Ultrastructural characterization of surface-induced platelet activation on artificial materials by transmission electron microscopy

Yukihiro Yoshimoto, Terumitsu Hasebe, Kei Takahashi, Masao Amari, So Nagashima, Aki Kamijo, Atsushi Hotta, Koki Takahashi, Tetsuya Suzuki

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Platelet adhesion is one of the most pivotal events of blood clotting for artificial surfaces. However, the mechanisms of surface-induced platelet activation have not been fully been elucidated or visualized so far. In this study, we attempted to observe the internal structures and adhesion interfaces of human platelets attached to artificial surfaces by transmission electron microscopy (TEM) during the platelet activation process. We prepared observation samples by a conventional embedding method using EPON 812 resin. The sectioning was sliced perpendicular to the a-platelet/material interface. Observation by TEM indicates that internal granules coalesce in the center of the platelet accompanied by pseudopodial growth in the early stage of platelet activation. Pseudopodia from a platelet attach to the material interface not along a plane but at a point. In addition, along with the process of platelet activation, the gap between the platelet membrane and the material surface at the interface disappeared and a-platelet/material adhesion became much tighter. In the fully activated platelet stage, the platelet becomes thinner and tightly adheres to the substrate. As a result of comparative observation of an adherent platelet on polycarbonate (PC) and on amorphous carbon (a-C:H), it was found that internal granules release was inhibited more remarkably on a-C:H coating rather than on PC. Despite numerous technical difficulties in preparing sectional samples, such a study might prove the essential mechanism of biomaterial-related thrombosis, and it might become possible to modify the surfaces of materials to minimize material-related thrombosis.

Original languageEnglish
Pages (from-to)342-349
Number of pages8
JournalMicroscopy Research and Technique
Volume76
Issue number4
DOIs
Publication statusPublished - 2013 Apr

Fingerprint

Platelet Activation
Platelets
platelets
Transmission Electron Microscopy
Blood Platelets
Chemical activation
activation
Transmission electron microscopy
transmission electron microscopy
polycarbonate
Observation
thrombosis
adhesion
Adhesion
Thrombosis
polycarbonates
Polycarbonates
Pseudopodia
Biocompatible Materials
Blood Coagulation

Keywords

  • Cell-material interface
  • Diamond-like carbon
  • Platelet
  • Transmission electron microscopy

ASJC Scopus subject areas

  • Anatomy
  • Instrumentation
  • Histology
  • Medical Laboratory Technology

Cite this

Ultrastructural characterization of surface-induced platelet activation on artificial materials by transmission electron microscopy. / Yoshimoto, Yukihiro; Hasebe, Terumitsu; Takahashi, Kei; Amari, Masao; Nagashima, So; Kamijo, Aki; Hotta, Atsushi; Takahashi, Koki; Suzuki, Tetsuya.

In: Microscopy Research and Technique, Vol. 76, No. 4, 04.2013, p. 342-349.

Research output: Contribution to journalArticle

Yoshimoto, Yukihiro ; Hasebe, Terumitsu ; Takahashi, Kei ; Amari, Masao ; Nagashima, So ; Kamijo, Aki ; Hotta, Atsushi ; Takahashi, Koki ; Suzuki, Tetsuya. / Ultrastructural characterization of surface-induced platelet activation on artificial materials by transmission electron microscopy. In: Microscopy Research and Technique. 2013 ; Vol. 76, No. 4. pp. 342-349.
@article{69c67abbbbaa40c7a45b630ca108a32c,
title = "Ultrastructural characterization of surface-induced platelet activation on artificial materials by transmission electron microscopy",
abstract = "Platelet adhesion is one of the most pivotal events of blood clotting for artificial surfaces. However, the mechanisms of surface-induced platelet activation have not been fully been elucidated or visualized so far. In this study, we attempted to observe the internal structures and adhesion interfaces of human platelets attached to artificial surfaces by transmission electron microscopy (TEM) during the platelet activation process. We prepared observation samples by a conventional embedding method using EPON 812 resin. The sectioning was sliced perpendicular to the a-platelet/material interface. Observation by TEM indicates that internal granules coalesce in the center of the platelet accompanied by pseudopodial growth in the early stage of platelet activation. Pseudopodia from a platelet attach to the material interface not along a plane but at a point. In addition, along with the process of platelet activation, the gap between the platelet membrane and the material surface at the interface disappeared and a-platelet/material adhesion became much tighter. In the fully activated platelet stage, the platelet becomes thinner and tightly adheres to the substrate. As a result of comparative observation of an adherent platelet on polycarbonate (PC) and on amorphous carbon (a-C:H), it was found that internal granules release was inhibited more remarkably on a-C:H coating rather than on PC. Despite numerous technical difficulties in preparing sectional samples, such a study might prove the essential mechanism of biomaterial-related thrombosis, and it might become possible to modify the surfaces of materials to minimize material-related thrombosis.",
keywords = "Cell-material interface, Diamond-like carbon, Platelet, Transmission electron microscopy",
author = "Yukihiro Yoshimoto and Terumitsu Hasebe and Kei Takahashi and Masao Amari and So Nagashima and Aki Kamijo and Atsushi Hotta and Koki Takahashi and Tetsuya Suzuki",
year = "2013",
month = "4",
doi = "10.1002/jemt.22172",
language = "English",
volume = "76",
pages = "342--349",
journal = "Microscopy Research and Technique",
issn = "1059-910X",
publisher = "Wiley-Liss Inc.",
number = "4",

}

TY - JOUR

T1 - Ultrastructural characterization of surface-induced platelet activation on artificial materials by transmission electron microscopy

AU - Yoshimoto, Yukihiro

AU - Hasebe, Terumitsu

AU - Takahashi, Kei

AU - Amari, Masao

AU - Nagashima, So

AU - Kamijo, Aki

AU - Hotta, Atsushi

AU - Takahashi, Koki

AU - Suzuki, Tetsuya

PY - 2013/4

Y1 - 2013/4

N2 - Platelet adhesion is one of the most pivotal events of blood clotting for artificial surfaces. However, the mechanisms of surface-induced platelet activation have not been fully been elucidated or visualized so far. In this study, we attempted to observe the internal structures and adhesion interfaces of human platelets attached to artificial surfaces by transmission electron microscopy (TEM) during the platelet activation process. We prepared observation samples by a conventional embedding method using EPON 812 resin. The sectioning was sliced perpendicular to the a-platelet/material interface. Observation by TEM indicates that internal granules coalesce in the center of the platelet accompanied by pseudopodial growth in the early stage of platelet activation. Pseudopodia from a platelet attach to the material interface not along a plane but at a point. In addition, along with the process of platelet activation, the gap between the platelet membrane and the material surface at the interface disappeared and a-platelet/material adhesion became much tighter. In the fully activated platelet stage, the platelet becomes thinner and tightly adheres to the substrate. As a result of comparative observation of an adherent platelet on polycarbonate (PC) and on amorphous carbon (a-C:H), it was found that internal granules release was inhibited more remarkably on a-C:H coating rather than on PC. Despite numerous technical difficulties in preparing sectional samples, such a study might prove the essential mechanism of biomaterial-related thrombosis, and it might become possible to modify the surfaces of materials to minimize material-related thrombosis.

AB - Platelet adhesion is one of the most pivotal events of blood clotting for artificial surfaces. However, the mechanisms of surface-induced platelet activation have not been fully been elucidated or visualized so far. In this study, we attempted to observe the internal structures and adhesion interfaces of human platelets attached to artificial surfaces by transmission electron microscopy (TEM) during the platelet activation process. We prepared observation samples by a conventional embedding method using EPON 812 resin. The sectioning was sliced perpendicular to the a-platelet/material interface. Observation by TEM indicates that internal granules coalesce in the center of the platelet accompanied by pseudopodial growth in the early stage of platelet activation. Pseudopodia from a platelet attach to the material interface not along a plane but at a point. In addition, along with the process of platelet activation, the gap between the platelet membrane and the material surface at the interface disappeared and a-platelet/material adhesion became much tighter. In the fully activated platelet stage, the platelet becomes thinner and tightly adheres to the substrate. As a result of comparative observation of an adherent platelet on polycarbonate (PC) and on amorphous carbon (a-C:H), it was found that internal granules release was inhibited more remarkably on a-C:H coating rather than on PC. Despite numerous technical difficulties in preparing sectional samples, such a study might prove the essential mechanism of biomaterial-related thrombosis, and it might become possible to modify the surfaces of materials to minimize material-related thrombosis.

KW - Cell-material interface

KW - Diamond-like carbon

KW - Platelet

KW - Transmission electron microscopy

UR - http://www.scopus.com/inward/record.url?scp=84875591107&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875591107&partnerID=8YFLogxK

U2 - 10.1002/jemt.22172

DO - 10.1002/jemt.22172

M3 - Article

VL - 76

SP - 342

EP - 349

JO - Microscopy Research and Technique

JF - Microscopy Research and Technique

SN - 1059-910X

IS - 4

ER -