Uncoating of influenza virus in endosomes

Akihiko Yoshimura, S. I. Ohnishi

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

The intracellular uncoating site of influenza virus was studied by measuring the fluorescence intensity of probes conjugated to the virus or the isolated hemagglutinin and also by assaying virus replication under various incubation conditions. Acidification of the viral environment was monitored by the decrease in the fluorescence intensity of fluorescein isothiocynate, and transport of the virus particles into secondary lysosomes was assayed by the increase in the fluorescence intensity of fluorescein isothiocyanate diphosphate. The intracellular pH was estimated by the ratio of fluorescence intensities excited at two different wavelengths. It was found that the viral environment became acidified to a pH value of 5.1 to 5.2 within 10 min at 37°C or 1 h at 20°C after endocytosis. Addition of ammonium chloride to the medium rapidly raised the pH to 6.7. Transport of the virus particles into the secondary lysosomes was slower and negligibly low during those incubation periods. Virus replication occurred when the cells were incubated for 10 min at 37°C or for 1 h at 20°C, follwed by incubation in the presence of ammonium chloride for a total of 12 h. These results indicate the uncoating of influenza virus in endosomes before reaching the secondary lysosomes.

Original languageEnglish
Pages (from-to)497-504
Number of pages8
JournalJournal of Virology
Volume51
Issue number2
Publication statusPublished - 1984
Externally publishedYes

Fingerprint

endosomes
Endosomes
Orthomyxoviridae
lysosomes
Lysosomes
Fluorescence
fluorescence
Ammonium Chloride
ammonium chloride
fluorescein
Virus Replication
virus replication
Fluorescein
virion
Virion
Diphosphates
isothiocyanates
Hemagglutinins
endocytosis
hemagglutinins

ASJC Scopus subject areas

  • Immunology

Cite this

Uncoating of influenza virus in endosomes. / Yoshimura, Akihiko; Ohnishi, S. I.

In: Journal of Virology, Vol. 51, No. 2, 1984, p. 497-504.

Research output: Contribution to journalArticle

Yoshimura, A & Ohnishi, SI 1984, 'Uncoating of influenza virus in endosomes', Journal of Virology, vol. 51, no. 2, pp. 497-504.
Yoshimura, Akihiko ; Ohnishi, S. I. / Uncoating of influenza virus in endosomes. In: Journal of Virology. 1984 ; Vol. 51, No. 2. pp. 497-504.
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