Up-regulation of S100P expression by non-steroidal anti-inflammatory drugs and its role in anti-tumorigenic effects

Takushi Namba, Takashi Homan, Tomoko Nishimura, Shinji Mima, Tatsuya Hoshino, Tohru Mizushima

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Epidemiological studies have revealed that prolonged use of non-steroidal anti-inflammatory drugs (NSAIDs) reduces the risk of cancer. Various mechanisms, including induction of apoptosis and inhibition of the growth and invasion of cancer cells, have been implicated in this anti-tumorigenic activity. In this study we focused on S100P, which is known to be overexpressed in clinically isolated tumors and which functions through both intracellular and extracellular mechanisms. We showed the upregulation of S100P expression in human gastric carcinoma cells treated with various NSAIDs, including celecoxib. The celecoxib-mediated up-regulation of S100P was suppressed by the transfection of cells with small interfering RNA for activating transcription factor 4 (ATF4), a transcription factor involved in the endoplasmic reticulum stress response. Furthermore, deletion of ATF4 binding consensus sequence located in the promoter of the S100P gene resulted in inhibition of celecoxib-mediated transcriptional activation of the gene. These results suggest that celecoxib up-regulates the expression of S100P through an ATF4-mediated endoplasmic reticulum stress response. Celecoxib inhibited the growth and induced apoptosis, and these actions could be either suppressed or stimulated by transfection of cells with S100P overexpression plasmid or small interfering RNA, respectively. Celecoxib also inhibited the invasive activity of the cells. Cromolyn, which inhibits the binding of S100P to its receptor, enhanced the celecoxib-mediated inhibition of cell invasion and growth but did not affect apoptosis. These results suggest that S100P affects apoptosis, cell growth, and invasion through either an intracellular or an extracellular mechanism and that the up-regulation of S100P expression by NSAIDs reduces their anti-tumorigenic activity.

Original languageEnglish
Pages (from-to)4158-4167
Number of pages10
JournalJournal of Biological Chemistry
Volume284
Issue number7
DOIs
Publication statusPublished - 2009 Feb 13
Externally publishedYes

Fingerprint

Celecoxib
Anti-Inflammatory Agents
Up-Regulation
Activating Transcription Factor 4
Pharmaceutical Preparations
Cells
Apoptosis
Endoplasmic Reticulum Stress
Growth
Small Interfering RNA
Transfection
Genes
Cromolyn Sodium
Neoplasms
Consensus Sequence
Cell growth
Transcriptional Activation
Tumors
Epidemiologic Studies
Stomach

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Up-regulation of S100P expression by non-steroidal anti-inflammatory drugs and its role in anti-tumorigenic effects. / Namba, Takushi; Homan, Takashi; Nishimura, Tomoko; Mima, Shinji; Hoshino, Tatsuya; Mizushima, Tohru.

In: Journal of Biological Chemistry, Vol. 284, No. 7, 13.02.2009, p. 4158-4167.

Research output: Contribution to journalArticle

Namba, Takushi ; Homan, Takashi ; Nishimura, Tomoko ; Mima, Shinji ; Hoshino, Tatsuya ; Mizushima, Tohru. / Up-regulation of S100P expression by non-steroidal anti-inflammatory drugs and its role in anti-tumorigenic effects. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 7. pp. 4158-4167.
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