Vascular endothelial growth factor suppresses C-type natriuretic peptide secretion

Kentaro Doi, Hiroshi Itoh, Yasato Komatsu, Toshio Igaki, Tae Hwa Chun, Kazuhiko Takaya, Jun Yamashita, Mayumi Inoue, Takaaki Yoshimasa, Kazuwa Nakao

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Angiogenesis plays a pivotal role not only in wound healing and tumor progression but also in diabetic angiopathy, arteriosclerosis, and collateral formation of obstructive vascular diseases. Vascular endothelial growth factor (VEGF) is now thought to be an endothelium-specific and potent angiogenic factor. We previously demonstrated that C-type natriuretic peptide (CNP), originally isolated from porcine brain, is produced by endothelial cells and proposed that CNP can exert control over vascular tone and growth as a local vascular regulator. In the present study, we examined the effect of VEGF on CNP secretion from endothelial cells using the specific radioimmunoassay for CNP we developed. VEGF (1 to 100 ng/mL) dose-dependently suppressed CNP secretion from cultured bovine endothelial cells, and 100 ng/mL VEGF suppressed endothelial CNP secretion to 28% of control levels (31.7 ± 5.5 versus 8.9 ± 0.8 fmol/mL, vehicle versus VEGF). VEGF also suppressed CNP mRNA expression in endothelial cells 9 hours after administration. In contrast, basic fibroblast growth factor (20 ng/mL), an endothelium-nonspecific angiogenic factor, significantly stimulated CNP secretion by 290%. These results indicate that VEGF can regulate vascular tone and growth in the process of angiogenesis through suppression of endothelial secretion of CNP, which is an endothelium-derived vasorelaxing and growth-inhibitory peptide.

Original languageEnglish
Pages (from-to)811-815
Number of pages5
JournalHypertension
Volume27
Issue number3 II
Publication statusPublished - 1996 Mar
Externally publishedYes

Fingerprint

C-Type Natriuretic Peptide
Vascular Endothelial Growth Factor C
Vascular Endothelial Growth Factor A
Endothelial Cells
Endothelium
Blood Vessels
Angiogenesis Inducing Agents
Growth
Diabetic Angiopathies
Arteriosclerosis
Fibroblast Growth Factor 2
Vascular Diseases
Wound Healing
Radioimmunoassay
Swine

Keywords

  • cell division
  • endothelial cell
  • endothelial growth factors
  • natriuretic peptides

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Doi, K., Itoh, H., Komatsu, Y., Igaki, T., Chun, T. H., Takaya, K., ... Nakao, K. (1996). Vascular endothelial growth factor suppresses C-type natriuretic peptide secretion. Hypertension, 27(3 II), 811-815.

Vascular endothelial growth factor suppresses C-type natriuretic peptide secretion. / Doi, Kentaro; Itoh, Hiroshi; Komatsu, Yasato; Igaki, Toshio; Chun, Tae Hwa; Takaya, Kazuhiko; Yamashita, Jun; Inoue, Mayumi; Yoshimasa, Takaaki; Nakao, Kazuwa.

In: Hypertension, Vol. 27, No. 3 II, 03.1996, p. 811-815.

Research output: Contribution to journalArticle

Doi, K, Itoh, H, Komatsu, Y, Igaki, T, Chun, TH, Takaya, K, Yamashita, J, Inoue, M, Yoshimasa, T & Nakao, K 1996, 'Vascular endothelial growth factor suppresses C-type natriuretic peptide secretion', Hypertension, vol. 27, no. 3 II, pp. 811-815.
Doi K, Itoh H, Komatsu Y, Igaki T, Chun TH, Takaya K et al. Vascular endothelial growth factor suppresses C-type natriuretic peptide secretion. Hypertension. 1996 Mar;27(3 II):811-815.
Doi, Kentaro ; Itoh, Hiroshi ; Komatsu, Yasato ; Igaki, Toshio ; Chun, Tae Hwa ; Takaya, Kazuhiko ; Yamashita, Jun ; Inoue, Mayumi ; Yoshimasa, Takaaki ; Nakao, Kazuwa. / Vascular endothelial growth factor suppresses C-type natriuretic peptide secretion. In: Hypertension. 1996 ; Vol. 27, No. 3 II. pp. 811-815.
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