Up-regulation of the Ire I-mediated signaling molecule, Bip, in ischemic rat brain

Daisuke Ito, K. Tanaka, Shigeaki Suzuki, T. Dembo, A. Kosakai, Y. Fukuuchi

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The endoplasmic reticulum (ER) is thought to play important roles in various neurological diseases via multifactorial and complex mechanisms. The Ire1-mediated signal is part of one ER signaling pathways; the signal induces the expression of an ER-resident protein, Bip/GRP78, and is thought to be involved in cell death under ER stress. In this study, we examined time-dependent Bip expression after transient middle cerebral artery occlusion and characterized the Bip-positive cells. Ire1- mediated molecules, Bip, were rapidly up-regulated in the ischemic area after 3.5 h recirculation. Their immunoreactivity continued to increase until 24-48h. Immunofluorescence staining revealed Bip up-regulation in ischemic neurons, which were TUNEL positive. Our studies suggest that the Ire1-mediated signal might be associated with ischemic neuronal damage.

Original languageEnglish
Pages (from-to)4023-4028
Number of pages6
JournalNeuroReport
Volume12
Issue number18
Publication statusPublished - 2001 Dec 21

Fingerprint

Endoplasmic Reticulum
Up-Regulation
Brain
Endoplasmic Reticulum Stress
Middle Cerebral Artery Infarction
In Situ Nick-End Labeling
Fluorescent Antibody Technique
Cell Death
Staining and Labeling
Neurons
Proteins

Keywords

  • Cerebral ischemia
  • Endoplasmic reticulum
  • Rat
  • Stroke

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Ito, D., Tanaka, K., Suzuki, S., Dembo, T., Kosakai, A., & Fukuuchi, Y. (2001). Up-regulation of the Ire I-mediated signaling molecule, Bip, in ischemic rat brain. NeuroReport, 12(18), 4023-4028.

Up-regulation of the Ire I-mediated signaling molecule, Bip, in ischemic rat brain. / Ito, Daisuke; Tanaka, K.; Suzuki, Shigeaki; Dembo, T.; Kosakai, A.; Fukuuchi, Y.

In: NeuroReport, Vol. 12, No. 18, 21.12.2001, p. 4023-4028.

Research output: Contribution to journalArticle

Ito, D, Tanaka, K, Suzuki, S, Dembo, T, Kosakai, A & Fukuuchi, Y 2001, 'Up-regulation of the Ire I-mediated signaling molecule, Bip, in ischemic rat brain', NeuroReport, vol. 12, no. 18, pp. 4023-4028.
Ito D, Tanaka K, Suzuki S, Dembo T, Kosakai A, Fukuuchi Y. Up-regulation of the Ire I-mediated signaling molecule, Bip, in ischemic rat brain. NeuroReport. 2001 Dec 21;12(18):4023-4028.
Ito, Daisuke ; Tanaka, K. ; Suzuki, Shigeaki ; Dembo, T. ; Kosakai, A. ; Fukuuchi, Y. / Up-regulation of the Ire I-mediated signaling molecule, Bip, in ischemic rat brain. In: NeuroReport. 2001 ; Vol. 12, No. 18. pp. 4023-4028.
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