Using piggyBac transposon gene expression vectors to transfect Zscan5b gene into mouse pluripotent stem cells

Mitsutoshi Yamada, Tohru Sugawara, Shinju Usami, Rina Nakanishi, Hidenori Akustu

Research output: Contribution to journalArticlepeer-review

Abstract

We recently demonstrated that the zygotic gene Zscan5b is involved in maintaining the genomic integrity of pluripotent stem cells (PSCs) during mitosis and that Zscan5b gene expression can prevent chromosomal abnormalities. Here, we provide a detailed protocol for the transfection of mouse embryonic stem cells (mESCs) and mouse-induced PSCs (miPSCs) with a Zscan5b constitutive expression plasmid, using the piggyBac transposon gene expression vector system. For complete details on the use and execution of this protocol, please refer to Ogawa et al. (2019).

Original languageEnglish
Article number100811
JournalSTAR Protocols
Volume2
Issue number3
DOIs
Publication statusPublished - 2021 Sep 17

Keywords

  • Cell Biology
  • Cell culture
  • Flow Cytometry/Mass Cytometry
  • Genomics
  • Molecular Biology
  • Single Cell
  • Stem Cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

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