This paper deals with a technique for time-resolved simultaneous measurement of velocity and temperature in wake region. A measurement technique, combining a high-speed particle image velocimetry (PIV 1000 frame per second) with thermo-couple sensing (7-type, 50,μm), has been developed for acquiring time-resolved two-dimensional velocity and temperature data in thermal-fluid flow, simultaneously. To accomplish the simultaneous measurement, thermocouple time constant was compensated by solving inverse problem with experimental time-resolved velocity data. The technique was applied to a wake region behind a heated circular cylinder in steady and unsteady flow. The evolutions of vortical and thermal structures were obtained in order to examine the mechanism of the turbulent heat transfer in such turbulent flows.