Use of isolated mature osteoblasts in abundance acts as desired-shaped bone regeneration in combination with a modified poly-DL-lactic-co-glycolic acid (PLGA)-collagen sponge

Kensuke Ochi, Gouping Chen, Takashi Ushida, Satoshi Gojo, Kaoru Segawa, Hitoshi Tai, Kenju Ueno, Hiroyuki Ohkawa, Taisuke Mori, Akira Yamaguchi, Yoshiaki Toyama, Jun Ichi Hata, Akihiro Umezawa

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Control led regeneration of bone or cartilage has recently begun to facilitate a host of novel clinical treatments. An osteoblast line, which we isolated is able to form new bone matrix in vivo within 2 days and exhibits a mature osteoblast phenotype both in vitro and in vivo. Using these cells, we show that cuboidal bones can be generated into a predesigned shaped-bone with high-density bone trabeculae when used in combination with a modified poly -DL-lactic-co-glycolic acid (PLGA)-collagen sponge. PLGA coated with collagen gel serves as a good scaffold for osteoblasts. These results indicate that mature osteoblasts, in combination with a scaffold such as PLGA-collagen sponge, show promise for use in a custom-shaped bone regeneration tool for both basic research into osteogenesis and for development of therapeutic applications.

Original languageEnglish
Pages (from-to)45-53
Number of pages9
JournalJournal of Cellular Physiology
Volume194
Issue number1
DOIs
Publication statusPublished - 2003 Jan 1

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glycolic acid
Bone Regeneration
Osteoblasts
Porifera
Bone
Collagen
Bone and Bones
Scaffolds
Bone Matrix
Osteogenesis
Bone Density
Cartilage
Gels
Phenotype
polylactic acid-polyglycolic acid copolymer
Milk
Research

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Use of isolated mature osteoblasts in abundance acts as desired-shaped bone regeneration in combination with a modified poly-DL-lactic-co-glycolic acid (PLGA)-collagen sponge. / Ochi, Kensuke; Chen, Gouping; Ushida, Takashi; Gojo, Satoshi; Segawa, Kaoru; Tai, Hitoshi; Ueno, Kenju; Ohkawa, Hiroyuki; Mori, Taisuke; Yamaguchi, Akira; Toyama, Yoshiaki; Hata, Jun Ichi; Umezawa, Akihiro.

In: Journal of Cellular Physiology, Vol. 194, No. 1, 01.01.2003, p. 45-53.

Research output: Contribution to journalArticle

Ochi, K, Chen, G, Ushida, T, Gojo, S, Segawa, K, Tai, H, Ueno, K, Ohkawa, H, Mori, T, Yamaguchi, A, Toyama, Y, Hata, JI & Umezawa, A 2003, 'Use of isolated mature osteoblasts in abundance acts as desired-shaped bone regeneration in combination with a modified poly-DL-lactic-co-glycolic acid (PLGA)-collagen sponge', Journal of Cellular Physiology, vol. 194, no. 1, pp. 45-53. https://doi.org/10.1002/jcp.10185
Ochi, Kensuke ; Chen, Gouping ; Ushida, Takashi ; Gojo, Satoshi ; Segawa, Kaoru ; Tai, Hitoshi ; Ueno, Kenju ; Ohkawa, Hiroyuki ; Mori, Taisuke ; Yamaguchi, Akira ; Toyama, Yoshiaki ; Hata, Jun Ichi ; Umezawa, Akihiro. / Use of isolated mature osteoblasts in abundance acts as desired-shaped bone regeneration in combination with a modified poly-DL-lactic-co-glycolic acid (PLGA)-collagen sponge. In: Journal of Cellular Physiology. 2003 ; Vol. 194, No. 1. pp. 45-53.
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