Variation in the safety of induced pluripotent stem cell lines

Kyoko Miura, Yohei Okada, Takashi Aoi, Aki Okada, Kazutoshi Takahashi, Keisuke Okita, Masato Nakagawa, Michiyo Koyanagi, Koji Tanabe, Mari Ohnuki, Daisuke Ogawa, Eiji Ikeda, Hideyuki Okano, Shinya Yamanaka

Research output: Contribution to journalArticle

626 Citations (Scopus)

Abstract

We evaluated the teratoma-forming propensity of secondary neurospheres (SNS) generated from 36 mouse induced pluripotent stem (iPS) cell lines derived in 11 different ways. Teratoma-formation of SNS from embryonic fibroblast-derived iPS cells was similar to that of SNS from embryonic stem (ES) cells. In contrast, SNS from iPS cells derived from different adult tissues varied substantially in their teratoma-forming propensity, which correlated with the persistence of undifferentiated cells.

Original languageEnglish
Pages (from-to)743-745
Number of pages3
JournalNature Biotechnology
Volume27
Issue number8
DOIs
Publication statusPublished - 2009 Aug

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Induced Pluripotent Stem Cells
Teratoma
Stem cells
Safety
Cell Line
Embryonic Stem Cells
Fibroblasts
Tissue

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biotechnology
  • Molecular Medicine
  • Bioengineering
  • Biomedical Engineering

Cite this

Miura, K., Okada, Y., Aoi, T., Okada, A., Takahashi, K., Okita, K., ... Yamanaka, S. (2009). Variation in the safety of induced pluripotent stem cell lines. Nature Biotechnology, 27(8), 743-745. https://doi.org/10.1038/nbt.1554

Variation in the safety of induced pluripotent stem cell lines. / Miura, Kyoko; Okada, Yohei; Aoi, Takashi; Okada, Aki; Takahashi, Kazutoshi; Okita, Keisuke; Nakagawa, Masato; Koyanagi, Michiyo; Tanabe, Koji; Ohnuki, Mari; Ogawa, Daisuke; Ikeda, Eiji; Okano, Hideyuki; Yamanaka, Shinya.

In: Nature Biotechnology, Vol. 27, No. 8, 08.2009, p. 743-745.

Research output: Contribution to journalArticle

Miura, K, Okada, Y, Aoi, T, Okada, A, Takahashi, K, Okita, K, Nakagawa, M, Koyanagi, M, Tanabe, K, Ohnuki, M, Ogawa, D, Ikeda, E, Okano, H & Yamanaka, S 2009, 'Variation in the safety of induced pluripotent stem cell lines', Nature Biotechnology, vol. 27, no. 8, pp. 743-745. https://doi.org/10.1038/nbt.1554
Miura K, Okada Y, Aoi T, Okada A, Takahashi K, Okita K et al. Variation in the safety of induced pluripotent stem cell lines. Nature Biotechnology. 2009 Aug;27(8):743-745. https://doi.org/10.1038/nbt.1554
Miura, Kyoko ; Okada, Yohei ; Aoi, Takashi ; Okada, Aki ; Takahashi, Kazutoshi ; Okita, Keisuke ; Nakagawa, Masato ; Koyanagi, Michiyo ; Tanabe, Koji ; Ohnuki, Mari ; Ogawa, Daisuke ; Ikeda, Eiji ; Okano, Hideyuki ; Yamanaka, Shinya. / Variation in the safety of induced pluripotent stem cell lines. In: Nature Biotechnology. 2009 ; Vol. 27, No. 8. pp. 743-745.
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