Validity of plasma remnant lipoproteins as surrogate markers of antemortem level in cases of sudden coronary death

Sanae Takeichi, Yasuhiro Nakajima, Nobuhiro Yukawa, Masaki Q Fujita, Takeshi Saito, Fumiko Satoh, Yoshihisa Seto, Takahiko Kusakabe, Zheng Bin Jin, Iwao Hasegawa, Takamitsu Nakano, Abby Saniabadi, Masakazu Adachi, Naoki Ohara, Shinichi Usui, Mitsuyo Okazaki, Katsuyuki Nakajima

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Hitherto triglycerides (TG) and TG-rich lipoproteins were been of limited value as surrogates for antemortem levels. We measured TG levels in postmortem plasma from sudden coronary death cases (SCD, n=91) by using two TG assays, Dry Chem TG (free glycerol was added) and the Determiner L-TG (without added free glycerol) that measured net TG. TG levels were markedly higher by the Dry Chem TG (y) vs. Determiner L-TG (x), y=1.03x+229 mg/dl. HPLC showed large amounts of free glycerol in postmortem plasma and in TG-rich lipoprotein remnants (RLP). These results were verified in a rabbit model of SCD. Further, RLP from SCD were found to be biophysically similar to those from living patients with coronary artery disease (CAD). In conclusion, postmortem plasma sampled up to 12 h after death is appropriate for measuring lipid and lipoproteins, TG and RLP-TG as surrogates for antemortem levels when a TG assay without added free glycerol is used.

Original languageEnglish
Pages (from-to)93-103
Number of pages11
JournalClinica Chimica Acta
Volume343
Issue number1-2
DOIs
Publication statusPublished - 2004 May
Externally publishedYes

Fingerprint

Sudden Death
Lipoproteins
Triglycerides
Biomarkers
Plasmas
Glycerol
Assays
Coronary Artery Disease
High Pressure Liquid Chromatography
Rabbits

Keywords

  • Free glycerol
  • HPLC
  • Postmortem plasma
  • Remnant lipoproteins
  • Sudden coronary death
  • Triglycerides

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Validity of plasma remnant lipoproteins as surrogate markers of antemortem level in cases of sudden coronary death. / Takeichi, Sanae; Nakajima, Yasuhiro; Yukawa, Nobuhiro; Fujita, Masaki Q; Saito, Takeshi; Satoh, Fumiko; Seto, Yoshihisa; Kusakabe, Takahiko; Jin, Zheng Bin; Hasegawa, Iwao; Nakano, Takamitsu; Saniabadi, Abby; Adachi, Masakazu; Ohara, Naoki; Usui, Shinichi; Okazaki, Mitsuyo; Nakajima, Katsuyuki.

In: Clinica Chimica Acta, Vol. 343, No. 1-2, 05.2004, p. 93-103.

Research output: Contribution to journalArticle

Takeichi, S, Nakajima, Y, Yukawa, N, Fujita, MQ, Saito, T, Satoh, F, Seto, Y, Kusakabe, T, Jin, ZB, Hasegawa, I, Nakano, T, Saniabadi, A, Adachi, M, Ohara, N, Usui, S, Okazaki, M & Nakajima, K 2004, 'Validity of plasma remnant lipoproteins as surrogate markers of antemortem level in cases of sudden coronary death', Clinica Chimica Acta, vol. 343, no. 1-2, pp. 93-103. https://doi.org/10.1016/j.cccn.2003.12.031
Takeichi, Sanae ; Nakajima, Yasuhiro ; Yukawa, Nobuhiro ; Fujita, Masaki Q ; Saito, Takeshi ; Satoh, Fumiko ; Seto, Yoshihisa ; Kusakabe, Takahiko ; Jin, Zheng Bin ; Hasegawa, Iwao ; Nakano, Takamitsu ; Saniabadi, Abby ; Adachi, Masakazu ; Ohara, Naoki ; Usui, Shinichi ; Okazaki, Mitsuyo ; Nakajima, Katsuyuki. / Validity of plasma remnant lipoproteins as surrogate markers of antemortem level in cases of sudden coronary death. In: Clinica Chimica Acta. 2004 ; Vol. 343, No. 1-2. pp. 93-103.
@article{9da0fd13bfa645debf1336e07a0dfa25,
title = "Validity of plasma remnant lipoproteins as surrogate markers of antemortem level in cases of sudden coronary death",
abstract = "Hitherto triglycerides (TG) and TG-rich lipoproteins were been of limited value as surrogates for antemortem levels. We measured TG levels in postmortem plasma from sudden coronary death cases (SCD, n=91) by using two TG assays, Dry Chem TG (free glycerol was added) and the Determiner L-TG (without added free glycerol) that measured net TG. TG levels were markedly higher by the Dry Chem TG (y) vs. Determiner L-TG (x), y=1.03x+229 mg/dl. HPLC showed large amounts of free glycerol in postmortem plasma and in TG-rich lipoprotein remnants (RLP). These results were verified in a rabbit model of SCD. Further, RLP from SCD were found to be biophysically similar to those from living patients with coronary artery disease (CAD). In conclusion, postmortem plasma sampled up to 12 h after death is appropriate for measuring lipid and lipoproteins, TG and RLP-TG as surrogates for antemortem levels when a TG assay without added free glycerol is used.",
keywords = "Free glycerol, HPLC, Postmortem plasma, Remnant lipoproteins, Sudden coronary death, Triglycerides",
author = "Sanae Takeichi and Yasuhiro Nakajima and Nobuhiro Yukawa and Fujita, {Masaki Q} and Takeshi Saito and Fumiko Satoh and Yoshihisa Seto and Takahiko Kusakabe and Jin, {Zheng Bin} and Iwao Hasegawa and Takamitsu Nakano and Abby Saniabadi and Masakazu Adachi and Naoki Ohara and Shinichi Usui and Mitsuyo Okazaki and Katsuyuki Nakajima",
year = "2004",
month = "5",
doi = "10.1016/j.cccn.2003.12.031",
language = "English",
volume = "343",
pages = "93--103",
journal = "Clinica Chimica Acta",
issn = "0009-8981",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Validity of plasma remnant lipoproteins as surrogate markers of antemortem level in cases of sudden coronary death

AU - Takeichi, Sanae

AU - Nakajima, Yasuhiro

AU - Yukawa, Nobuhiro

AU - Fujita, Masaki Q

AU - Saito, Takeshi

AU - Satoh, Fumiko

AU - Seto, Yoshihisa

AU - Kusakabe, Takahiko

AU - Jin, Zheng Bin

AU - Hasegawa, Iwao

AU - Nakano, Takamitsu

AU - Saniabadi, Abby

AU - Adachi, Masakazu

AU - Ohara, Naoki

AU - Usui, Shinichi

AU - Okazaki, Mitsuyo

AU - Nakajima, Katsuyuki

PY - 2004/5

Y1 - 2004/5

N2 - Hitherto triglycerides (TG) and TG-rich lipoproteins were been of limited value as surrogates for antemortem levels. We measured TG levels in postmortem plasma from sudden coronary death cases (SCD, n=91) by using two TG assays, Dry Chem TG (free glycerol was added) and the Determiner L-TG (without added free glycerol) that measured net TG. TG levels were markedly higher by the Dry Chem TG (y) vs. Determiner L-TG (x), y=1.03x+229 mg/dl. HPLC showed large amounts of free glycerol in postmortem plasma and in TG-rich lipoprotein remnants (RLP). These results were verified in a rabbit model of SCD. Further, RLP from SCD were found to be biophysically similar to those from living patients with coronary artery disease (CAD). In conclusion, postmortem plasma sampled up to 12 h after death is appropriate for measuring lipid and lipoproteins, TG and RLP-TG as surrogates for antemortem levels when a TG assay without added free glycerol is used.

AB - Hitherto triglycerides (TG) and TG-rich lipoproteins were been of limited value as surrogates for antemortem levels. We measured TG levels in postmortem plasma from sudden coronary death cases (SCD, n=91) by using two TG assays, Dry Chem TG (free glycerol was added) and the Determiner L-TG (without added free glycerol) that measured net TG. TG levels were markedly higher by the Dry Chem TG (y) vs. Determiner L-TG (x), y=1.03x+229 mg/dl. HPLC showed large amounts of free glycerol in postmortem plasma and in TG-rich lipoprotein remnants (RLP). These results were verified in a rabbit model of SCD. Further, RLP from SCD were found to be biophysically similar to those from living patients with coronary artery disease (CAD). In conclusion, postmortem plasma sampled up to 12 h after death is appropriate for measuring lipid and lipoproteins, TG and RLP-TG as surrogates for antemortem levels when a TG assay without added free glycerol is used.

KW - Free glycerol

KW - HPLC

KW - Postmortem plasma

KW - Remnant lipoproteins

KW - Sudden coronary death

KW - Triglycerides

UR - http://www.scopus.com/inward/record.url?scp=11144357860&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=11144357860&partnerID=8YFLogxK

U2 - 10.1016/j.cccn.2003.12.031

DO - 10.1016/j.cccn.2003.12.031

M3 - Article

VL - 343

SP - 93

EP - 103

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

SN - 0009-8981

IS - 1-2

ER -