UVB irradiation induces changes in cellular localization and phosphorylation of mouse HSP27

Junichi Nozaki, Makoto Takehana, Shizuko Kobayashi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We investigated the induction, cellular localization and phosphorylation of a low-molecular weight stress protein (heat shock protein 27, HSP27) by UVB (290-320 nm, max. 312 nm) irradiation stress using immunoblot and indirect immunofluorescence analysis in in vivo and in vitro experiments. The HSP27 was constitutively expressed and distributed in the cytoplasmic fraction of Pam 212 cells (mouse keratinocyte line) or dorsal skin. The increase in the cytoplasm HSP27 level induced by UVB irradiation was less than two-fold that in nonirradiated controls. On the other hand, the translocation of HSP27 from cytoplasm to the nucleus or perinuclear area was time- and dose-dependently induced by UVB irradiation. After UVB irradiation, three isoforms having different isoelectric points were detected in nucleic HSP27 by two-dimensional immunoblotting. The most basic isoform was the unphosphorylated type and the two acidic isoforms were phosphorylated, suggesting that HSP27 is phosphorylated in response to UVB irradiation and accumulates in or around the nucleus as a phosphorylated isoform. These results suggest that the translocation and phosphorylation of HSP27 are induced in response to UVB-irradiation stress.

Original languageEnglish
Pages (from-to)843-848
Number of pages6
JournalPhotochemistry and Photobiology
Volume65
Issue number5
Publication statusPublished - 1997 May

Fingerprint

HSP27 Heat-Shock Proteins
phosphorylation
Phosphorylation
mice
shock
Irradiation
proteins
heat
irradiation
Protein Isoforms
cytoplasm
Cytoplasm
Isoelectric Point
nuclei
Indirect Fluorescent Antibody Technique
Heat-Shock Proteins
Mouse Hspb2 protein
Keratinocytes
Immunoblotting
low molecular weights

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

UVB irradiation induces changes in cellular localization and phosphorylation of mouse HSP27. / Nozaki, Junichi; Takehana, Makoto; Kobayashi, Shizuko.

In: Photochemistry and Photobiology, Vol. 65, No. 5, 05.1997, p. 843-848.

Research output: Contribution to journalArticle

Nozaki, Junichi ; Takehana, Makoto ; Kobayashi, Shizuko. / UVB irradiation induces changes in cellular localization and phosphorylation of mouse HSP27. In: Photochemistry and Photobiology. 1997 ; Vol. 65, No. 5. pp. 843-848.
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