Two types of Na+/K+-ATPase alpha subunit gene transcript in embryos of the sea urchin, Hemicentrotus pulcherrimus

Ken Yamazaki, Chisato Okamura, Toshio Ihara, Ikuo Yasumasu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We have characterized the nucleotide sequence of Na+/K+-ATPase alpha subunit (NKA) cDNA in embryos of the sea urchin, Hemicentrotus pulcherrimus. The primer extension experiments showed that the sea urchin NKA gene generated multiple lengths of transcript. To obtain the 5′-ends of the transcripts, we isolated cDNA clones by the rapid amplification of cDNA ends (RACE). These clones were classified into 2 types on the basis of their 5′ leader sequences. The sequences of the clones were identical except their 5′ leaders. By Northern blot analysis, 1 of the 2 types of transcripts was always detectable in sea urchin embryos during early development, and another was not detected before the morula stage. Genomic PCR demonstrated that the two 5′ leaders were coded by different exons separated by an intron in a single gene. These results show that the transcripts coding 2 isoforms were expressed from a single gene.

Original languageEnglish
Pages (from-to)469-473
Number of pages5
JournalZoological Science
Volume14
Issue number3
DOIs
Publication statusPublished - 1997 Jun

ASJC Scopus subject areas

  • Animal Science and Zoology

Fingerprint Dive into the research topics of 'Two types of Na<sup>+</sup>/K<sup>+</sup>-ATPase alpha subunit gene transcript in embryos of the sea urchin, Hemicentrotus pulcherrimus'. Together they form a unique fingerprint.

Cite this