Utility of 10 immunohistochemical markers including novel markers (desmocollin-3, glypican 3, S100A2, S100A7, and Sox-2) for differential diagnosis of squamous cell carcinoma from adenocarcinoma of the lung

Koji Tsuta, Yuko Tanabe, Akihiko Yoshida, Fumiaki Takahashi, Akiko M. Maeshima, Hisao Asamura, Hitoshi Tsuda

Research output: Contribution to journalArticle

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Abstract

Introduction: Recent clinical trials revealed that accurate histologic typing of non-small cell lung cancer, especially squamous cell carcinoma (SCC), is essential. Patients and Methods: We analyzed 10 antibodies expression in 150 SCC cases (53 well-, 51 moderately, and 46 poorly differentiated cases) and 159 adenocarcinoma (AC) cases (49 well-, 52 moderately, and 58 poorly differentiated cases). Results: In all SCC and AC cases, p63 was the most sensitive marker for SCC (98.7%), followed by high-molecular-weight (HM) cytokeratin (CK) (97.3%), CK5/6 (93.3%), Sox2 (80%), thrombomodulin (79.3%), desmocollin-3 (72.7%), S100A7 (70.7%), S100A2 (63.3%), and glypican-3 (46.7%). Desmocollin-3 was the most specific marker for SCC (100%), followed by CK5/6 (98%), Sox2 (95.5%), glypican-3 (92.4%), S100A7 (86.8%), thrombomodulin (79.9%), S100A2 (64.6%), p63 (51.6%), and HMCK (33.3%). Thyroid transcription factor-1 (TTF-1) expression was observed in 87.4% of AC cases and 2.0% of SCC cases. When analyzing only poorly differentiated tumors, HMCK was the most sensitive marker for SCC (100%), followed by p63 (97.8%), CK5/6 (87.0%), Sox2 (71.7%), thrombomodulin (58.7%), desmocollin-3 (52.2%), S100A2 (50%), glypican-3 (45.7%), and S100A7 (45.7%). Desmocollin-3 was the most specific marker for poorly differentiated SCC (100%), followed by CK5/6 (98.3%), glypican-3 (94.8%), Sox2 (94.8%), S100A2 (81%), S100A7 (75.9%), thrombomodulin (72.4%), p63 (48.3%), and HMCK (36.8%). The 10-fold crossvalidated classification and regression tree analysis revealed that the combination of CK5/6 and TTF-1 was the best immunohistochemical marker panel for the differentiation between SCC and AC. Conclusion: CK5/6 is the best marker for differentiating SCC and AC, irrespective of the histological grade of the tumor. Thus, the combination of CK5/6 and TTF-1 is the most recommended combination of immunohistochemical markers.

Original languageEnglish
Pages (from-to)1190-1199
Number of pages10
JournalJournal of Thoracic Oncology
Volume6
Issue number7
DOIs
Publication statusPublished - 2011 Jul
Externally publishedYes

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Desmocollins
Glypicans
Squamous Cell Carcinoma
Differential Diagnosis
Thrombomodulin
Adenocarcinoma
Adenocarcinoma of lung
Differentiation Antigens
Keratins
Non-Small Cell Lung Carcinoma
Neoplasms

Keywords

  • Cytokeratin 5/6
  • Desmocollin-3
  • Lung
  • Sox2
  • Squamous cell carcinoma

ASJC Scopus subject areas

  • Oncology
  • Pulmonary and Respiratory Medicine

Cite this

Utility of 10 immunohistochemical markers including novel markers (desmocollin-3, glypican 3, S100A2, S100A7, and Sox-2) for differential diagnosis of squamous cell carcinoma from adenocarcinoma of the lung. / Tsuta, Koji; Tanabe, Yuko; Yoshida, Akihiko; Takahashi, Fumiaki; Maeshima, Akiko M.; Asamura, Hisao; Tsuda, Hitoshi.

In: Journal of Thoracic Oncology, Vol. 6, No. 7, 07.2011, p. 1190-1199.

Research output: Contribution to journalArticle

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title = "Utility of 10 immunohistochemical markers including novel markers (desmocollin-3, glypican 3, S100A2, S100A7, and Sox-2) for differential diagnosis of squamous cell carcinoma from adenocarcinoma of the lung",
abstract = "Introduction: Recent clinical trials revealed that accurate histologic typing of non-small cell lung cancer, especially squamous cell carcinoma (SCC), is essential. Patients and Methods: We analyzed 10 antibodies expression in 150 SCC cases (53 well-, 51 moderately, and 46 poorly differentiated cases) and 159 adenocarcinoma (AC) cases (49 well-, 52 moderately, and 58 poorly differentiated cases). Results: In all SCC and AC cases, p63 was the most sensitive marker for SCC (98.7{\%}), followed by high-molecular-weight (HM) cytokeratin (CK) (97.3{\%}), CK5/6 (93.3{\%}), Sox2 (80{\%}), thrombomodulin (79.3{\%}), desmocollin-3 (72.7{\%}), S100A7 (70.7{\%}), S100A2 (63.3{\%}), and glypican-3 (46.7{\%}). Desmocollin-3 was the most specific marker for SCC (100{\%}), followed by CK5/6 (98{\%}), Sox2 (95.5{\%}), glypican-3 (92.4{\%}), S100A7 (86.8{\%}), thrombomodulin (79.9{\%}), S100A2 (64.6{\%}), p63 (51.6{\%}), and HMCK (33.3{\%}). Thyroid transcription factor-1 (TTF-1) expression was observed in 87.4{\%} of AC cases and 2.0{\%} of SCC cases. When analyzing only poorly differentiated tumors, HMCK was the most sensitive marker for SCC (100{\%}), followed by p63 (97.8{\%}), CK5/6 (87.0{\%}), Sox2 (71.7{\%}), thrombomodulin (58.7{\%}), desmocollin-3 (52.2{\%}), S100A2 (50{\%}), glypican-3 (45.7{\%}), and S100A7 (45.7{\%}). Desmocollin-3 was the most specific marker for poorly differentiated SCC (100{\%}), followed by CK5/6 (98.3{\%}), glypican-3 (94.8{\%}), Sox2 (94.8{\%}), S100A2 (81{\%}), S100A7 (75.9{\%}), thrombomodulin (72.4{\%}), p63 (48.3{\%}), and HMCK (36.8{\%}). The 10-fold crossvalidated classification and regression tree analysis revealed that the combination of CK5/6 and TTF-1 was the best immunohistochemical marker panel for the differentiation between SCC and AC. Conclusion: CK5/6 is the best marker for differentiating SCC and AC, irrespective of the histological grade of the tumor. Thus, the combination of CK5/6 and TTF-1 is the most recommended combination of immunohistochemical markers.",
keywords = "Cytokeratin 5/6, Desmocollin-3, Lung, Sox2, Squamous cell carcinoma",
author = "Koji Tsuta and Yuko Tanabe and Akihiko Yoshida and Fumiaki Takahashi and Maeshima, {Akiko M.} and Hisao Asamura and Hitoshi Tsuda",
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T1 - Utility of 10 immunohistochemical markers including novel markers (desmocollin-3, glypican 3, S100A2, S100A7, and Sox-2) for differential diagnosis of squamous cell carcinoma from adenocarcinoma of the lung

AU - Tsuta, Koji

AU - Tanabe, Yuko

AU - Yoshida, Akihiko

AU - Takahashi, Fumiaki

AU - Maeshima, Akiko M.

AU - Asamura, Hisao

AU - Tsuda, Hitoshi

PY - 2011/7

Y1 - 2011/7

N2 - Introduction: Recent clinical trials revealed that accurate histologic typing of non-small cell lung cancer, especially squamous cell carcinoma (SCC), is essential. Patients and Methods: We analyzed 10 antibodies expression in 150 SCC cases (53 well-, 51 moderately, and 46 poorly differentiated cases) and 159 adenocarcinoma (AC) cases (49 well-, 52 moderately, and 58 poorly differentiated cases). Results: In all SCC and AC cases, p63 was the most sensitive marker for SCC (98.7%), followed by high-molecular-weight (HM) cytokeratin (CK) (97.3%), CK5/6 (93.3%), Sox2 (80%), thrombomodulin (79.3%), desmocollin-3 (72.7%), S100A7 (70.7%), S100A2 (63.3%), and glypican-3 (46.7%). Desmocollin-3 was the most specific marker for SCC (100%), followed by CK5/6 (98%), Sox2 (95.5%), glypican-3 (92.4%), S100A7 (86.8%), thrombomodulin (79.9%), S100A2 (64.6%), p63 (51.6%), and HMCK (33.3%). Thyroid transcription factor-1 (TTF-1) expression was observed in 87.4% of AC cases and 2.0% of SCC cases. When analyzing only poorly differentiated tumors, HMCK was the most sensitive marker for SCC (100%), followed by p63 (97.8%), CK5/6 (87.0%), Sox2 (71.7%), thrombomodulin (58.7%), desmocollin-3 (52.2%), S100A2 (50%), glypican-3 (45.7%), and S100A7 (45.7%). Desmocollin-3 was the most specific marker for poorly differentiated SCC (100%), followed by CK5/6 (98.3%), glypican-3 (94.8%), Sox2 (94.8%), S100A2 (81%), S100A7 (75.9%), thrombomodulin (72.4%), p63 (48.3%), and HMCK (36.8%). The 10-fold crossvalidated classification and regression tree analysis revealed that the combination of CK5/6 and TTF-1 was the best immunohistochemical marker panel for the differentiation between SCC and AC. Conclusion: CK5/6 is the best marker for differentiating SCC and AC, irrespective of the histological grade of the tumor. Thus, the combination of CK5/6 and TTF-1 is the most recommended combination of immunohistochemical markers.

AB - Introduction: Recent clinical trials revealed that accurate histologic typing of non-small cell lung cancer, especially squamous cell carcinoma (SCC), is essential. Patients and Methods: We analyzed 10 antibodies expression in 150 SCC cases (53 well-, 51 moderately, and 46 poorly differentiated cases) and 159 adenocarcinoma (AC) cases (49 well-, 52 moderately, and 58 poorly differentiated cases). Results: In all SCC and AC cases, p63 was the most sensitive marker for SCC (98.7%), followed by high-molecular-weight (HM) cytokeratin (CK) (97.3%), CK5/6 (93.3%), Sox2 (80%), thrombomodulin (79.3%), desmocollin-3 (72.7%), S100A7 (70.7%), S100A2 (63.3%), and glypican-3 (46.7%). Desmocollin-3 was the most specific marker for SCC (100%), followed by CK5/6 (98%), Sox2 (95.5%), glypican-3 (92.4%), S100A7 (86.8%), thrombomodulin (79.9%), S100A2 (64.6%), p63 (51.6%), and HMCK (33.3%). Thyroid transcription factor-1 (TTF-1) expression was observed in 87.4% of AC cases and 2.0% of SCC cases. When analyzing only poorly differentiated tumors, HMCK was the most sensitive marker for SCC (100%), followed by p63 (97.8%), CK5/6 (87.0%), Sox2 (71.7%), thrombomodulin (58.7%), desmocollin-3 (52.2%), S100A2 (50%), glypican-3 (45.7%), and S100A7 (45.7%). Desmocollin-3 was the most specific marker for poorly differentiated SCC (100%), followed by CK5/6 (98.3%), glypican-3 (94.8%), Sox2 (94.8%), S100A2 (81%), S100A7 (75.9%), thrombomodulin (72.4%), p63 (48.3%), and HMCK (36.8%). The 10-fold crossvalidated classification and regression tree analysis revealed that the combination of CK5/6 and TTF-1 was the best immunohistochemical marker panel for the differentiation between SCC and AC. Conclusion: CK5/6 is the best marker for differentiating SCC and AC, irrespective of the histological grade of the tumor. Thus, the combination of CK5/6 and TTF-1 is the most recommended combination of immunohistochemical markers.

KW - Cytokeratin 5/6

KW - Desmocollin-3

KW - Lung

KW - Sox2

KW - Squamous cell carcinoma

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