Usefulness of the DNA-HLA class II typing in corneal transplantation

J. Shimazaki, Kazuo Tsubota, F. Tsuchida, T. Sato, M. Hagihara, K. Tsuji

Research output: Contribution to journalArticle

Abstract

DNA-HLA (human leukocyte antigen) typing was performed for ocular tissues in order to determine the usefulness of the method in corneal transplantation. Each type of ocular tissue was dissected from eye bank eyes (n=3). DNA was extracted, and HLA-DRB1, DQB1, and DPB1 genes were amplified using PCR (polymerase chain reaction) method. DNA can he extracted anti amplified from each ocular tissue except for the crystalline lens, hut DNA from iris and choroid can he amplified only after diluting the samples 10 to 100 times. HLA class II antigens were successfully determined in these ocular tissues by the RFLP (restriction fragment length polymorphism) method. The method was applied to the corneal tissues of donor and recipient used for the corneal transplantation (n=7). HLA class II antigens can be determined in both the donor and recipient corneal samples, and the results of recipients' HLA typing were the same as those determined using the blood samples. These results indicate that the DNA-HLA typing can be used for ocular tissues. Since DNA-HLA typing is more accurate than the conventional serological typing, the method is promising for the study of HLA class II typing in corneal transplantation.

Original languageEnglish
Pages (from-to)1248-1253
Number of pages6
JournalJournal of Japanese Ophthalmological Society
Volume99
Issue number11
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Corneal Transplantation
Histocompatibility Testing
DNA
HLA Antigens
DNA Fingerprinting
Histocompatibility Antigens Class II
Eye Banks
HLA-DRB1 Chains
Crystalline Lens
Choroid
Iris
Restriction Fragment Length Polymorphisms
Tissue Donors
Polymerase Chain Reaction
Genes

Keywords

  • Corneal transplantation
  • DNA
  • HLA
  • PCR-RFLP analysis

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Shimazaki, J., Tsubota, K., Tsuchida, F., Sato, T., Hagihara, M., & Tsuji, K. (1995). Usefulness of the DNA-HLA class II typing in corneal transplantation. Journal of Japanese Ophthalmological Society, 99(11), 1248-1253.

Usefulness of the DNA-HLA class II typing in corneal transplantation. / Shimazaki, J.; Tsubota, Kazuo; Tsuchida, F.; Sato, T.; Hagihara, M.; Tsuji, K.

In: Journal of Japanese Ophthalmological Society, Vol. 99, No. 11, 1995, p. 1248-1253.

Research output: Contribution to journalArticle

Shimazaki, J, Tsubota, K, Tsuchida, F, Sato, T, Hagihara, M & Tsuji, K 1995, 'Usefulness of the DNA-HLA class II typing in corneal transplantation', Journal of Japanese Ophthalmological Society, vol. 99, no. 11, pp. 1248-1253.
Shimazaki, J. ; Tsubota, Kazuo ; Tsuchida, F. ; Sato, T. ; Hagihara, M. ; Tsuji, K. / Usefulness of the DNA-HLA class II typing in corneal transplantation. In: Journal of Japanese Ophthalmological Society. 1995 ; Vol. 99, No. 11. pp. 1248-1253.
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