Up-regulation of E-cadherin and β-catenin in human hepatocellular carcinoma cell lines by sodium butyrate and interferon-α

T. Masuda, Hidetsugu Saito, F. Kaneko, K. Atsukawa, M. Morita, H. Inagaki, N. Kumagai, K. Tsuchimoto, H. Ishii

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Human E-cadherin is a homophilic cell adhesion molecule and its expression is well preserved in normal human hepatocytes; a decrease in its expression has been observed in poorly differentiated hepatocellular carcinoma cells. We examined the alteration of E-cadherin and catenin expressions caused by differentiation inducers in human hepatocellular carcinoma cells. Hepatocellular carcinoma cell lines, HCC-T and HCC-M, were cultured with all-trans retinoic acid (atra), dexamethasone (dex), sodium butyrate, and interferon-α. E-cadherin expression was only up-regulated by butyrate and interferon-α (ifn-α) in both cell lines, studied by means of fluorescence immunostaining and flow cytometry. The localization of E-cadherin staining was shown at their cell membrane. According to the increase in E-cadherin expression, β-catenin expression appeared at the cell membrane of both cell lines when treated with butyrate and Ifn-α. Such an appearance was not observed when cells were treated with ATRA and DEX. Western blotting showed that α-α and γ,-catenin expression was not changed, while only the expression of β-catenin increased. β-catenin oncogenic activation as a result of amino acid substitutions or interstitial deletions within or including parts of exon 3, which has been demonstrated recently, was not detected in these cell lines by direct deoxyribonucleic acid sequencing. These results suggest that the expression and interaction between E-cadherin and wild-type β-catenin are potentially modulated by butyrate and Ifn-α, and that these two agents are potent inhibitors of hepatocellular carcinoma cell invasion and metastasis.

Original languageEnglish
Pages (from-to)387-394
Number of pages8
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume36
Issue number6
DOIs
Publication statusPublished - 2000 Jan 1

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Catenins
Butyric Acid
Cadherins
Interferons
Hepatocellular Carcinoma
Up-Regulation
Cell Line
Butyrates
Cell Membrane
Cell Adhesion Molecules
Amino Acid Substitution
Tretinoin
Dexamethasone
Hepatocytes
Exons
Flow Cytometry
Fluorescence
Western Blotting
Staining and Labeling
Neoplasm Metastasis

Keywords

  • Adhesion molecule
  • Hepatocellular carcinoma
  • Invasion
  • Metastasis

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Up-regulation of E-cadherin and β-catenin in human hepatocellular carcinoma cell lines by sodium butyrate and interferon-α. / Masuda, T.; Saito, Hidetsugu; Kaneko, F.; Atsukawa, K.; Morita, M.; Inagaki, H.; Kumagai, N.; Tsuchimoto, K.; Ishii, H.

In: In Vitro Cellular and Developmental Biology - Animal, Vol. 36, No. 6, 01.01.2000, p. 387-394.

Research output: Contribution to journalArticle

Masuda, T. ; Saito, Hidetsugu ; Kaneko, F. ; Atsukawa, K. ; Morita, M. ; Inagaki, H. ; Kumagai, N. ; Tsuchimoto, K. ; Ishii, H. / Up-regulation of E-cadherin and β-catenin in human hepatocellular carcinoma cell lines by sodium butyrate and interferon-α. In: In Vitro Cellular and Developmental Biology - Animal. 2000 ; Vol. 36, No. 6. pp. 387-394.
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