Ultraprecision cutting of single-crystal calcium fluoride for fabricating micro flow cell for protein structure analysis

Ryo Komiya, Tetsunari Kimura, Minoru Kubo, Jiwang Yan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In infrared spectroscopic analysis of the structures of protein crystals, a micro-flow cell made of single crystal calcium fluoride (CaF2) is needed. In this research, fly cutting of micro grooves on a CaF2 substrate was conducted using a single-crystal diamond tool. The effects of cutting direction, up/down cut, and tool rake angle on ductile-brittle transition of cutting behavior were investigated. Based on the experimental results and analysis, optimal cutting conditions were selected which enabled ductile machining of CaF2. As a result, a 10 μm deep micro-flow cell was successfully fabricated with surface roughness of 2.4 nmRa. The availability of the flow cell was confirmed by visible absorption spectroscopy of protein.

Original languageEnglish
Title of host publicationProceedings of the 8th International Conference on Leading Edge Manufacturing in 21st Century, LEM 2015
PublisherJapan Society of Mechanical Engineers
Publication statusPublished - 2015 Oct 18
Event8th International Conference on Leading Edge Manufacturing in 21st Century, LEM 2015 - Kyoto, Japan
Duration: 2015 Oct 182015 Oct 22

Other

Other8th International Conference on Leading Edge Manufacturing in 21st Century, LEM 2015
CountryJapan
CityKyoto
Period15/10/1815/10/22

Fingerprint

Calcium fluoride
Single crystals
Proteins
Spectroscopic analysis
Absorption spectroscopy
Diamonds
Machining
Surface roughness
Availability
Infrared radiation
Crystals
Substrates

Keywords

  • Calcium fluoride
  • Ductile machining
  • Flow cell
  • Micro machining
  • Ultraprecision cutting

ASJC Scopus subject areas

  • Industrial and Manufacturing Engineering

Cite this

Komiya, R., Kimura, T., Kubo, M., & Yan, J. (2015). Ultraprecision cutting of single-crystal calcium fluoride for fabricating micro flow cell for protein structure analysis. In Proceedings of the 8th International Conference on Leading Edge Manufacturing in 21st Century, LEM 2015 Japan Society of Mechanical Engineers.

Ultraprecision cutting of single-crystal calcium fluoride for fabricating micro flow cell for protein structure analysis. / Komiya, Ryo; Kimura, Tetsunari; Kubo, Minoru; Yan, Jiwang.

Proceedings of the 8th International Conference on Leading Edge Manufacturing in 21st Century, LEM 2015. Japan Society of Mechanical Engineers, 2015.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Komiya, R, Kimura, T, Kubo, M & Yan, J 2015, Ultraprecision cutting of single-crystal calcium fluoride for fabricating micro flow cell for protein structure analysis. in Proceedings of the 8th International Conference on Leading Edge Manufacturing in 21st Century, LEM 2015. Japan Society of Mechanical Engineers, 8th International Conference on Leading Edge Manufacturing in 21st Century, LEM 2015, Kyoto, Japan, 15/10/18.
Komiya R, Kimura T, Kubo M, Yan J. Ultraprecision cutting of single-crystal calcium fluoride for fabricating micro flow cell for protein structure analysis. In Proceedings of the 8th International Conference on Leading Edge Manufacturing in 21st Century, LEM 2015. Japan Society of Mechanical Engineers. 2015
Komiya, Ryo ; Kimura, Tetsunari ; Kubo, Minoru ; Yan, Jiwang. / Ultraprecision cutting of single-crystal calcium fluoride for fabricating micro flow cell for protein structure analysis. Proceedings of the 8th International Conference on Leading Edge Manufacturing in 21st Century, LEM 2015. Japan Society of Mechanical Engineers, 2015.
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