Utilizing mass spectrometry imaging to map the thyroid hormones triiodothyronine and thyroxine in Xenopus tropicalis tadpoles

Naoko Goto-Inoue, Tomohiko Sato, Mizuki Morisasa, Akihiko Kashiwagi, Keiko Kashiwagi, Yuki Sugiura, Eiji Sugiyama, Makoto Suematsu, Tsukasa Mori

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Thyroid hormones are not only responsible for thermogenesis and energy metabolism in animals, but also have an important role in cell differentiation and development. Amphibian metamorphosis provides an excellent model for studying the remodeling of the body. This metamorphic organ remodeling is induced by thyroid hormones, and a larval body is thus converted into an adult one. The matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry (MS) imaging technology is expected to be a suitable tool for investigating small bioreactive molecules. The present study describes the distribution of the thyroid hormones, i.e., triiodothyronine (T3) and thyroxine (T4) and their inactive form reverse T3 (rT3) in Xenopus tropicalis tadpoles using two different types of imaging techniques, MS/MS and Fourier transform (FT)-MS imaging. As a result of MS/MS imaging, we demonstrated that T3 was mainly distributed in the gills. T4 was faintly localized in the eyes, inner gills, and intestine during metamorphosis. The intensity of T3 in the gills and the intensity of T4 in the body fluids were increased during metamorphosis. Moreover, the localization of the inactive form rT3 was demonstrated to be separate from T3, namely in the intestine and muscles. In addition, FT-MS imaging could utilize simultaneous imaging including thyroid hormone. This is the first report to demonstrate the molecular distribution of thyroid hormones themselves and to discriminate T3, T4, and rT3 in animal tissues.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalAnalytical and Bioanalytical Chemistry
DOIs
Publication statusAccepted/In press - 2017 Dec 15

Fingerprint

Triiodothyronine
Xenopus
Thyroxine
Thyroid Hormones
Mass spectrometry
Larva
Mass Spectrometry
Imaging techniques
Fourier Analysis
Tandem Mass Spectrometry
Intestines
Fourier transforms
Animals
Thermogenesis
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Body Fluids
Amphibians
Energy Metabolism
Cell Differentiation
Body fluids

Keywords

  • Amphibian
  • Mass spectrometry imaging
  • Metamorphosis
  • Thyroid hormone

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

Utilizing mass spectrometry imaging to map the thyroid hormones triiodothyronine and thyroxine in Xenopus tropicalis tadpoles. / Goto-Inoue, Naoko; Sato, Tomohiko; Morisasa, Mizuki; Kashiwagi, Akihiko; Kashiwagi, Keiko; Sugiura, Yuki; Sugiyama, Eiji; Suematsu, Makoto; Mori, Tsukasa.

In: Analytical and Bioanalytical Chemistry, 15.12.2017, p. 1-8.

Research output: Contribution to journalArticle

Goto-Inoue, Naoko ; Sato, Tomohiko ; Morisasa, Mizuki ; Kashiwagi, Akihiko ; Kashiwagi, Keiko ; Sugiura, Yuki ; Sugiyama, Eiji ; Suematsu, Makoto ; Mori, Tsukasa. / Utilizing mass spectrometry imaging to map the thyroid hormones triiodothyronine and thyroxine in Xenopus tropicalis tadpoles. In: Analytical and Bioanalytical Chemistry. 2017 ; pp. 1-8.
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