Unexpectedly Efficient Homing Capacity of Purified Murine Hematopoietic Stem Cells

Yumi Matsuzaki, Kentaro Kinjo, Richard C. Mulligan, Hideyuki Okano

Research output: Contribution to journalArticlepeer-review

249 Citations (Scopus)

Abstract

Single-cell transplantation analysis revealed that the cells that had the strongest dye efflux activity ("Tip"-SP cells) and had the phenotype CD34- c-Kit+ Sca-1+ Lin- (CD34- KSL cells) exhibited very strong proliferation and multilineage differentiation capacity. Ninety-six percent of the lethally irradiated mice that received a single "Tip"-SP CD34- KSL cell showed significant donor cell engraftment for long term. These findings support the hypothesis that "Tip"-SP CD34- KSL cells represent the most primitive hematopoietic stem cells that are capable of migrating into the primary site and surviving and/or proliferating with nearly absolute efficiency. This led us to propose high marrow-seeding efficiency as a specific characteristic of primitive HSCs, in addition to their self-renewal and multipotent capacity.

Original languageEnglish
Pages (from-to)87-93
Number of pages7
JournalImmunity
Volume20
Issue number1
DOIs
Publication statusPublished - 2004 Jan

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

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