Ultrastructural changes in mice actively producing antibodies to desmoglein 3 parallel those in patients with pemphigus vulgaris

Atsushi Shimizu, Akira Ishiko, Takayuki Ota, Kazuyuki Tsunoda, Shigeo Koyasu, Masayuki Amagai, Takeji Nishikawa

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Pemphigus vulgaris (PV) is an autoimmune blistering disease caused by autoantibodies against the desmosomal cadherins, desmogleins 1 and 3 (Dsg1, Dsg3) of which Dsg3 plays a major pathogenic role. We have previously generated a novel active disease mouse model for PV, which was produced by the transfer of splenocytes from Dsg3-/- mice, immunized with recombinant mouse Dsg3, into Rag2-/--immunodeficient mice that express Dsg3. In this study, we undertook a further analysis of these PV model mice using electron microscopy (EM). We compared the ultrastructure of the epithelia of PV model mice with that of Dsg3-/- mice to highlight the mechanisms of blister formation in PV. These PV model mice showed the ultrastructural phenotype of PV, which is characterized by suprabasal acantholysis, rows of tombstone basal keratinocytes and half-desmosomes. Additionally, patchy hair loss was observed in PV model mice as in Dsg3-/- mice, and the ultrastructure of the telogen hair follicles was indistinguishable between PV model mice and Dsg3-/- mice. These results demonstrate that anti-Dsg3 autoantibodies interfere with the cell-cell adhesion of keratinocytes in PV model mice. In conclusion, our model mice closely represent the disease phenotype of PV at the ultrastructural level and can therefore be utilized not only as a clinical disease model but also to study the molecular mechanisms involved in blister formation in PV.

Original languageEnglish
Pages (from-to)318-323
Number of pages6
JournalArchives of Dermatological Research
Volume294
Issue number7
Publication statusPublished - 2002

Fingerprint

Desmoglein 3
Pemphigus
Antibodies
Cadherins
Blister
Keratinocytes
Autoantibodies
Desmosomal Cadherins
Desmoglein 1
Acantholysis
Phenotype
Desmosomes
Hair Follicle
Alopecia

Keywords

  • Desmoglein 3
  • Electron microscopy
  • Experimental model
  • Pemphigus

ASJC Scopus subject areas

  • Dermatology

Cite this

Ultrastructural changes in mice actively producing antibodies to desmoglein 3 parallel those in patients with pemphigus vulgaris. / Shimizu, Atsushi; Ishiko, Akira; Ota, Takayuki; Tsunoda, Kazuyuki; Koyasu, Shigeo; Amagai, Masayuki; Nishikawa, Takeji.

In: Archives of Dermatological Research, Vol. 294, No. 7, 2002, p. 318-323.

Research output: Contribution to journalArticle

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