Abstract
University of Wisconsin and modified Euro-Collins solutions for pulmonary preservation were compared in a rat orthotopic left lung isotransplant model. Heart-lung blocks of donor rats were flushed with and preserved in one of the preservation solutions at 0 °C. After 6 or 12 hours of cold ischemia, the left lungs were transplanted into recipient rats and repcrfused for 1 hour. Pulmonary function was assessed by measuring oxygen and carbon dioxide tensions in arterial blood after removal of the right lung. Lipid peroxide concentrations were measured as thiobarbiturate acid-reactive substances. The ratios of wet to dry weight of grafts after ischemia and after reperfusion were calculated. Histologic changes of ischemia-reperfusion injury of the lung tissue were evaluated using a graded scale. Oxygen tension after 6 hours of preservation followed by reperfusion was significantly higher with University of Wisconsin solution (308.8 ± 81.1 mm Hg) than with Euro-Collins solution (50.8 ± 17.8 mm Hg; p < 0.001). Carbon dioxide tension in the University of Wisconsin solution group was also significantly lower than in the Euro-Collins solution group (28.2 ± 2.3 versus 46.0 ± 4.5 mm Hg; p < 0.05). Lipid peroxide concentration after 6 hours' preservation in University of Wisconsin solution was significantly lower (0.88 ± 0.07 μmol/g) than that in Euro-Collins solution (1.26 ± 0.12 μmol/g; p < 0.05). After 12 hours of preservation only lipid peroxide concentration with University of Wisconsin solution was significantly lower (1.30 ± 0.09 μmol/g) than with Euro-Collins solution (1.71 ± 0.15 μmol/g; p < 0.05). There were no differences in the other variables. We conclude that University of Wisconsin solution provides superior lung preservation than does Euro-Collins solution in this transplant model.
| Original language | English |
|---|---|
| Pages (from-to) | 240-246 |
| Number of pages | 7 |
| Journal | The Annals of thoracic surgery |
| Volume | 53 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1992 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
- Surgery
Cite this
University of Wisconsin solution for pulmonary preservation in a rat transplant model. / Aeba, Ryo; Keenan, Robert J.; Hardesty, Robert L.; Yousem, Samuel A.; Hamamoto, Isao; Griffith, Bartley P.
In: The Annals of thoracic surgery, Vol. 53, No. 2, 1992, p. 240-246.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - University of Wisconsin solution for pulmonary preservation in a rat transplant model
AU - Aeba, Ryo
AU - Keenan, Robert J.
AU - Hardesty, Robert L.
AU - Yousem, Samuel A.
AU - Hamamoto, Isao
AU - Griffith, Bartley P.
PY - 1992
Y1 - 1992
N2 - University of Wisconsin and modified Euro-Collins solutions for pulmonary preservation were compared in a rat orthotopic left lung isotransplant model. Heart-lung blocks of donor rats were flushed with and preserved in one of the preservation solutions at 0 °C. After 6 or 12 hours of cold ischemia, the left lungs were transplanted into recipient rats and repcrfused for 1 hour. Pulmonary function was assessed by measuring oxygen and carbon dioxide tensions in arterial blood after removal of the right lung. Lipid peroxide concentrations were measured as thiobarbiturate acid-reactive substances. The ratios of wet to dry weight of grafts after ischemia and after reperfusion were calculated. Histologic changes of ischemia-reperfusion injury of the lung tissue were evaluated using a graded scale. Oxygen tension after 6 hours of preservation followed by reperfusion was significantly higher with University of Wisconsin solution (308.8 ± 81.1 mm Hg) than with Euro-Collins solution (50.8 ± 17.8 mm Hg; p < 0.001). Carbon dioxide tension in the University of Wisconsin solution group was also significantly lower than in the Euro-Collins solution group (28.2 ± 2.3 versus 46.0 ± 4.5 mm Hg; p < 0.05). Lipid peroxide concentration after 6 hours' preservation in University of Wisconsin solution was significantly lower (0.88 ± 0.07 μmol/g) than that in Euro-Collins solution (1.26 ± 0.12 μmol/g; p < 0.05). After 12 hours of preservation only lipid peroxide concentration with University of Wisconsin solution was significantly lower (1.30 ± 0.09 μmol/g) than with Euro-Collins solution (1.71 ± 0.15 μmol/g; p < 0.05). There were no differences in the other variables. We conclude that University of Wisconsin solution provides superior lung preservation than does Euro-Collins solution in this transplant model.
AB - University of Wisconsin and modified Euro-Collins solutions for pulmonary preservation were compared in a rat orthotopic left lung isotransplant model. Heart-lung blocks of donor rats were flushed with and preserved in one of the preservation solutions at 0 °C. After 6 or 12 hours of cold ischemia, the left lungs were transplanted into recipient rats and repcrfused for 1 hour. Pulmonary function was assessed by measuring oxygen and carbon dioxide tensions in arterial blood after removal of the right lung. Lipid peroxide concentrations were measured as thiobarbiturate acid-reactive substances. The ratios of wet to dry weight of grafts after ischemia and after reperfusion were calculated. Histologic changes of ischemia-reperfusion injury of the lung tissue were evaluated using a graded scale. Oxygen tension after 6 hours of preservation followed by reperfusion was significantly higher with University of Wisconsin solution (308.8 ± 81.1 mm Hg) than with Euro-Collins solution (50.8 ± 17.8 mm Hg; p < 0.001). Carbon dioxide tension in the University of Wisconsin solution group was also significantly lower than in the Euro-Collins solution group (28.2 ± 2.3 versus 46.0 ± 4.5 mm Hg; p < 0.05). Lipid peroxide concentration after 6 hours' preservation in University of Wisconsin solution was significantly lower (0.88 ± 0.07 μmol/g) than that in Euro-Collins solution (1.26 ± 0.12 μmol/g; p < 0.05). After 12 hours of preservation only lipid peroxide concentration with University of Wisconsin solution was significantly lower (1.30 ± 0.09 μmol/g) than with Euro-Collins solution (1.71 ± 0.15 μmol/g; p < 0.05). There were no differences in the other variables. We conclude that University of Wisconsin solution provides superior lung preservation than does Euro-Collins solution in this transplant model.
UR - http://www.scopus.com/inward/record.url?scp=0026595203&partnerID=8YFLogxK
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U2 - 10.1016/0003-4975(92)91325-4
DO - 10.1016/0003-4975(92)91325-4
M3 - Article
C2 - 1531007
AN - SCOPUS:0026595203
VL - 53
SP - 240
EP - 246
JO - Annals of Thoracic Surgery
JF - Annals of Thoracic Surgery
SN - 0003-4975
IS - 2
ER -