and SiaBb2-treated mucin as the sole carbon source. These results indicated that B. bifidum SiaBb2 liberated sialic acid from sialyl-human milk oligosaccharides and -mucin glycans, supporting the growth of B. breve through sialic acid cross-feeding. SiaBb1 may assist in the degradation of mucin glycan. Collectively, our results revealed that both the B. bifidum extracellular sialidases promote the utilization of sialylated carbohydrates and supply free sialic acid to other Bifidobacterium strains.

AB - We investigated the roles of extracellular sialidases (SiaBb1 and SiaBb2) in cross-feeding between sialidase-carrying Bifidobacterium bifidum and sialic acid-utilizing Bifidobacterium breve. Using 6ʹ sialyllactose (6ʹSL) as a carbon source, the number of wild-type B. bifidum cells increased while that of a siabb2-inactivated strain (Δsiabb2) did not. Coculture of these two strains in the presence of 6ʹSL resulted in similar increase in cell numbers. Coculture of wild-type B. bifidum, but not the Δsiabb2 strain, with sialic acid-utilizing Bifidobacterium breve, which cannot release sialic acids from carbohydrates, in the presence of 6ʹSL increased the number of B. breve cells. Moreover, when mucin was used as a carbon source, B. breve growth was increased in cocultures with B. bifidum wild-type and Δsiabb2 strains, suggesting that SiaBb1 may be involved. Additionally, B. breve cell numbers increased during cultivation with recombinant SiaBb1-and SiaBb2-treated mucin as the sole carbon source. These results indicated that B. bifidum SiaBb2 liberated sialic acid from sialyl-human milk oligosaccharides and -mucin glycans, supporting the growth of B. breve through sialic acid cross-feeding. SiaBb1 may assist in the degradation of mucin glycan. Collectively, our results revealed that both the B. bifidum extracellular sialidases promote the utilization of sialylated carbohydrates and supply free sialic acid to other Bifidobacterium strains.

KW - Bifidobacterium

KW - Colonization

KW - Sialic acid

KW - Sialidase

UR - http://www.scopus.com/inward/record.url?scp=85049298843&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85049298843&partnerID=8YFLogxK

U2 - 10.1016/j.anaerobe.2018.05.007

DO - 10.1016/j.anaerobe.2018.05.007

M3 - Article

C2 - 29787815

AN - SCOPUS:85049298843

VL - 52

SP - 22

EP - 28

JO - Anaerobe

JF - Anaerobe

SN - 1075-9964

ER -