Ultrasound-induced DNA damage and signal transductions indicated by gammaH2AX

Yukihiro Furusawa, Yoshisada Fujiwara, Qing Li Zhao, Mariame Ali Hassan, Ryohei Ogawa, Yoshiaki Tabuchi, Ichiro Takasaki, Akihisa Takahashi, Takeo Ohnishi, Takashi Kondo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Ultrasound (US) has been shown to induce cancer cell death via different forms including apoptosis. Here, we report the potential of low-intensity pulsed US (LIPUS) to induce genomic DNA damage and subsequent DNA damage response. Using the ionizing radiation-induced DNA doublestrand breaks (DSBs) as the positive control, we were able to observe the induction of DSBs (as neutral comet tails) and the subsequent formation of gammaH2AX-positive foci (by immunofluorescence detection) in human leukemia cells following exposure to LIPUS. The LIPUS-induced DNA damage arose most likely from the mechanical, but not sonochemical, effect of cavitation, based on our observation that the suppression of inertial cavitation abrogated the gammH2AX foci formation, whereas scavenging of free radical formation (e.g., hydroxyl radical) had no protective effect on it. Treatment with the specific kinase inhibitor of ATM or DNA-PKcs, which can phosphorylate H2AX Ser139, revealed that US-induced gammaH2AX was inhibited more effectively by the DNA-PK inhibitor than ATM kinase inhibitor. Notably, these inhibitor effects were opposite to those with radiation-induced gammH2AX. In conclusion, we report, for the first time that US can induce DNA damage and the DNA damage response as indicated by gammaH2AX was triggered by the cavitational mechanical effects. Thus, it is expected that the data shown here may provide a better understanding of the cellular responses to US.

Original languageEnglish
Title of host publicationAIP Conference Proceedings
Pages322-325
Number of pages4
Volume1359
DOIs
Publication statusPublished - 2011
Event10th International Symposium on Therapeutic Ultrasound, ISTU 2010 - Tokyo, Japan
Duration: 2010 Jun 92010 Jun 12

Other

Other10th International Symposium on Therapeutic Ultrasound, ISTU 2010
CountryJapan
CityTokyo
Period10/6/910/6/12

Fingerprint

deoxyribonucleic acid
damage
inhibitors
asynchronous transfer mode
cavitation flow
comet tails
leukemias
scavenging
hydroxyl radicals
apoptosis
death
ionizing radiation
free radicals
induction
cancer
retarding
radiation
cells

Keywords

  • Cavitation
  • DNA damage
  • GammaH2AX
  • Ultrasound

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Furusawa, Y., Fujiwara, Y., Zhao, Q. L., Hassan, M. A., Ogawa, R., Tabuchi, Y., ... Kondo, T. (2011). Ultrasound-induced DNA damage and signal transductions indicated by gammaH2AX. In AIP Conference Proceedings (Vol. 1359, pp. 322-325) https://doi.org/10.1063/1.3607926

Ultrasound-induced DNA damage and signal transductions indicated by gammaH2AX. / Furusawa, Yukihiro; Fujiwara, Yoshisada; Zhao, Qing Li; Hassan, Mariame Ali; Ogawa, Ryohei; Tabuchi, Yoshiaki; Takasaki, Ichiro; Takahashi, Akihisa; Ohnishi, Takeo; Kondo, Takashi.

AIP Conference Proceedings. Vol. 1359 2011. p. 322-325.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Furusawa, Y, Fujiwara, Y, Zhao, QL, Hassan, MA, Ogawa, R, Tabuchi, Y, Takasaki, I, Takahashi, A, Ohnishi, T & Kondo, T 2011, Ultrasound-induced DNA damage and signal transductions indicated by gammaH2AX. in AIP Conference Proceedings. vol. 1359, pp. 322-325, 10th International Symposium on Therapeutic Ultrasound, ISTU 2010, Tokyo, Japan, 10/6/9. https://doi.org/10.1063/1.3607926
Furusawa Y, Fujiwara Y, Zhao QL, Hassan MA, Ogawa R, Tabuchi Y et al. Ultrasound-induced DNA damage and signal transductions indicated by gammaH2AX. In AIP Conference Proceedings. Vol. 1359. 2011. p. 322-325 https://doi.org/10.1063/1.3607926
Furusawa, Yukihiro ; Fujiwara, Yoshisada ; Zhao, Qing Li ; Hassan, Mariame Ali ; Ogawa, Ryohei ; Tabuchi, Yoshiaki ; Takasaki, Ichiro ; Takahashi, Akihisa ; Ohnishi, Takeo ; Kondo, Takashi. / Ultrasound-induced DNA damage and signal transductions indicated by gammaH2AX. AIP Conference Proceedings. Vol. 1359 2011. pp. 322-325
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