Upregulation of metallothionein-I mRNA expression in a rodent model for amyotrophic lateral sclerosis

Shin Ichi Ono, Yuko Endo, Ei Ichi Tokuda, Kumiko Ishige, Kei Ichi Tabata, Satoru Asami, Yoshihisa Ito, Takashi Suzuki

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Metallothionein (MT) mRNA expression was investigated in a rodent model (G93A SOD1 transgenic mouse) for a lethal motor neuron disease, amyotrophic lateral sclerosis (ALS). In 8-wk-old mice that did not yet exhibit motor paralysis, MT-I mRNA expression was already significantly upregulated in the region of the spinal cord responsible for motor paralysis. The expression of another isoform, MT-III, was not changed. In the cerebellum, which is not responsible for motor paralysis in ALS, neither the expression profiles of MT-I nor MT-III were altered. In 16-wk-old mice exhibiting motor paralysis, the expression of MT-I mRNA remained upregulated and the MT-III level tended to be elevated. Although no significant differences were found in the levels of both isoforms in the liver or kidney of 8-wk-old mice, the MT-I mRNA expression level was significantly upregulated in the kidney and liver of 16-wk-old mice. These results indicated that the MT-I isoform, but not the MT-III isoform, is associated with motor neuron death in ALS and suggested that the disease might be a systemic disorder to which the spinal cord is particularly susceptible.

Original languageEnglish
Pages (from-to)93-104
Number of pages12
JournalBiological Trace Element Research
Volume113
Issue number1
DOIs
Publication statusPublished - 2006 Oct
Externally publishedYes

Fingerprint

Metallothionein
Amyotrophic Lateral Sclerosis
Rodentia
Up-Regulation
Paralysis
Messenger RNA
Protein Isoforms
Liver
Neurons
Spinal Cord
Kidney
Motor Neurons
Cerebellum
Transgenic Mice
growth inhibitory factor

Keywords

  • Amyotrophic lateral sclerosis
  • Kidney
  • Liver
  • Metallothionein-I
  • Metallothionein-III
  • Rodent model
  • Spinal cord

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Ono, S. I., Endo, Y., Tokuda, E. I., Ishige, K., Tabata, K. I., Asami, S., ... Suzuki, T. (2006). Upregulation of metallothionein-I mRNA expression in a rodent model for amyotrophic lateral sclerosis. Biological Trace Element Research, 113(1), 93-104. https://doi.org/10.1385/BTER:113:1:93

Upregulation of metallothionein-I mRNA expression in a rodent model for amyotrophic lateral sclerosis. / Ono, Shin Ichi; Endo, Yuko; Tokuda, Ei Ichi; Ishige, Kumiko; Tabata, Kei Ichi; Asami, Satoru; Ito, Yoshihisa; Suzuki, Takashi.

In: Biological Trace Element Research, Vol. 113, No. 1, 10.2006, p. 93-104.

Research output: Contribution to journalArticle

Ono, SI, Endo, Y, Tokuda, EI, Ishige, K, Tabata, KI, Asami, S, Ito, Y & Suzuki, T 2006, 'Upregulation of metallothionein-I mRNA expression in a rodent model for amyotrophic lateral sclerosis', Biological Trace Element Research, vol. 113, no. 1, pp. 93-104. https://doi.org/10.1385/BTER:113:1:93
Ono, Shin Ichi ; Endo, Yuko ; Tokuda, Ei Ichi ; Ishige, Kumiko ; Tabata, Kei Ichi ; Asami, Satoru ; Ito, Yoshihisa ; Suzuki, Takashi. / Upregulation of metallothionein-I mRNA expression in a rodent model for amyotrophic lateral sclerosis. In: Biological Trace Element Research. 2006 ; Vol. 113, No. 1. pp. 93-104.
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