Abstract
Aim: Uterine transplantation (UTx) is a potential option for child-bearing in women with uterine infertility. Recovery of uterine function after allogeneic UTx in non-human primates has not been reported. The objective of this study is to establish the functional uterine transplant model in non-human primates. Methods: Uteri of two cynomolgus monkeys were simultaneously removed, cooled at 4°C and perfused with heparin saline. The uteri were interchanged with each other and then orthotopically transplanted. Immunosuppressive protocols included use of three agents (tacrolimus, mycophenolate mofetil and methylprednisolone) in case 1 and two agents (tacrolimus and methylprednisolone) in case 2. Transabdominal ultrasonography, vaginoscopy and biopsy of the transplanted uterine cervix were routinely conducted to monitor rejection after surgery. Results: The blood concentration of tacrolimus decreased 11 days after surgery and evidence of rejection was found in biopsy of the uterine cervix in both cases. The suspected rejection disappeared 23 days after surgery in case 1 and temporary menstruation resumed at 3 months after surgery. In case 2, blood flow to the uterine artery gradually decreased and the uterus resulted in atrophy due to ischemia, which has been triggered by rejection. Conclusion: Allogeneic UTx in the cynomolgus monkeys resulted in temporary recovery of menstruation with three immunosuppressants and uterine atrophy with two immunosuppressants. This preliminary experience suggests that recovery of uterine function after allogeneic UTx in non-human primates is possible but more experiments are required.
| Original language | English |
|---|---|
| Pages (from-to) | 907-918 |
| Number of pages | 12 |
| Journal | Journal of Obstetrics and Gynaecology Research |
| Volume | 40 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2014 |
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Keywords
- Cynomolgus macaque
- Immunosuppressants
- Indocyanine green
- Uterine factor infertility
- Uterus transplantation
ASJC Scopus subject areas
- Obstetrics and Gynaecology
- Medicine(all)
Cite this
Uterus allotransplantation in cynomolgus macaque : A preliminary experience with non-human primate models. / Kisu, Iori; Mihara, Makoto; Banno, Kouji; Hara, Hisako; Masugi, Yohei; Araki, Jun; Iida, Takuya; Yamada, Yohei; Kato, Yojiro; Shiina, Takashi; Suganuma, Nobuhiko; Aoki, Daisuke.
In: Journal of Obstetrics and Gynaecology Research, Vol. 40, No. 4, 2014, p. 907-918.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Uterus allotransplantation in cynomolgus macaque
T2 - A preliminary experience with non-human primate models
AU - Kisu, Iori
AU - Mihara, Makoto
AU - Banno, Kouji
AU - Hara, Hisako
AU - Masugi, Yohei
AU - Araki, Jun
AU - Iida, Takuya
AU - Yamada, Yohei
AU - Kato, Yojiro
AU - Shiina, Takashi
AU - Suganuma, Nobuhiko
AU - Aoki, Daisuke
PY - 2014
Y1 - 2014
N2 - Aim: Uterine transplantation (UTx) is a potential option for child-bearing in women with uterine infertility. Recovery of uterine function after allogeneic UTx in non-human primates has not been reported. The objective of this study is to establish the functional uterine transplant model in non-human primates. Methods: Uteri of two cynomolgus monkeys were simultaneously removed, cooled at 4°C and perfused with heparin saline. The uteri were interchanged with each other and then orthotopically transplanted. Immunosuppressive protocols included use of three agents (tacrolimus, mycophenolate mofetil and methylprednisolone) in case 1 and two agents (tacrolimus and methylprednisolone) in case 2. Transabdominal ultrasonography, vaginoscopy and biopsy of the transplanted uterine cervix were routinely conducted to monitor rejection after surgery. Results: The blood concentration of tacrolimus decreased 11 days after surgery and evidence of rejection was found in biopsy of the uterine cervix in both cases. The suspected rejection disappeared 23 days after surgery in case 1 and temporary menstruation resumed at 3 months after surgery. In case 2, blood flow to the uterine artery gradually decreased and the uterus resulted in atrophy due to ischemia, which has been triggered by rejection. Conclusion: Allogeneic UTx in the cynomolgus monkeys resulted in temporary recovery of menstruation with three immunosuppressants and uterine atrophy with two immunosuppressants. This preliminary experience suggests that recovery of uterine function after allogeneic UTx in non-human primates is possible but more experiments are required.
AB - Aim: Uterine transplantation (UTx) is a potential option for child-bearing in women with uterine infertility. Recovery of uterine function after allogeneic UTx in non-human primates has not been reported. The objective of this study is to establish the functional uterine transplant model in non-human primates. Methods: Uteri of two cynomolgus monkeys were simultaneously removed, cooled at 4°C and perfused with heparin saline. The uteri were interchanged with each other and then orthotopically transplanted. Immunosuppressive protocols included use of three agents (tacrolimus, mycophenolate mofetil and methylprednisolone) in case 1 and two agents (tacrolimus and methylprednisolone) in case 2. Transabdominal ultrasonography, vaginoscopy and biopsy of the transplanted uterine cervix were routinely conducted to monitor rejection after surgery. Results: The blood concentration of tacrolimus decreased 11 days after surgery and evidence of rejection was found in biopsy of the uterine cervix in both cases. The suspected rejection disappeared 23 days after surgery in case 1 and temporary menstruation resumed at 3 months after surgery. In case 2, blood flow to the uterine artery gradually decreased and the uterus resulted in atrophy due to ischemia, which has been triggered by rejection. Conclusion: Allogeneic UTx in the cynomolgus monkeys resulted in temporary recovery of menstruation with three immunosuppressants and uterine atrophy with two immunosuppressants. This preliminary experience suggests that recovery of uterine function after allogeneic UTx in non-human primates is possible but more experiments are required.
KW - Cynomolgus macaque
KW - Immunosuppressants
KW - Indocyanine green
KW - Uterine factor infertility
KW - Uterus transplantation
UR - http://www.scopus.com/inward/record.url?scp=84903635137&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84903635137&partnerID=8YFLogxK
U2 - 10.1111/jog.12302
DO - 10.1111/jog.12302
M3 - Article
VL - 40
SP - 907
EP - 918
JO - Asia-Oceania Journal of Obstetrics and Gynaecology
JF - Asia-Oceania Journal of Obstetrics and Gynaecology
SN - 1341-8076
IS - 4
ER -