Using Mahalanobis distance to compare genomic signatures between bacterial plasmids and chromosomes

Haruo Suzuki, Masahiro Sota, Celeste J. Brown, Eva M. Top

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Plasmids are ubiquitous mobile elements that serve as a pool of many host beneficial traits such as antibiotic resistance in bacterial communities. To understand the importance of plasmids in horizontal gene transfer, we need to gain insight into the 'evolutionary history' of these plasmids, i.e. the range of hosts in which they have evolved. Since extensive data support the proposal that foreign DNA acquires the host's nucleotide composition during long-term residence, comparison of nucleotide composition of plasmids and chromosomes could shed light on a plasmid's evolutionary history. The average absolute dinucleotide relative abundance difference, termed δ-distance, has been commonly used to measure differences in dinucleotide composition, or 'genomic signature', between bacterial chromosomes and plasmids. Here, we introduce the Mahalanobis distance, which takes into account the variance-covariance structure of the chromosome signatures. We demonstrate that the Mahalanobis distance is better than the δ-distance at measuring genomic signature differences between plasmids and chromosomes of potential hosts. We illustrate the usefulness of this metric for proposing candidate long-term hosts for plasmids, focusing on the virulence plasmids pXO1 from Bacillus anthracis, and pO157 from Escherichia coli O157:H7, as well as the broad host range multi-drug resistance plasmid pB10 from an unknown host.

Original languageEnglish
JournalNucleic Acids Research
Volume36
Issue number22
DOIs
Publication statusPublished - 2008 Dec
Externally publishedYes

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Bacterial Chromosomes
Plasmids
Host Specificity
Nucleotides
Chromosomes
History
Bacterial Drug Resistance
Chromosome Structures
Bacillus anthracis
Horizontal Gene Transfer
Escherichia coli O157
Multiple Drug Resistance
Virulence

ASJC Scopus subject areas

  • Genetics

Cite this

Using Mahalanobis distance to compare genomic signatures between bacterial plasmids and chromosomes. / Suzuki, Haruo; Sota, Masahiro; Brown, Celeste J.; Top, Eva M.

In: Nucleic Acids Research, Vol. 36, No. 22, 12.2008.

Research output: Contribution to journalArticle

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