Undesigned selection for replication termination of bacterial chromosomes

Nobuaki Kono, Kazuharu Arakawa, Mitsuru Sato, Hirofumi Yoshikawa, Masaru Tomita, Mitsuhiro Itaya

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The oriC DNA replication origin in bacterial chromosomes, the location of which appears to be physically identified, is genetically regulated by relevant molecular machinery. In contrast, the location of the terminus remains obscure for many bacterial replicons, except for terC, the proposed and well-studied chromosome termination site in certain bacteria. The terC locus, which is composed of specific sequences for its binding protein, is located at a site opposite from oriC, exhibiting a symmetric structure around the oriC-terC axis. Here, we investigated Bacillus subtilis 168 strains whose axes were hindered and found that the native terC function was robust. However, eradication of terminus region specific binding protein resulted in the natural terC sites not being used for termination; instead, new termini were selected at a location exactly opposite to oriC. We concluded that replication generally terminates at the loci where the two approaching replisomes meet. This site was automatically selected, and two replisomes moving at the same rate supported symmetrical chromosome structures relative to oriC. The rule, which was even validated by artificial chromosomes irrespective of oriC, should be general for replicons administered by two replisomes.

Original languageEnglish
Pages (from-to)2918-2927
Number of pages10
JournalJournal of Molecular Biology
Volume426
Issue number16
DOIs
Publication statusPublished - 2014 Aug 12

Fingerprint

Bacterial Chromosomes
Replicon
Carrier Proteins
Artificial Chromosomes
Chromosome Structures
Replication Origin
Bacillus subtilis
DNA Replication
Chromosomes
Bacteria

Keywords

  • Bacillus subtilis
  • circular chromosome
  • genomic structure
  • high-throughput sequencing
  • replication terminus

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Undesigned selection for replication termination of bacterial chromosomes. / Kono, Nobuaki; Arakawa, Kazuharu; Sato, Mitsuru; Yoshikawa, Hirofumi; Tomita, Masaru; Itaya, Mitsuhiro.

In: Journal of Molecular Biology, Vol. 426, No. 16, 12.08.2014, p. 2918-2927.

Research output: Contribution to journalArticle

Kono, Nobuaki ; Arakawa, Kazuharu ; Sato, Mitsuru ; Yoshikawa, Hirofumi ; Tomita, Masaru ; Itaya, Mitsuhiro. / Undesigned selection for replication termination of bacterial chromosomes. In: Journal of Molecular Biology. 2014 ; Vol. 426, No. 16. pp. 2918-2927.
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