Umbilical venous guanosine 3',5'-cyclic phosphate (cGMP) concentration increases in asphyxiated newborns

H. Itoh, N. Sagawa, M. Hasegawa, T. Mori, S. Suga, M. Mukoyama, T. Yoshimasa, Hiroshi Itoh, K. Nakao

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Guanosine 3',5'-cyclic phosphate (cGMP) is known to be the second messenger of natriuretic peptides and nitric oxide (NO). To investigate the involvement of natriuretic peptides in the of the feto-placental circulation, specific radioimmunoassays were used to measure the concentrations of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP) and cGMP in the umbilical venous plasma of normal and asphyxiated newborns. The plasma concentrations of ANP, BNP and cGMP in asphyxiated newborns were 48.3 ± 12.9 pM, 24.5 ± 9.4 pM and 4.4 ± 1.6 nM (mean ± s.e.m., n = 10), respectively. These values were significantly higher than those in the normal newborns (17.4 ± 1.9 pM, 4.7 ± 1.0 pM, and 0.78 ± 0.14 nM, respectively). Moreover, the expression of both ANP-A and ANP-B receptor, biologically active receptors for natriuretic peptides, was detected in term human placenta by Northern blot analysis. The expression of natriuretic peptide receptors was further confirmed by binding assay using [125I]-labelled ANP and solubilized crude membrane preparations of placental tissue. These findings suggest that cGMP is produced in the placenta, at least partly, by the action of ANP and BNP secreted from fetal heart, in pathophysiological conditions such as fetal hypoxia.

Original languageEnglish
Pages (from-to)1515-1519
Number of pages5
JournalReproduction, Fertility and Development
Volume7
Issue number6
DOIs
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

umbilicus
Umbilicus
atrial natriuretic peptide
guanosine
Guanosine
natriuretic peptides
Atrial Natriuretic Factor
Natriuretic Peptides
neonates
Phosphates
phosphates
Brain Natriuretic Peptide
Placenta
placenta
brain
Atrial Natriuretic Factor Receptors
Fetal Hypoxia
Placental Circulation
Fetal Heart
Peptide Receptors

Keywords

  • ANP-A receptor
  • ANP-B receptor
  • atrial natriuretic peptide (ANP)
  • brain natriuretic peptide (BNP)
  • fetal asphyxia
  • placenta

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Reproductive Medicine
  • Developmental Biology
  • Endocrinology

Cite this

Umbilical venous guanosine 3',5'-cyclic phosphate (cGMP) concentration increases in asphyxiated newborns. / Itoh, H.; Sagawa, N.; Hasegawa, M.; Mori, T.; Suga, S.; Mukoyama, M.; Yoshimasa, T.; Itoh, Hiroshi; Nakao, K.

In: Reproduction, Fertility and Development, Vol. 7, No. 6, 1995, p. 1515-1519.

Research output: Contribution to journalArticle

Itoh, H, Sagawa, N, Hasegawa, M, Mori, T, Suga, S, Mukoyama, M, Yoshimasa, T, Itoh, H & Nakao, K 1995, 'Umbilical venous guanosine 3',5'-cyclic phosphate (cGMP) concentration increases in asphyxiated newborns', Reproduction, Fertility and Development, vol. 7, no. 6, pp. 1515-1519. https://doi.org/10.1071/RD9951515
Itoh, H. ; Sagawa, N. ; Hasegawa, M. ; Mori, T. ; Suga, S. ; Mukoyama, M. ; Yoshimasa, T. ; Itoh, Hiroshi ; Nakao, K. / Umbilical venous guanosine 3',5'-cyclic phosphate (cGMP) concentration increases in asphyxiated newborns. In: Reproduction, Fertility and Development. 1995 ; Vol. 7, No. 6. pp. 1515-1519.
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AU - Suga, S.

AU - Mukoyama, M.

AU - Yoshimasa, T.

AU - Itoh, Hiroshi

AU - Nakao, K.

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