Tyrosine phosphorylation and subcellular localization of focal adhesion proteins during in vitro decidualization of human endometrial stromal cells

Tetsuo Maruyama, Yasunori Yoshimura, Hisataka Sabe

Research output: Contribution to journalArticle

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Abstract

Human endometrial stromal cells undergo in vitro decidualization when treated with progesterone and estrogen. Using this model, we previously reported specific changes in the c-Src kinase activity and tyrosine phosphorylation of several proteins during in vitro decidualization. Focal adhesion kinase (FAK) and paxillin are known to form a complex with c-Src at the focal contacts and to participate in the integrin-mediated signal transduction as c-Src substrates. We here examined the tyrosine phosphorylation and subcellular localization of the focal adhesion proteins in stromal cells isolated from human endometrium. We found, however, that the total levels of FAK and paxillin tyrosine phosphorylation were not markedly changed during decidualization or after steroid withdrawal. In our culture system, numerous multicellular nodules were developed in cultures of decidualized stromal cells, within whose nodules the focal contacts were found to disappear. Moreover, disruption of the focal contacts was accompanied by disorganization of the actin-based cytoskeleton. These findings suggest that tyrosine phosphorylation of the endometrial paxillin and FAK is not tightly regulated by the kinase activity of c-Src during in vitro decidualization. The escape from regulation by c-Src may be in part due to the dissociation of the focal adhesion proteins/c-Src complex caused by the breakdown of the focal adhesion plaques as well as the loss of the actin-based cytoskeletal architecture.

Original languageEnglish
Pages (from-to)5982-5990
Number of pages9
JournalEndocrinology
Volume140
Issue number12
Publication statusPublished - 1999

Fingerprint

Focal Adhesions
Stromal Cells
Tyrosine
Phosphorylation
Paxillin
Focal Adhesion Protein-Tyrosine Kinases
Proteins
Endometrium
In Vitro Techniques
Actin Cytoskeleton
Integrins
Progesterone
Actins
Signal Transduction
Estrogens
Steroids

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Tyrosine phosphorylation and subcellular localization of focal adhesion proteins during in vitro decidualization of human endometrial stromal cells. / Maruyama, Tetsuo; Yoshimura, Yasunori; Sabe, Hisataka.

In: Endocrinology, Vol. 140, No. 12, 1999, p. 5982-5990.

Research output: Contribution to journalArticle

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