University of Wisconsin solution for pulmonary preservation in a rat transplant model

Ryo Aeba, Robert J. Keenan, Robert L. Hardesty, Samuel A. Yousem, Isao Hamamoto, Bartley P. Griffith

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

University of Wisconsin and modified Euro-Collins solutions for pulmonary preservation were compared in a rat orthotopic left lung isotransplant model. Heart-lung blocks of donor rats were flushed with and preserved in one of the preservation solutions at 0 °C. After 6 or 12 hours of cold ischemia, the left lungs were transplanted into recipient rats and repcrfused for 1 hour. Pulmonary function was assessed by measuring oxygen and carbon dioxide tensions in arterial blood after removal of the right lung. Lipid peroxide concentrations were measured as thiobarbiturate acid-reactive substances. The ratios of wet to dry weight of grafts after ischemia and after reperfusion were calculated. Histologic changes of ischemia-reperfusion injury of the lung tissue were evaluated using a graded scale. Oxygen tension after 6 hours of preservation followed by reperfusion was significantly higher with University of Wisconsin solution (308.8 ± 81.1 mm Hg) than with Euro-Collins solution (50.8 ± 17.8 mm Hg; p < 0.001). Carbon dioxide tension in the University of Wisconsin solution group was also significantly lower than in the Euro-Collins solution group (28.2 ± 2.3 versus 46.0 ± 4.5 mm Hg; p < 0.05). Lipid peroxide concentration after 6 hours' preservation in University of Wisconsin solution was significantly lower (0.88 ± 0.07 μmol/g) than that in Euro-Collins solution (1.26 ± 0.12 μmol/g; p < 0.05). After 12 hours of preservation only lipid peroxide concentration with University of Wisconsin solution was significantly lower (1.30 ± 0.09 μmol/g) than with Euro-Collins solution (1.71 ± 0.15 μmol/g; p < 0.05). There were no differences in the other variables. We conclude that University of Wisconsin solution provides superior lung preservation than does Euro-Collins solution in this transplant model.

Original languageEnglish
Pages (from-to)240-246
Number of pages7
JournalThe Annals of thoracic surgery
Volume53
Issue number2
DOIs
Publication statusPublished - 1992
Externally publishedYes

Fingerprint

Transplants
Lung
Lipid Peroxides
Carbon Dioxide
Reperfusion
Isografts
Oxygen
Cold Ischemia
Heart Block
Reperfusion Injury
Euro-Collins' solution
Arterial Pressure
Ischemia
Weights and Measures
Acids

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery

Cite this

Aeba, R., Keenan, R. J., Hardesty, R. L., Yousem, S. A., Hamamoto, I., & Griffith, B. P. (1992). University of Wisconsin solution for pulmonary preservation in a rat transplant model. The Annals of thoracic surgery, 53(2), 240-246. https://doi.org/10.1016/0003-4975(92)91325-4

University of Wisconsin solution for pulmonary preservation in a rat transplant model. / Aeba, Ryo; Keenan, Robert J.; Hardesty, Robert L.; Yousem, Samuel A.; Hamamoto, Isao; Griffith, Bartley P.

In: The Annals of thoracic surgery, Vol. 53, No. 2, 1992, p. 240-246.

Research output: Contribution to journalArticle

Aeba, R, Keenan, RJ, Hardesty, RL, Yousem, SA, Hamamoto, I & Griffith, BP 1992, 'University of Wisconsin solution for pulmonary preservation in a rat transplant model', The Annals of thoracic surgery, vol. 53, no. 2, pp. 240-246. https://doi.org/10.1016/0003-4975(92)91325-4
Aeba, Ryo ; Keenan, Robert J. ; Hardesty, Robert L. ; Yousem, Samuel A. ; Hamamoto, Isao ; Griffith, Bartley P. / University of Wisconsin solution for pulmonary preservation in a rat transplant model. In: The Annals of thoracic surgery. 1992 ; Vol. 53, No. 2. pp. 240-246.
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