Abstract
We recently demonstrated that the zygotic gene Zscan5b is involved in maintaining the genomic integrity of pluripotent stem cells (PSCs) during mitosis and that Zscan5b gene expression can prevent chromosomal abnormalities. Here, we provide a detailed protocol for the transfection of mouse embryonic stem cells (mESCs) and mouse-induced PSCs (miPSCs) with a Zscan5b constitutive expression plasmid, using the piggyBac transposon gene expression vector system. For complete details on the use and execution of this protocol, please refer to Ogawa et al. (2019).
Original language | English |
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Article number | 100811 |
Journal | STAR Protocols |
Volume | 2 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2021 Sep 17 |
Keywords
- Cell Biology
- Cell culture
- Flow Cytometry/Mass Cytometry
- Genomics
- Molecular Biology
- Single Cell
- Stem Cells
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Neuroscience(all)
- Immunology and Microbiology(all)
- Medicine(all)