Use of perfluorocarboxylic acids to trick cytochrome P450BM3 into initiating the hydroxylation of gaseous alkanes

Norifumi Kawakami, Osami Shoji, Yoshihito Watanabe

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

It has long been believed that the fatty acid hydroxylase wild-type P450BM3 is unable to oxidize gaseous alkanes. However, the simple addition of a perfluorocarboxylic acid as a dummy substrate to initiate the P450BM3 catalytic cycle enabled the efficient hydroxylation of butane and propane (see picture).

Original languageEnglish
Pages (from-to)5315-5318
Number of pages4
JournalAngewandte Chemie - International Edition
Volume50
Issue number23
DOIs
Publication statusPublished - 2011 May 27
Externally publishedYes

Fingerprint

Propane
Hydroxylation
Alkanes
Butane
Cytochromes
Mixed Function Oxygenases
Fatty acids
Paraffins
Fatty Acids
Proteins
Acids
Substrates
butane

Keywords

  • cytochromes
  • enzyme catalysis
  • gaseous alkanes
  • hydroxylation
  • perfluorocarboxylic acids

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis

Cite this

Use of perfluorocarboxylic acids to trick cytochrome P450BM3 into initiating the hydroxylation of gaseous alkanes. / Kawakami, Norifumi; Shoji, Osami; Watanabe, Yoshihito.

In: Angewandte Chemie - International Edition, Vol. 50, No. 23, 27.05.2011, p. 5315-5318.

Research output: Contribution to journalArticle

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