Ubiquinone-rhodol (UQ-Rh) for fluorescence imaging of NAD(P)H through intracellular activation

Hirokazu Komatsu, Yutaka Shindo, Kotaro Oka, Jonathan P. Hill, Katsuhiko Ariga

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The nicotinamide adenine dinucleotide (NAD) derivatives NADH and NADPH are critical components of cellular energy metabolism and operate as electron carriers. A novel fluorescent ubiquinone-rhodol derivative (UQ-Rh) was developed as a probe for NAD(P)H. By using the artificial promoter [(η5- C5Me5)Ir(phen)(H2O)]2+, intracellular activation and imaging of NAD(P)H were successfully demonstrated. In contrast to bioorthogonal chemistry, this "bioparallel chemistry" approach involves interactions with native biological processes and could potentially be used to control or investigate cellular systems. Active detection: NADH and NADPH are critical components of cellular energy metabolism and operate as electron carriers. A novel fluorescent ubiquinone-rhodol derivative (UQ-Rh) was developed as a probe for NAD(P)H. By using the artificial promoter [(η5-C5Me5)Ir(phen)(H 2O)]2+, intracellular activation and imaging of NAD(P)H were successfully demonstrated.

Original languageEnglish
Pages (from-to)3993-3995
Number of pages3
JournalAngewandte Chemie - International Edition
Volume53
Issue number15
DOIs
Publication statusPublished - 2014 Apr 7

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Keywords

  • NAD(P)H
  • fluorescent probes
  • imaging agents
  • in vivo imaging
  • redox chemistry

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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