Tyk2 signaling in host environment plays an important role in contraction of antigen-specific CD8+ T cells following a microbial infection

Wei Li, Hisakata Yamada, Toshiki Yajima, Ryusuke Nakagawa, Kazuya Shimoda, Keiichi Nakayama, Yasunobu Yoshikai

Research output: Contribution to journalArticle

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Abstract

Tyrosine kinase 2 (Tyk2), a member of JAK signal transducer family contributes to the signals triggered by IL-12 for IFN-γ production. To elucidate potential roles of Tyk2 in generation and maintenance of Ag-specific CD8+ T cells, we followed the fate of OVA-specific CD8+ T cells in Tyk2-deficient (-/-) mice after infection with recombinant Listeria monocytogenes expressing OVA (rLM-OVA). Results showed that the numbers of OVA257-264/Kb tetramer-positive CD8+ T cells in Tyk2-/- mice were almost the same as those in Tyk2+/+ mice at the expansion phase on day 7 but were significantly larger in Tyk2 -/- mice than those in Tyk2+/+ mice at the contraction phase on day 10 and at the memory phase on day 60 after infection. The intracellular expression level of active caspase-3 was significantly decreased in the OVA-specific CD8+ T cells of Tyk2-/- mice on day 7 compared with those of Tyk2+/+ mice. Adaptive transfer experiments revealed that Tyk2 signaling in other factors rather than CD8+ T cells played a regulatory role in CD8+ T cell contraction following infection. Administration of exogenous IFN-γ from day 6 to day 9 restored the CD8+ T cell contraction in Tyk2-/- mice after infection with rLM-OVA. These results suggest that Tyk2 signaling for IFN-γ production in host environment plays an important role in contraction of effector CD8+ T cells following a microbial infection.

Original languageEnglish
Pages (from-to)4482-4488
Number of pages7
JournalJournal of Immunology
Volume178
Issue number7
Publication statusPublished - 2007 Apr 1
Externally publishedYes

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TYK2 Kinase
CD8 Antigens
T-Lymphocytes
Infection
Listeria monocytogenes
Mouse Tyk2 protein
Interleukin-12
Transducers
Caspase 3
Maintenance

ASJC Scopus subject areas

  • Immunology

Cite this

Li, W., Yamada, H., Yajima, T., Nakagawa, R., Shimoda, K., Nakayama, K., & Yoshikai, Y. (2007). Tyk2 signaling in host environment plays an important role in contraction of antigen-specific CD8+ T cells following a microbial infection. Journal of Immunology, 178(7), 4482-4488.

Tyk2 signaling in host environment plays an important role in contraction of antigen-specific CD8+ T cells following a microbial infection. / Li, Wei; Yamada, Hisakata; Yajima, Toshiki; Nakagawa, Ryusuke; Shimoda, Kazuya; Nakayama, Keiichi; Yoshikai, Yasunobu.

In: Journal of Immunology, Vol. 178, No. 7, 01.04.2007, p. 4482-4488.

Research output: Contribution to journalArticle

Li, W, Yamada, H, Yajima, T, Nakagawa, R, Shimoda, K, Nakayama, K & Yoshikai, Y 2007, 'Tyk2 signaling in host environment plays an important role in contraction of antigen-specific CD8+ T cells following a microbial infection', Journal of Immunology, vol. 178, no. 7, pp. 4482-4488.
Li, Wei ; Yamada, Hisakata ; Yajima, Toshiki ; Nakagawa, Ryusuke ; Shimoda, Kazuya ; Nakayama, Keiichi ; Yoshikai, Yasunobu. / Tyk2 signaling in host environment plays an important role in contraction of antigen-specific CD8+ T cells following a microbial infection. In: Journal of Immunology. 2007 ; Vol. 178, No. 7. pp. 4482-4488.
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