Uvb-irradiation induces phosphorylation of the 27-kda heat shock proto in in mouse kecatinocytes

S. Kobayashi, M. Sakamoto, J. Nozaki, M. Tanaka, M. Takehana

Research output: Contribution to journalArticle

Abstract

In the mouse ketatinocytes line Pam 212, the unphosphrylated 2 7-kDa heat shock protein (HSP27) isoform (pi 6.4) is constitutively expressed. Irradiat ion with ultraviolet B (UVB) osult s in the format ion of the phosphorylated HSP27 isoforms (pT 6.0) and the isoform level increases dose-dependently. The isoform can be Eliminated by the protein phosphatase 2A treamfiit . By prêtreatment with tumor necrosis factor alpha and okadaic acid, the level of the isoform increases 3 times that with irradiation only. These pretreated ketatinocytes are resistant aqa inst act in f ragp'entat ion by UVB-irradiation and shows an increase of translocation of HSP27 f rom the cytoplasm to the nucleus. These result suqqt'St that the phosphorylation of HSP27 may be related to the regulation of microfilament dynamies or apoptosis i'ol lowi ng irradiation stress.

Original languageEnglish
JournalFASEB Journal
Volume11
Issue number9
Publication statusPublished - 1997

Fingerprint

Phosphorylation
heat stress
Shock
phosphorylation
Protein Isoforms
Hot Temperature
irradiation
Irradiation
ions
ultraviolet radiation
mice
protein isoforms
phosphoprotein phosphatase
okadaic acid
Ions
alpha acids
microfilaments
heat shock proteins
tumor necrosis factor-alpha
HSP27 Heat-Shock Proteins

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Kobayashi, S., Sakamoto, M., Nozaki, J., Tanaka, M., & Takehana, M. (1997). Uvb-irradiation induces phosphorylation of the 27-kda heat shock proto in in mouse kecatinocytes. FASEB Journal, 11(9).

Uvb-irradiation induces phosphorylation of the 27-kda heat shock proto in in mouse kecatinocytes. / Kobayashi, S.; Sakamoto, M.; Nozaki, J.; Tanaka, M.; Takehana, M.

In: FASEB Journal, Vol. 11, No. 9, 1997.

Research output: Contribution to journalArticle

Kobayashi, S, Sakamoto, M, Nozaki, J, Tanaka, M & Takehana, M 1997, 'Uvb-irradiation induces phosphorylation of the 27-kda heat shock proto in in mouse kecatinocytes', FASEB Journal, vol. 11, no. 9.
Kobayashi, S. ; Sakamoto, M. ; Nozaki, J. ; Tanaka, M. ; Takehana, M. / Uvb-irradiation induces phosphorylation of the 27-kda heat shock proto in in mouse kecatinocytes. In: FASEB Journal. 1997 ; Vol. 11, No. 9.
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AU - Takehana, M.

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