Use of perfluorocarboxylic acids to trick cytochrome P450BM3 into initiating the hydroxylation of gaseous alkanes

Norifumi Kawakami; Osami Shoji; Yoshihito Watanabe

doi:10.1002/anie.201007975

Use of perfluorocarboxylic acids to trick cytochrome P450BM3 into initiating the hydroxylation of gaseous alkanes

Norifumi Kawakami, Osami Shoji, Yoshihito Watanabe

Research output: Contribution to journal › Article › peer-review

104 Citations (Scopus)

Abstract

It has long been believed that the fatty acid hydroxylase wild-type P450BM3 is unable to oxidize gaseous alkanes. However, the simple addition of a perfluorocarboxylic acid as a dummy substrate to initiate the P450BM3 catalytic cycle enabled the efficient hydroxylation of butane and propane (see picture).

Original language	English
Pages (from-to)	5315-5318
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	50
Issue number	23
DOIs	https://doi.org/10.1002/anie.201007975
Publication status	Published - 2011 May 27
Externally published	Yes

Keywords

cytochromes
enzyme catalysis
gaseous alkanes
hydroxylation
perfluorocarboxylic acids

ASJC Scopus subject areas

Catalysis
Chemistry(all)

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abstract = "It has long been believed that the fatty acid hydroxylase wild-type P450BM3 is unable to oxidize gaseous alkanes. However, the simple addition of a perfluorocarboxylic acid as a dummy substrate to initiate the P450BM3 catalytic cycle enabled the efficient hydroxylation of butane and propane (see picture).",

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AU - Shoji, Osami

AU - Watanabe, Yoshihito

PY - 2011/5/27

Y1 - 2011/5/27

N2 - It has long been believed that the fatty acid hydroxylase wild-type P450BM3 is unable to oxidize gaseous alkanes. However, the simple addition of a perfluorocarboxylic acid as a dummy substrate to initiate the P450BM3 catalytic cycle enabled the efficient hydroxylation of butane and propane (see picture).

AB - It has long been believed that the fatty acid hydroxylase wild-type P450BM3 is unable to oxidize gaseous alkanes. However, the simple addition of a perfluorocarboxylic acid as a dummy substrate to initiate the P450BM3 catalytic cycle enabled the efficient hydroxylation of butane and propane (see picture).

KW - cytochromes

KW - enzyme catalysis

KW - gaseous alkanes

KW - hydroxylation

KW - perfluorocarboxylic acids

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Use of perfluorocarboxylic acids to trick cytochrome P450BM3 into initiating the hydroxylation of gaseous alkanes

Abstract

Keywords

ASJC Scopus subject areas

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